##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727429.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2699611
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.663167397080542	31.0	30.0	31.0	27.0	34.0
2	30.970086060547242	31.0	31.0	34.0	27.0	34.0
3	31.04486424155184	31.0	31.0	34.0	27.0	34.0
4	34.89004971456999	35.0	35.0	37.0	32.0	37.0
5	34.64780592463136	35.0	35.0	37.0	32.0	37.0
6	34.74965726543565	35.0	35.0	37.0	32.0	37.0
7	34.688896289132025	35.0	35.0	37.0	31.0	37.0
8	34.68076919230215	35.0	35.0	37.0	30.0	37.0
9	36.232186785429455	38.0	35.0	39.0	32.0	39.0
10	35.91817080312682	37.0	35.0	39.0	30.0	39.0
11	36.095963085051885	38.0	35.0	39.0	31.0	39.0
12	35.88247936461957	37.0	35.0	39.0	30.0	39.0
13	36.02907159587066	37.0	35.0	39.0	30.0	39.0
14	36.97733932777722	39.0	36.0	40.0	31.0	41.0
15	36.92701763328124	38.0	36.0	40.0	31.0	41.0
16	36.88377658855294	38.0	36.0	40.0	31.0	41.0
17	36.81673359606254	38.0	36.0	40.0	31.0	41.0
18	36.7952705037874	38.0	36.0	40.0	31.0	41.0
19	36.78847359860365	39.0	36.0	40.0	30.0	41.0
20	36.749800989846314	39.0	36.0	40.0	30.0	41.0
21	36.65552370322983	38.0	35.0	40.0	30.0	41.0
22	36.57616782566081	38.0	35.0	40.0	30.0	41.0
23	36.480567385449234	38.0	35.0	40.0	30.0	41.0
24	36.37728287519943	38.0	35.0	40.0	30.0	41.0
25	36.26866796734789	38.0	35.0	40.0	30.0	41.0
26	36.02725874209284	38.0	34.0	40.0	29.0	41.0
27	35.91924836578307	38.0	34.0	40.0	28.0	41.0
28	35.74581337829784	38.0	34.0	40.0	27.0	41.0
29	35.577410226880836	38.0	34.0	40.0	27.0	41.0
30	35.728863528856564	38.0	34.0	40.0	27.0	41.0
31	35.7257508581792	38.0	34.0	40.0	27.0	41.0
32	35.67987980490523	38.0	34.0	40.0	27.0	41.0
33	35.5664949505688	38.0	34.0	40.0	27.0	41.0
34	35.52421700756146	38.0	34.0	40.0	27.0	41.0
35	35.41429228136943	38.0	34.0	40.0	27.0	41.0
36	35.31237648683459	38.0	34.0	40.0	26.0	41.0
37	35.21976314365292	38.0	33.0	40.0	26.0	41.0
38	35.09368090439697	38.0	33.0	40.0	26.0	41.0
39	34.997331096961744	38.0	33.0	40.0	25.0	41.0
40	34.82716102431054	38.0	33.0	40.0	25.0	41.0
41	34.66752061685924	38.0	33.0	40.0	25.0	41.0
42	34.54023820468949	37.0	33.0	40.0	24.0	41.0
43	34.36907502599448	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	4.0
13	10.0
14	27.0
15	60.0
16	155.0
17	267.0
18	440.0
19	881.0
20	1733.0
21	3142.0
22	5492.0
23	9314.0
24	14667.0
25	21634.0
26	31000.0
27	42588.0
28	57090.0
29	74271.0
30	92933.0
31	113935.0
32	137232.0
33	164526.0
34	198055.0
35	238610.0
36	296110.0
37	381160.0
38	521052.0
39	293216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.44116393065519	22.79561759083068	13.54547006957669	24.217748408937435
2	21.788731783949615	23.842101695392408	30.72350053396582	23.64566598669216
3	23.257647120270292	24.796313246612197	28.89801530664974	23.048024326467775
4	17.92017442513014	18.2551486121519	31.983385754466102	31.841291208251853
5	14.944856870119436	37.6719831116409	33.20533958411045	14.177820434129213
6	34.662216148919235	35.650210345120094	14.959155226438178	14.728418279522495
7	28.0882690135727	31.43645510408722	21.055589120062113	19.419686762277973
8	27.89275936421951	33.402553182662245	19.763921542770422	18.940765910347825
9	26.75874413017283	13.549841069694857	19.107160253829164	40.58425454630315
10	17.335571680512487	27.124018979030684	32.27828009294672	23.262129247510106
11	36.92798703220575	21.37867270506751	20.484617969033316	21.208722293693423
12	21.16038199577643	26.494854258632078	28.67827994477723	23.666483800814262
13	31.089738484544625	19.209471290493337	24.66681310751808	25.033977117443957
14	22.902447797108547	21.000358940602922	24.359805912777805	31.737387349510726
15	26.026897949371225	27.72666135972924	21.31577475421459	24.93066593668495
16	25.986299507595724	25.56109009779557	23.958599961253675	24.49401043335503
17	24.049724201005258	27.28070822055474	24.472192475138087	24.197375103301923
18	25.132732086215388	23.879032942153515	26.0518645093682	24.936370462262897
