FastQCFastQC Report
Thu 9 Feb 2017
SRR2727428.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727428.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4788153
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG171050.3572358694469454No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC162920.340256462147304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT150520.31435921116138105No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT146590.3061514533892296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT144950.30272633309754304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105590.220523446097065No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA101540.21206506976698533No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG88620.18508180503003976No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC64920.13558463983920313No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT61650.12875528413565732No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA60530.12641617759499332No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT57950.12102787859953515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57500.12008805900730407No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG56590.11818753494301457No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC55260.11540984592597606No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG54900.11465799025219119No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG54840.11453268097322705No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT51120.10676350567745015RNA PCR Primer, Index 39 (95% over 23bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG50850.10619961392211151No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC49660.103714313222656No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG48760.10183467403819385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC24350.024.8439431
TATACAC26050.024.0748583
TTATACA25950.023.6685922
TACACAT30750.020.154475
ATACACA36000.018.0888884
ACACATC36000.017.2666666
ATCTCCG36550.016.90560710
GCCGTCT10050.016.75124536
CACATCT39450.015.8504437
TCTAGCG11950.014.86192528
CGCAATA11600.014.83189736
ATCTCGT41850.014.76463737
TAGTACT3800.014.6052644
CACGAGA41850.014.54360921
CGAGACC41900.014.43794723
CTAGCGG12350.014.38056829
TCTCCGA43700.014.30892611
AAGACGG15950.014.150475
CCCACGA43350.014.12572119
CATCTCG43600.014.04472436
Produced by FastQC (version 0.11.5)