##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727421.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1382637
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00789505850053	31.0	31.0	33.0	28.0	34.0
2	31.306742839950036	31.0	31.0	34.0	28.0	34.0
3	31.409126184240694	31.0	31.0	34.0	28.0	34.0
4	35.21586793930728	37.0	35.0	37.0	33.0	37.0
5	35.00052942312407	35.0	35.0	37.0	32.0	37.0
6	35.03224707569666	37.0	35.0	37.0	32.0	37.0
7	34.95425191138383	37.0	35.0	37.0	32.0	37.0
8	34.96005169831272	37.0	35.0	37.0	32.0	37.0
9	36.54324743226169	38.0	35.0	39.0	32.0	39.0
10	36.280460453466816	38.0	35.0	39.0	32.0	39.0
11	36.42286370175252	38.0	35.0	39.0	32.0	39.0
12	36.24776351276582	38.0	35.0	39.0	32.0	39.0
13	36.36638900882878	38.0	35.0	39.0	32.0	39.0
14	37.383132376755434	39.0	36.0	41.0	32.0	41.0
15	37.332508821910594	39.0	36.0	41.0	32.0	41.0
16	37.29549476833037	39.0	36.0	41.0	32.0	41.0
17	37.2521753721331	39.0	36.0	41.0	31.0	41.0
18	37.22600942980696	39.0	36.0	40.0	31.0	41.0
19	37.229268419693675	39.0	36.0	40.0	31.0	41.0
20	37.20587616272384	39.0	36.0	40.0	31.0	41.0
21	37.12614012210002	39.0	36.0	40.0	31.0	41.0
22	37.06649322996564	39.0	36.0	40.0	31.0	41.0
23	36.977604389293795	39.0	36.0	40.0	31.0	41.0
24	36.8947019355044	39.0	36.0	40.0	30.0	41.0
25	36.795525506694815	39.0	36.0	40.0	30.0	41.0
26	36.57661844721355	39.0	35.0	40.0	30.0	41.0
27	36.489159482930084	38.0	35.0	40.0	30.0	41.0
28	36.323467403230204	38.0	35.0	40.0	30.0	41.0
29	36.18480772610599	38.0	35.0	40.0	29.0	41.0
30	36.29385370129687	38.0	35.0	40.0	30.0	41.0
31	36.27498613157322	38.0	35.0	40.0	30.0	41.0
32	36.20667463694375	38.0	35.0	40.0	30.0	41.0
33	36.09719687813938	38.0	35.0	40.0	29.0	41.0
34	36.05950947356392	38.0	35.0	40.0	29.0	41.0
35	35.9648020413167	38.0	35.0	40.0	29.0	41.0
36	35.87224629458057	38.0	34.0	40.0	28.0	41.0
37	35.768003460054956	38.0	34.0	40.0	28.0	41.0
38	35.642827437715034	38.0	34.0	40.0	27.0	41.0
39	35.524669164791625	38.0	34.0	40.0	27.0	41.0
40	35.38623008063577	38.0	34.0	40.0	26.0	41.0
41	35.22179284946085	38.0	33.0	40.0	26.0	41.0
42	35.103478353320504	38.0	33.0	40.0	26.0	41.0
43	34.91653413007174	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	4.0
15	15.0
16	60.0
17	111.0
18	153.0
19	285.0
20	629.0
21	1171.0
22	2132.0
23	3648.0
24	5752.0
25	8849.0
26	12792.0
27	18380.0
28	24768.0
29	32690.0
30	41766.0
31	51733.0
32	63087.0
33	75949.0
34	92935.0
35	113330.0
36	140711.0
37	181673.0
38	263865.0
39	246145.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.070432803403925	20.776530643979584	12.89116376894297	25.261872783673518
2	19.194915223590865	23.21614422295946	32.55858189821334	25.030358655236334
3	21.508176043314332	24.72008198825867	29.34103455932396	24.43070740910304
4	16.109506689029732	17.188604094928746	33.01698131903023	33.68490789701129
5	14.826017240967804	37.07053984523776	33.37043634735654	14.7330065664379
6	34.571185350891085	34.70607252662846	15.544788690017697	15.17795343246275
7	27.86031329987553	31.111202723491417	21.623535316934237	19.404948659698825
8	27.756092163018923	33.19728894858159	20.269600770122597	18.777018118276885
9	26.80276891186913	13.844776322346359	19.5249367693762	39.82751799640831
10	17.502641691203113	26.959281431062525	32.27738010772169	23.260696770012665
11	36.12300263915981	21.818380384728602	20.49937908503823	21.559237891073362
12	21.402436069626376	26.557295949696126	29.005950224100758	23.034317756576744
13	30.85422999673812	19.218999636202415	24.69766106360527	25.229109303454194
14	22.881421515553253	21.100693819129678	24.881874273580124	31.136010391736953
15	26.339379027177777	27.057137918340104	21.998109409772777	24.60537364470935
16	25.107819333635657	25.513782720988953	25.016689123754105	24.361708821621296
17	24.19167142207246	26.81419635088603	25.291092311286334	23.703039915755184
18	24.936769376199248	23.786865243733534	26.916247720840687	24.360117659226535
19	24.641247124154788	26.004005389700986	26.314932986749234	23.039814499394996
