##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727424.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4583175
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.239957671265007	31.0	31.0	34.0	30.0	34.0
2	31.53651911611492	31.0	31.0	34.0	30.0	34.0
3	31.65790440033383	31.0	31.0	34.0	30.0	34.0
4	35.442461175931534	37.0	35.0	37.0	33.0	37.0
5	35.22758568023259	37.0	35.0	37.0	32.0	37.0
6	35.216025789981835	37.0	35.0	37.0	32.0	37.0
7	35.14226535098485	37.0	35.0	37.0	32.0	37.0
8	35.1267328871361	37.0	35.0	37.0	32.0	37.0
9	36.75769264756419	39.0	37.0	39.0	32.0	39.0
10	36.527361490669676	39.0	35.0	39.0	32.0	39.0
11	36.638378634898295	39.0	35.0	39.0	32.0	39.0
12	36.47110551091765	38.0	35.0	39.0	32.0	39.0
13	36.586417494422534	39.0	35.0	39.0	32.0	39.0
14	37.69072684329095	39.0	37.0	41.0	32.0	41.0
15	37.642599507983	39.0	36.0	41.0	32.0	41.0
16	37.610024709944526	39.0	36.0	41.0	32.0	41.0
17	37.49375160232808	39.0	36.0	41.0	32.0	41.0
18	37.49792971029908	39.0	36.0	41.0	32.0	41.0
19	37.4992377554861	39.0	36.0	41.0	32.0	41.0
20	37.45941579800029	39.0	36.0	41.0	32.0	41.0
21	37.39904389424362	39.0	36.0	41.0	31.0	41.0
22	37.32468627098027	39.0	36.0	41.0	31.0	41.0
23	37.20962760531727	39.0	36.0	41.0	31.0	41.0
24	37.16443491684258	39.0	36.0	41.0	31.0	41.0
25	37.06955942114364	39.0	36.0	41.0	31.0	41.0
26	36.87560217534788	39.0	36.0	40.0	30.0	41.0
27	36.81305405095812	39.0	36.0	40.0	30.0	41.0
28	36.68555182815406	39.0	35.0	40.0	30.0	41.0
29	36.558466128829906	39.0	35.0	40.0	30.0	41.0
30	36.68442902572998	39.0	35.0	40.0	30.0	41.0
31	36.6741719441217	39.0	35.0	40.0	30.0	41.0
32	36.654958407654085	39.0	35.0	40.0	30.0	41.0
33	36.55296688431055	39.0	35.0	40.0	30.0	41.0
34	36.530688223775	39.0	35.0	40.0	30.0	41.0
35	36.446504879259464	39.0	35.0	40.0	30.0	41.0
36	36.37572359772429	38.0	35.0	40.0	30.0	41.0
37	36.302638672972336	38.0	35.0	40.0	30.0	41.0
38	36.194288239048255	38.0	35.0	40.0	29.0	41.0
39	36.09024682670856	38.0	35.0	40.0	29.0	41.0
40	35.97302634090996	38.0	35.0	40.0	28.0	41.0
41	35.8434089904924	38.0	34.0	40.0	28.0	41.0
42	35.73789938197865	38.0	34.0	40.0	27.0	41.0
43	35.580667768522915	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	6.0
14	9.0
15	36.0
16	105.0
17	231.0
18	340.0
19	715.0
20	1421.0
21	2733.0
22	5091.0
23	8791.0
24	14542.0
25	22718.0
26	34586.0
27	49966.0
28	69879.0
29	95123.0
30	124127.0
31	156655.0
32	193602.0
33	236473.0
34	291202.0
35	356341.0
36	444090.0
37	571167.0
38	839274.0
39	1063950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.04216509297594	19.32614399406525	11.926819290120932	26.704871622837878
2	18.73378171245916	21.693236675448787	33.192252095981495	26.380729516110556
3	20.39291539162262	23.6592318643735	29.450653749856816	26.49719899414707
4	14.977390128022606	16.234662651982525	33.70890267118319	35.07904454881169
5	14.93903243930245	36.25840165387532	33.80985888603424	14.992707020787991
6	35.172691420249066	34.63369825503062	15.49855722288588	14.695053101834427
7	28.849803902316623	30.46859436962368	21.241693803967774	19.439907924091923
8	27.934477736503627	33.011460395904585	19.931292171911394	19.122769695680397
9	26.853262203603396	13.793036486714996	18.890703497029897	40.46299781265171
10	17.181779006911146	26.369012747713107	32.56727050570838	23.881937739667368
11	37.002078253612396	21.083288331778736	20.678481620274155	21.236151794334713
12	21.793712873717457	25.46778161427395	28.87208103552668	23.866424476481914
13	30.78813268094716	18.918129899032877	24.459310412541523	25.83442700747844
14	22.94350532109291	20.41434158634571	24.124106105483644	32.51804698707774
15	26.074740763771842	26.98371761933594	21.293186491896993	25.648355124995227
16	26.00127640773045	25.21383102325353	23.92692838479875	24.857964184217273
17	24.744374805675104	26.25060138441146	24.46005225635067	24.544971553562757
18	25.641591254970624	23.85431496724432	25.656428131153618	24.847665646631427
19	25.255505190179296	25.44148979692026	24.971051727241488	24.331953285658958
