FastQCFastQC Report
Thu 9 Feb 2017
SRR2727422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5160852
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT173680.3365335801142912No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT164320.31839703986861084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT162010.3139210347438756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117470.2276174554124009No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT72220.13993813424605084No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG70460.13652784462720496No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC69880.13540399918463075No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61010.11821691457146999No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG55920.10835420198060321No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT53650.10395570343811449No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC53000.1026962214766089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC27050.025.3733833
CTTATAC26800.024.3675371
TTATACA29400.022.653062
TACACAT34600.020.1040485
ATACACA37350.018.8714874
ACACATC40600.016.7684736
GCCGTCT8550.016.44444536
CGTACAC2150.016.3488373
AAGACGG18150.016.2066125
ACATCTC42350.016.1629288
TTCGCGA700.002594137815.85714322
CACATCT43650.015.554417
CAAGACG19300.015.5284974
ATCTCCG44200.015.10972910
ACGGACC19300.014.7616578
CGCAAGA19600.014.5357142
GCGAATC44950.014.52836533
GGCGAAT45100.014.43902532
TAAGGCG45950.014.41349329
CGCTTCG16050.014.408132
Produced by FastQC (version 0.11.5)