19	24.83065152720151	25.859799800786114	25.421588517753115	23.88796015425926
20	24.83291111200836	24.402960278351216	25.531382113941604	25.232746495698827
21	25.811829926607942	25.159439637784853	25.04427489738336	23.98445553822384
22	25.1372142134552	25.925475929680243	25.213928969766386	23.723380887098177
23	24.47956390754075	24.362806345062307	25.79149366334631	25.366136084050627
24	24.655663352979374	26.15736119018629	25.463557527362273	23.72341792947206
25	25.11961908586089	25.3079795570547	24.87417631651375	24.69822504057066
26	25.222856181872128	25.713741720566407	25.35435660915591	23.709045488405554
27	25.431441789205927	25.44618465401126	24.996823616439553	24.12554994034326
28	25.043348838036295	24.804573695988054	26.179660699263707	23.972416766711945
29	24.292166538067892	25.253193886082105	25.86254093645344	24.592098639396564
30	24.31124336061751	25.9098810902756	26.100056637789663	23.678818911317222
31	25.643731633928002	25.373692728322712	24.777162339314813	24.20541329843448
32	24.457116228967806	25.627840455532297	25.186962121579736	24.72808119392016
33	24.601618529484433	24.94288992006626	25.93369933668221	24.521792213767093
34	25.05486901631383	25.553755707766783	25.494043401067785	23.8973318748516
35	25.216929402050887	25.08413249168121	26.537712285214425	23.161225821053478
36	24.74230546549114	25.659659854697587	25.291273446433575	24.306761233377696
37	24.836059713788394	25.595613590254302	25.64347233731082	23.924854358646485
38	24.22934267196274	24.7597894659638	26.4634423255795	24.54742553649396
39	25.25367543694258	24.54809229922385	25.186776909710325	25.011455354123243
40	24.61850985197497	25.031680490263224	26.606796312505764	23.74301334525604
41	24.105991566933163	25.23733975006029	26.057865373937204	24.598803309069346
42	25.401289296865365	25.312832108033344	26.16795530911676	23.11792328598454
43	24.807981594385264	24.177149967161935	26.392691391463437	24.622177046989364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	221.0
1	249.0
2	277.0
3	491.5
4	706.0
5	706.0
6	1381.5
7	2057.0
8	1955.5
9	1854.0
10	2496.0
11	3138.0
12	3138.0
13	6066.0
14	8994.0
15	13671.5
16	18349.0
17	16010.0
18	13671.0
19	13671.0
20	17405.5
21	21140.0
22	19318.5
23	17497.0
24	21339.5
25	25182.0
26	25182.0
27	30708.0
28	36234.0
29	40627.0
30	45020.0
31	51554.0
32	58088.0
33	58088.0
34	68293.5
35	78499.0
36	92299.0
37	106099.0
38	117285.5
39	128472.0
40	128472.0
41	139129.5
42	149787.0
43	158047.5
44	166308.0
45	181057.5
46	195807.0
47	195807.0
48	219240.5
49	242674.0
50	241484.5
51	240295.0
52	232780.0
53	225265.0
54	225265.0
55	211043.5
56	196822.0
57	194625.5
58	192429.0
59	177164.0
60	161899.0
61	161899.0
62	136444.5
63	110990.0
64	98870.5
65	86751.0
66	74187.5
67	61624.0
68	61624.0
69	50051.5
70	38479.0
71	33724.5
72	28970.0
73	21432.0
74	13894.0
75	13894.0
76	10802.0
77	7710.0
78	6592.5
79	5475.0
80	4461.5
81	3448.0
82	3448.0
83	2860.0
84	2272.0
85	2066.0
86	1860.0
87	1286.0
88	712.0
89	712.0
90	531.5
91	351.0
92	250.0
93	149.0
94	134.5
95	120.0
96	120.0
97	63.5
98	7.0
99	11.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2699611.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.36543437768825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.00190657032351	48.475348211456456
2	8.094107324116017	9.124557504468543
3	2.3488972400439843	3.9718983973089665
4	1.074238323479122	2.421996389122412
5	0.5860235581334017	1.6515736204873814
6	0.3753094928774865	1.269268955526565
7	0.26052745638326047	1.0279320272449692
8	0.1807333624630377	0.8149691585415435
9	0.12873130371916464	0.6530396266923146
>10	0.7969979537913982	8.324270648932899
>50	0.06878367787328163	2.720732448069207
>100	0.06565888170484784	7.944457433041261
>500	0.011859995549153101	4.680860073565674
>1k	0.006028285030504934	5.98061884921753
>5k	1.9657451186429133E-4	0.9384766563244452