20	25.13953754766074	24.147496004407426	25.126991659711884	25.585974788219957
21	25.757908000458198	24.872822879335832	24.816617301324957	24.552651818881017
22	25.318758284377097	24.884692380282228	24.715093794149254	25.08145554119142
23	24.277165938459692	24.185613684836387	25.322685692778478	26.214534683925443
24	24.964615141250334	25.658239102805368	24.835730688878343	24.54141506706595
25	25.29979762937265	24.831017798796687	24.3080179133461	25.561166658484563
26	25.456653957136698	25.251381411357848	24.852094890550763	24.439869740954688
27	25.886792452830186	24.69224936861455	24.300926759288046	25.120031419267214
28	24.80908976855564	24.721661293753787	25.556628319887416	24.91262061780316
29	24.764644596813344	24.918599006147485	25.30121586018426	25.015540536854907
30	24.58376562099418	25.45002100072548	25.646936894183618	24.319276484096726
31	25.90282937046916	24.76124084286548	24.172631418176262	25.163298368489095
32	24.348797503913772	24.97545915222526	24.70352975830074	25.972213585560226
33	24.39697371363738	24.90147114173035	25.295586574808947	25.405968569823322
34	25.46998532676583	24.7378509439417	25.048814413588833	24.743349315703632
35	25.282124291566436	25.12310788918163	25.762140873957463	23.832626945294475
36	24.841359974253656	25.43247857653264	24.78683445428115	24.939326994932554
37	25.12000960033165	25.09912887899764	25.191553890043473	24.589307630627243
38	24.663011994959827	24.57423074615305	25.808658844578268	24.95409841430886
39	25.364578049059876	24.112716621119638	24.828181337173465	25.694523992647017
40	25.176302454084777	24.80239135533773	25.876624828857725	24.14468136171977
41	24.395664577503588	24.82706857145974	25.537754940625224	25.23951191041145
42	25.850114822148402	24.980390231662547	25.537994948916413	23.631499997272634
43	25.077833597887928	23.841005416550754	25.681214441953447	25.39994654360787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	177.0
1	228.5
2	280.0
3	732.5
4	1185.0
5	1185.0
6	1758.5
7	2332.0
8	2094.5
9	1857.0
10	2543.5
11	3230.0
12	3230.0
13	5720.0
14	8210.0
15	12272.0
16	16334.0
17	15763.5
18	15193.0
19	15193.0
20	18917.0
21	22641.0
22	22251.5
23	21862.0
24	27804.0
25	33746.0
26	33746.0
27	41976.0
28	50206.0
29	57791.0
30	65376.0
31	76013.5
32	86651.0
33	86651.0
34	103775.5
35	120900.0
36	144567.0
37	168234.0
38	192880.0
39	217526.0
40	217526.0
41	234471.5
42	251417.0
43	266185.0
44	280953.0
45	308093.5
46	335234.0
47	335234.0
48	385928.0
49	436622.0
50	446161.5
51	455701.0
52	411743.0
53	367785.0
54	367785.0
55	362107.5
56	356430.0
57	343041.0
58	329652.0
59	306357.0
60	283062.0
61	283062.0
62	241249.0
63	199436.0
64	175659.0
65	151882.0
66	129763.0
67	107644.0
68	107644.0
69	88902.0
70	70160.0
71	60981.5
72	51803.0
73	39021.5
74	26240.0
75	26240.0
76	20909.0
77	15578.0
78	13276.5
79	10975.0
80	8970.0
81	6965.0
82	6965.0
83	5830.0
84	4695.0
85	3948.5
86	3202.0
87	2089.0
88	976.0
89	976.0
90	688.0
91	400.0
92	298.5
93	197.0
94	204.0
95	211.0
96	211.0
97	109.0
98	7.0
99	7.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4583175.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.13121328209828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.04950239615442	42.7734010910396
2	10.098673631545488	10.529122179047997
3	3.182959451240138	4.977946140626104
4	1.4537547180279506	3.031439890614869
5	0.7921544653035649	2.064798669155333
6	0.4969515864796814	1.5544013487389625
7	0.35205527050185487	1.2847147875533298
8	0.24878707867928315	1.03756578083679
9	0.1841350284184543	0.8639264195268901
>10	0.993057417622272	9.300991570925365
>50	0.06973966059502665	2.5314468691098746
>100	0.05829302329205127	6.352440524420048
>500	0.010992116791286805	4.140191599978808
>1k	0.008567999282492307	7.974475080307883
>5k	2.5077071070708844E-4	0.8323131494373159
>10k+	1.2538535535354422E-4	0.7508248986808876
