FastQCFastQC Report
Thu 9 Feb 2017
SRR2727421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382637
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56880.411387804608151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55250.3995987377742676No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC47760.34542689078912253No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG46880.3390622412100935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36990.26753225900941463No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA30750.22240110744902675No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG26550.1920243708218426No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22920.16577019130834775No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC19850.14356624334514412No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG19200.13886508172427037No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG18900.13669531482232863No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT18890.13662298925893057No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18660.13495950130077525No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT18480.1336576411596102No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG18110.1309815953138821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC4600.019.7065223
TATGCCG2300.018.533
GCCGTCT2600.017.07692336
TATACTG1655.4569682E-1116.818185
AAGACGG4300.016.779075
TTATACA5650.016.3716812
TACCGTG1251.658318E-716.2799997
CAAGACG4900.016.2346954
TGCCGTC2700.015.75925835
TACACAT6050.015.2892565
ATACACA6950.015.1726624
ATGCCGT2850.014.92982434
CGCTTCG3100.014.91935532
CTTCGGG3250.014.834
TAACGCC3500.014.84
TTCGGGC3000.014.835
ATCTCCG5350.014.52336410
CGCAAGA5000.014.4299992
CGCATCG3600.014.38888913
TCTATAC908.2755083E-414.3888893
Produced by FastQC (version 0.11.5)