FastQCFastQC Report
Thu 9 Feb 2017
SRR2727423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5001324
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT133350.2666293965357973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT132070.26407007424434015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT121730.24339554885866224No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG114710.22935926566645154No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC110120.22018169588692912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT89650.1792525339290156No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT67480.13492427205276042No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA65910.13178510330464493No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG57910.1157893389830373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC23950.024.4864313
CTTATAC25950.021.9576111
TTATACA26850.021.3594042
GCCGTCT9900.017.56565736
TACACAT33650.017.4829125
AAGACGG16600.017.2740975
CAAGACG17150.016.7201164
ATACACA37400.016.5213894
ACACATC37800.015.8082016
ATCTCCG36650.015.74897610
CGCAAGA17600.015.5568182
TATGCCG11600.015.15086233
GCGCAAG18500.015.01
ACATCTC39500.014.88
CGTACTA38300.014.73237529
CACATCT40800.014.6911767
ACCGTAC38800.014.63788727
ACGGACC18550.014.5606478
CGAACGA10400.014.40865416
TGCCGTC12150.014.31275735
Produced by FastQC (version 0.11.5)