FastQCFastQC Report
Thu 9 Feb 2017
SRR2727420.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727420.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151012
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27920.24256914784554806No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG27400.23805138434699205No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC27300.23718258367419282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27250.23674818333779316No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18900.16420332715905656No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA16560.1438733914155543No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG15320.13310026307284373No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT15030.13058074112172594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTC1103.851528E-818.55
TATACAC2550.018.1372553
TACTATA1502.5102054E-1017.2666662
TACCGAC650.001579936417.0769217
GCGGGTA1554.0017767E-1016.70967923
ACCCGTT2250.015.62222230
AACCCGT2300.015.28260929
AAGACGG4050.015.0740745
CGGGTAA1601.096123E-815.03125124
TCTAGCG2109.094947E-1214.9761928
CGCTTCG2850.014.92982632
TACCGTC3100.014.9193557
GTAATAG750.004104410314.83
CGTAGAC750.004104410314.83
CAAGACG4150.014.7108434
CGAACGA2301.8189894E-1214.47826116
TAACGAA2301.8189894E-1214.47826113
ACGGACC4100.014.4390248
TTCGGGC2950.014.4237335
ATCTCCG2850.014.28070310
Produced by FastQC (version 0.11.5)