FastQCFastQC Report
Thu 9 Feb 2017
SRR2727427.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727427.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3900811
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82090.21044341804819564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74200.19021685490530046No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54750.1403554286531698No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC53720.13771495209585904No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG49200.12612761807736905No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT48910.125384182930165No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT48450.1242049409725311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC11700.019.1324793
CAAGACG13950.018.4336914
AAGACGG13500.018.3629655
ACGGACC13200.017.9393948
GACGGAC13850.017.4981967
TTATACA12950.016.571432
GCGCAAG15950.015.8902831
CGGACCA15150.015.7524769
CTTATAC12800.015.4648441
AGACGGA15650.015.3674136
TAGACGG855.368241E-415.2352945
CGCAAGA16350.014.7094792
ATCTCCG13850.014.292418510
GAGCGAA16650.014.22222216
AGCGAAA16800.014.09523817
CGAACGA6000.013.87516
AGAGCGA17100.013.84795315
GCAAGAC19950.013.8170423
TCTAGCG7800.013.75641128
CAGAGCG17150.013.69970814
Produced by FastQC (version 0.11.5)