FastQCFastQC Report
Thu 9 Feb 2017
SRR2727424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4583175
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT121460.26501279135097394No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG112820.24616123102434448No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC110320.2407064971335373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79250.17291506433858625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72150.15742362008869398No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA66290.14463772384864204No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG56530.12334244273893098No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT54280.11843318223720455No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53500.11673130526327273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC21350.025.9086653
CTTATAC20050.025.6508711
TTATACA22350.023.673382
TACACAT28050.019.5882345
ATACACA31200.018.6778854
ATCTCCG30700.017.17426710
AAGACGG14000.016.655
GCCGTCT7450.016.63758536
CAAGACG14550.016.4020614
ACACATC33850.016.3958646
ACATCTC35200.015.7670458
CACGAGA34750.015.225921
CGAGACA34800.015.15086123
CGCAATA9600.015.03125136
CCCACGA35500.014.95633819
TATGCCG7800.014.94230833
TCTCCGA35900.014.89275811
ATCTCGT34100.014.86510337
TCCGAGC37050.014.53036413
GCCCACG36950.014.51962118
Produced by FastQC (version 0.11.5)