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT	8681	0.32156484767620225	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8424	0.312044957588334	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8305	0.3076369150962861	No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATG	4703	0.17421028437060007	No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT	4296	0.1591340382003185	RNA PCR Primer, Index 23 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4275	0.15835614834878062	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	4127	0.1528738770141328	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	4081	0.15116992781552602	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3859	0.14294652081355425	No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC	3666	0.1357973426541824	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3435	0.12724055428726583	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	3320	0.12298068129074893	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	3267	0.12101743547496288	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	2809	0.10405202823666077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.408474776551141E-5
2	0.0	7.408474776551141E-5	0.0	0.0	7.408474776551141E-5
3	0.0	7.408474776551141E-5	0.0	0.0	7.408474776551141E-5
4	0.0	1.111271216482671E-4	0.0	3.7042373882755704E-5	7.408474776551141E-5
5	0.0	1.111271216482671E-4	0.0	3.7042373882755704E-5	7.408474776551141E-5
6	0.0	1.111271216482671E-4	0.0	3.7042373882755704E-5	1.111271216482671E-4
7	0.0	1.111271216482671E-4	0.0	7.408474776551141E-5	1.111271216482671E-4
8	0.0	1.111271216482671E-4	0.0	7.408474776551141E-5	1.111271216482671E-4
9	0.0	1.111271216482671E-4	0.0	1.111271216482671E-4	1.111271216482671E-4
10	0.0	1.111271216482671E-4	0.0	1.852118694137785E-4	1.111271216482671E-4
11	0.0	1.111271216482671E-4	0.0	2.222542432965342E-4	1.111271216482671E-4
12	0.0	1.111271216482671E-4	0.0	2.592966171792899E-4	1.111271216482671E-4
13	0.0	1.4816949553102281E-4	0.0	2.592966171792899E-4	1.111271216482671E-4
14	0.0	1.852118694137785E-4	0.0	2.9633899106204563E-4	1.4816949553102281E-4
15	0.0	2.222542432965342E-4	0.0	3.333813649448013E-4	2.222542432965342E-4
16	0.0	2.222542432965342E-4	0.0	3.70423738827557E-4	2.222542432965342E-4
17	0.0	2.222542432965342E-4	0.0	4.445084865930684E-4	2.222542432965342E-4
18	0.0	2.222542432965342E-4	0.0	5.185932343585798E-4	2.222542432965342E-4
19	0.0	2.222542432965342E-4	0.0	6.297203560068469E-4	2.222542432965342E-4
20	0.0	2.222542432965342E-4	0.0	6.667627298896026E-4	2.222542432965342E-4
21	0.0	2.222542432965342E-4	0.0	7.40847477655114E-4	2.222542432965342E-4
22	0.0	2.222542432965342E-4	0.0	0.001000144094834404	2.222542432965342E-4
23	0.0	2.222542432965342E-4	0.0	0.0012964830858964494	2.222542432965342E-4
24	0.0	2.222542432965342E-4	0.0	0.001963245815786052	2.222542432965342E-4
25	0.0	2.592966171792899E-4	0.0	0.0022966271807308533	2.222542432965342E-4
26	0.0	2.592966171792899E-4	0.0	0.003222686527799746	2.592966171792899E-4
27	0.0	2.592966171792899E-4	0.0	0.00577861032570989	2.592966171792899E-4
28	0.0	2.592966171792899E-4	0.0	0.012001729138012847	2.592966171792899E-4
29	0.0	2.592966171792899E-4	0.0	0.020558517504929413	2.592966171792899E-4
30	0.0	2.592966171792899E-4	0.0	0.03481983144979036	2.592966171792899E-4
31	0.0	2.592966171792899E-4	0.0	0.07512193423422855	2.592966171792899E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCGA	25	0.0054976405	29.6	6
CTTATAC	1440	0.0	25.565971	1
TATACAC	1505	0.0	25.44518	3
TTATACA	1570	0.0	23.802547	2
GCCGTCT	780	0.0	21.583334	36
CAAGACG	970	0.0	18.690722	4
AAGACGG	1005	0.0	17.67164	5
TACACAT	2225	0.0	17.294382	5
CCGTCTT	1175	0.0	16.217022	37
ATACACA	2385	0.0	16.056604	4
TGCCGTC	1035	0.0	15.908212	35
CGCAAGA	1095	0.0	15.712328	2
ATGCCGT	1055	0.0	15.606634	34
TATGCCG	1080	0.0	15.587963	33
ATACCGT	755	0.0	15.437087	6
TACACCG	120	1.9377567E-6	15.416666	5
TACCGTC	720	0.0	15.159722	7
GCGCAAG	1175	0.0	15.114894	1
ACACATC	2635	0.0	15.094876	6
ACGGACC	1135	0.0	14.832599	8
>>END_MODULE