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	12146	0.26501279135097394	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	11282	0.24616123102434448	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	11032	0.2407064971335373	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7925	0.17291506433858625	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7215	0.15742362008869398	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	6629	0.14463772384864204	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	5653	0.12334244273893098	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5428	0.11843318223720455	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5350	0.11673130526327273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.181893556322855E-5
2	0.0	2.181893556322855E-5	0.0	0.0	2.181893556322855E-5
3	0.0	2.181893556322855E-5	0.0	0.0	2.181893556322855E-5
4	0.0	4.36378711264571E-5	0.0	6.545680668968565E-5	2.181893556322855E-5
5	0.0	4.36378711264571E-5	0.0	1.0909467781614274E-4	2.181893556322855E-5
6	2.181893556322855E-5	4.36378711264571E-5	0.0	1.0909467781614274E-4	4.36378711264571E-5
7	2.181893556322855E-5	6.545680668968565E-5	0.0	1.0909467781614274E-4	4.36378711264571E-5
8	2.181893556322855E-5	6.545680668968565E-5	0.0	1.0909467781614274E-4	4.36378711264571E-5
9	2.181893556322855E-5	1.309136133793713E-4	0.0	1.309136133793713E-4	4.36378711264571E-5
10	2.181893556322855E-5	1.309136133793713E-4	0.0	1.9637042006905692E-4	6.545680668968565E-5
11	2.181893556322855E-5	1.309136133793713E-4	0.0	1.9637042006905692E-4	6.545680668968565E-5
12	2.181893556322855E-5	1.5273254894259983E-4	0.0	1.9637042006905692E-4	8.72757422529142E-5
13	2.181893556322855E-5	1.5273254894259983E-4	0.0	1.9637042006905692E-4	8.72757422529142E-5
14	2.181893556322855E-5	1.5273254894259983E-4	0.0	2.4000829119551401E-4	1.0909467781614274E-4
15	2.181893556322855E-5	1.5273254894259983E-4	0.0	2.4000829119551401E-4	1.0909467781614274E-4
16	4.36378711264571E-5	1.5273254894259983E-4	0.0	3.0546509788519966E-4	1.0909467781614274E-4
17	4.36378711264571E-5	1.5273254894259983E-4	0.0	3.491029690116568E-4	1.0909467781614274E-4
18	4.36378711264571E-5	1.5273254894259983E-4	0.0	3.9274084013811385E-4	1.0909467781614274E-4
19	4.36378711264571E-5	1.5273254894259983E-4	0.0	4.8001658239102803E-4	1.0909467781614274E-4
20	4.36378711264571E-5	1.5273254894259983E-4	0.0	5.454733890807137E-4	1.5273254894259983E-4
21	4.36378711264571E-5	1.5273254894259983E-4	0.0	6.109301957703993E-4	1.745514845058284E-4
22	4.36378711264571E-5	1.5273254894259983E-4	0.0	8.509384869659133E-4	1.745514845058284E-4
23	4.36378711264571E-5	1.745514845058284E-4	0.0	0.001112765713724656	1.745514845058284E-4
24	4.36378711264571E-5	1.745514845058284E-4	0.0	0.0015491444249892269	1.9637042006905692E-4
25	4.36378711264571E-5	1.745514845058284E-4	0.0	0.001876428458437655	1.9637042006905692E-4
26	4.36378711264571E-5	1.745514845058284E-4	0.0	0.0023782639763919116	1.9637042006905692E-4
27	4.36378711264571E-5	1.745514845058284E-4	0.0	0.003861951594691453	1.9637042006905692E-4
28	4.36378711264571E-5	1.745514845058284E-4	0.0	0.007920273609451963	1.9637042006905692E-4
29	4.36378711264571E-5	1.745514845058284E-4	0.0	0.013462283242512014	1.9637042006905692E-4
30	4.36378711264571E-5	1.9637042006905692E-4	0.0	0.023368079988217775	1.9637042006905692E-4
31	4.36378711264571E-5	1.9637042006905692E-4	0.0	0.05240908322287497	2.1818935563228548E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	2135	0.0	25.908665	3
CTTATAC	2005	0.0	25.650871	1
TTATACA	2235	0.0	23.67338	2
TACACAT	2805	0.0	19.588234	5
ATACACA	3120	0.0	18.677885	4
ATCTCCG	3070	0.0	17.174267	10
AAGACGG	1400	0.0	16.65	5
GCCGTCT	745	0.0	16.637585	36
CAAGACG	1455	0.0	16.402061	4
ACACATC	3385	0.0	16.395864	6
ACATCTC	3520	0.0	15.767045	8
CACGAGA	3475	0.0	15.2259	21
CGAGACA	3480	0.0	15.150861	23
CGCAATA	960	0.0	15.031251	36
CCCACGA	3550	0.0	14.956338	19
TATGCCG	780	0.0	14.942308	33
TCTCCGA	3590	0.0	14.892758	11
ATCTCGT	3410	0.0	14.865103	37
TCCGAGC	3705	0.0	14.530364	13
GCCCACG	3695	0.0	14.519621	18
>>END_MODULE