20	24.498331810880224	24.359828356972944	26.491262710313695	24.650577121833134
21	24.995063780298082	25.470387382950115	25.93580238341662	23.598746453335185
22	24.692019669660223	25.46959180175274	25.65149059369885	24.186897934888187
23	23.357034420458877	24.687390833602745	26.51621502968603	25.43935971625235
24	24.022791231537997	26.540444093424377	25.859860541848654	23.57690413318897
25	24.742502912912066	25.071077947429444	25.174648154215458	25.011770985443032
26	24.613401782246534	25.749274755413026	25.957138424619043	23.680185037721397
27	25.218115817817694	25.03686795594216	25.297746263118952	24.447269963121197
28	24.156231896007412	25.13508607103672	26.844066808569423	23.864615224386444
29	24.08998167993479	25.173201642947497	26.53017386342185	24.206642813695858
30	23.643443651515184	26.17990115988506	26.83820843793418	23.338446750665575
31	25.281039057974002	25.289645800018373	25.097476778069733	24.331838363937898
32	23.377068601520136	25.755133126048268	25.824493341347004	25.043304931084588
33	23.88341987086994	25.277567430930898	26.415320868745738	24.42369182945343
34	24.249965826171294	25.539819923812253	26.101500249161564	24.10871400085489
35	24.491967161301194	25.771840331193218	26.91632004640408	22.819872461101504
36	24.278968377093914	25.903328205450887	25.70262476702128	24.115078650433915
37	24.21886583391013	25.82391473683982	26.132672566986127	23.824546862263922
38	24.016064954141978	25.274168129451187	26.627162443938644	24.082604472468187
39	24.66084735183566	24.48205855911566	25.75846010196458	25.098633987084103
40	24.478514606509155	25.51016644281905	26.6251373281635	23.386181622508296
41	23.49879252471907	25.525065508879045	26.449458534669624	24.526683431732263
42	25.06601515799158	25.847999149451372	26.312256940903506	22.773728751653543
43	24.51446041151799	24.410311600224787	26.343790886545058	24.731437101712164
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	125.0
1	170.0
2	215.0
3	474.0
4	733.0
5	733.0
6	1182.5
7	1632.0
8	1489.5
9	1347.0
10	1845.5
11	2344.0
12	2344.0
13	4277.5
14	6211.0
15	9296.0
16	12381.0
17	10935.0
18	9489.0
19	9489.0
20	11352.5
21	13216.0
22	11437.0
23	9658.0
24	11413.0
25	13168.0
26	13168.0
27	15758.5
28	18349.0
29	20675.5
30	23002.0
31	26142.5
32	29283.0
33	29283.0
34	34276.0
35	39269.0
36	46260.5
37	53252.0
38	59607.0
39	65962.0
40	65962.0
41	70687.0
42	75412.0
43	79338.0
44	83264.0
45	91895.5
46	100527.0
47	100527.0
48	119670.5
49	138814.0
50	136684.0
51	134554.0
52	119896.5
53	105239.0
54	105239.0
55	100623.0
56	96007.0
57	93852.5
58	91698.0
59	85388.5
60	79079.0
61	79079.0
62	66314.5
63	53550.0
64	47311.5
65	41073.0
66	35143.0
67	29213.0
68	29213.0
69	24400.5
70	19588.0
71	17375.0
72	15162.0
73	11295.5
74	7429.0
75	7429.0
76	5782.0
77	4135.0
78	3633.5
79	3132.0
80	2597.0
81	2062.0
82	2062.0
83	1721.5
84	1381.0
85	1211.0
86	1041.0
87	692.0
88	343.0
89	343.0
90	250.5
91	158.0
92	113.0
93	68.0
94	68.5
95	69.0
96	69.0
97	35.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1382637.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.50688736937087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.68074135431605	51.299272588799084
2	7.17892060799238	8.400325988909312
3	2.0541401864044624	3.6054404558059314
4	0.9196048800163212	2.1521287655775487
5	0.4940872089077836	1.4453752341107255
6	0.3295122829417928	1.1567242814939842
7	0.2182536068400845	0.8938537435346243
8	0.1639090682347009	0.7671847515220936
9	0.11566828144263719	0.6090652003115758
>10	0.6741241354826124	7.426457605328383
>50	0.07305326061604031	3.038070769554453
>100	0.08386340638836609	10.533494187611886
>500	0.009909979239920786	3.8302447463610423
>1k	0.003963991695968313	4.029696647853703
>5k	2.477494809980196E-4	0.8126650332257256
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5688	0.411387804608151	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5525	0.3995987377742676	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	4776	0.34542689078912253	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	4688	0.3390622412100935	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3699	0.26753225900941463	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	3075	0.22240110744902675	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	2655	0.1920243708218426	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2292	0.16577019130834775	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	1985	0.14356624334514412	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	1920	0.13886508172427037	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	1890	0.13669531482232863	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT	1889	0.13662298925893057	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	1866	0.13495950130077525	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1848	0.1336576411596102	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	1811	0.1309815953138821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.232556339805748E-5
2	0.0	0.0	0.0	7.232556339805748E-5	7.232556339805748E-5
3	0.0	0.0	0.0	7.232556339805748E-5	7.232556339805748E-5
4	0.0	0.0	0.0	7.232556339805748E-5	7.232556339805748E-5
5	0.0	0.0	0.0	7.232556339805748E-5	7.232556339805748E-5
6	0.0	0.0	0.0	1.4465112679611496E-4	7.232556339805748E-5
7	0.0	0.0	0.0	1.4465112679611496E-4	7.232556339805748E-5
8	0.0	0.0	0.0	1.4465112679611496E-4	7.232556339805748E-5
9	0.0	7.232556339805748E-5	0.0	2.1697669019417245E-4	7.232556339805748E-5
10	0.0	7.232556339805748E-5	0.0	2.1697669019417245E-4	7.232556339805748E-5
11	0.0	7.232556339805748E-5	0.0	2.1697669019417245E-4	7.232556339805748E-5
12	0.0	7.232556339805748E-5	0.0	2.1697669019417245E-4	7.232556339805748E-5
13	0.0	7.232556339805748E-5	0.0	2.1697669019417245E-4	7.232556339805748E-5
14	0.0	7.232556339805748E-5	0.0	2.1697669019417245E-4	7.232556339805748E-5
15	0.0	7.232556339805748E-5	0.0	2.1697669019417245E-4	7.232556339805748E-5
16	7.232556339805748E-5	7.232556339805748E-5	0.0	2.1697669019417245E-4	7.232556339805748E-5
17	7.232556339805748E-5	7.232556339805748E-5	0.0	2.893022535922299E-4	7.232556339805748E-5
18	7.232556339805748E-5	7.232556339805748E-5	0.0	3.616278169902874E-4	7.232556339805748E-5
19	7.232556339805748E-5	1.4465112679611496E-4	0.0	4.339533803883449E-4	7.232556339805748E-5
20	7.232556339805748E-5	1.4465112679611496E-4	0.0	5.062789437864023E-4	7.232556339805748E-5
21	7.232556339805748E-5	1.4465112679611496E-4	0.0	8.679067607766898E-4	7.232556339805748E-5
22	7.232556339805748E-5	1.4465112679611496E-4	0.0	0.0010848834509708622	7.232556339805748E-5
23	7.232556339805748E-5	1.4465112679611496E-4	0.0	0.0013018601411650347	1.4465112679611496E-4
24	7.232556339805748E-5	1.4465112679611496E-4	0.0	0.0022420924653397817	1.4465112679611496E-4
25	7.232556339805748E-5	1.4465112679611496E-4	0.0	0.002820696972524242	1.4465112679611496E-4
26	7.232556339805748E-5	1.4465112679611496E-4	0.0	0.0036886037333009316	1.4465112679611496E-4
27	7.232556339805748E-5	1.4465112679611496E-4	0.0	0.007521858593397978	1.4465112679611496E-4
28	7.232556339805748E-5	1.4465112679611496E-4	0.0	0.014392787116213439	1.4465112679611496E-4
29	7.232556339805748E-5	1.4465112679611496E-4	0.0	0.02582022613310652	1.4465112679611496E-4
30	7.232556339805748E-5	1.4465112679611496E-4	0.0	0.042816733531650025	1.4465112679611496E-4
31	7.232556339805748E-5	1.4465112679611496E-4	0.0	0.09214276776912524	1.4465112679611496E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	460	0.0	19.706522	3
TATGCCG	230	0.0	18.5	33
GCCGTCT	260	0.0	17.076923	36
TATACTG	165	5.4569682E-11	16.81818	5
AAGACGG	430	0.0	16.77907	5
TTATACA	565	0.0	16.371681	2
TACCGTG	125	1.658318E-7	16.279999	7
CAAGACG	490	0.0	16.234695	4
TGCCGTC	270	0.0	15.759258	35
TACACAT	605	0.0	15.289256	5
ATACACA	695	0.0	15.172662	4
ATGCCGT	285	0.0	14.929824	34
CGCTTCG	310	0.0	14.919355	32
CTTCGGG	325	0.0	14.8	34
TAACGCC	350	0.0	14.8	4
TTCGGGC	300	0.0	14.8	35
ATCTCCG	535	0.0	14.523364	10
CGCAAGA	500	0.0	14.429999	2
CGCATCG	360	0.0	14.388889	13
TCTATAC	90	8.2755083E-4	14.388889	3
>>END_MODULE