##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727420.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1151012
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.009629786657307	31.0	31.0	33.0	28.0	34.0
2	31.313403335499544	31.0	31.0	34.0	28.0	34.0
3	31.42579399693487	31.0	31.0	34.0	28.0	34.0
4	35.23348583681143	37.0	35.0	37.0	33.0	37.0
5	35.008046831831464	35.0	35.0	37.0	32.0	37.0
6	35.03796919580334	37.0	35.0	37.0	32.0	37.0
7	34.960919608136145	37.0	35.0	37.0	32.0	37.0
8	34.95370161214653	37.0	35.0	37.0	32.0	37.0
9	36.54730272143123	38.0	35.0	39.0	32.0	39.0
10	36.281981421566414	38.0	35.0	39.0	32.0	39.0
11	36.41899910687291	38.0	35.0	39.0	32.0	39.0
12	36.24492533527018	38.0	35.0	39.0	32.0	39.0
13	36.36187633143703	38.0	35.0	39.0	32.0	39.0
14	37.40780461020389	39.0	36.0	41.0	32.0	41.0
15	37.349812165294544	39.0	36.0	41.0	32.0	41.0
16	37.322106980639646	39.0	36.0	41.0	32.0	41.0
17	37.25557856912005	39.0	36.0	41.0	31.0	41.0
18	37.239355454156865	39.0	36.0	41.0	31.0	41.0
19	37.233515376034305	39.0	36.0	40.0	31.0	41.0
20	37.18478521509767	39.0	36.0	40.0	31.0	41.0
21	37.11491539619048	39.0	36.0	40.0	31.0	41.0
22	37.040759783564376	39.0	36.0	40.0	31.0	41.0
23	36.947633908247695	39.0	36.0	40.0	31.0	41.0
24	36.87822281609575	39.0	36.0	40.0	30.0	41.0
25	36.77467741431019	39.0	35.0	40.0	30.0	41.0
26	36.566449350658374	39.0	35.0	40.0	30.0	41.0
27	36.48361268170966	38.0	35.0	40.0	30.0	41.0
28	36.32881846583702	38.0	35.0	40.0	30.0	41.0
29	36.18884425184099	38.0	35.0	40.0	29.0	41.0
30	36.3063938516714	38.0	35.0	40.0	30.0	41.0
31	36.30124968288776	38.0	35.0	40.0	30.0	41.0
32	36.27039770219598	38.0	35.0	40.0	30.0	41.0
33	36.17053340886107	38.0	35.0	40.0	29.0	41.0
34	36.1343313536262	38.0	35.0	40.0	29.0	41.0
35	36.035906663006116	38.0	35.0	40.0	29.0	41.0
36	35.95351916400524	38.0	35.0	40.0	28.0	41.0
37	35.85713963016893	38.0	34.0	40.0	28.0	41.0
38	35.743841940831196	38.0	34.0	40.0	27.0	41.0
39	35.648439807751785	38.0	34.0	40.0	27.0	41.0
40	35.51425267503727	38.0	34.0	40.0	27.0	41.0
41	35.36084506503842	38.0	34.0	40.0	26.0	41.0
42	35.250694171737564	38.0	33.0	40.0	26.0	41.0
43	35.09697813749987	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	6.0
15	10.0
16	46.0
17	67.0
18	125.0
19	256.0
20	474.0
21	943.0
22	1727.0
23	2865.0
24	4625.0
25	7333.0
26	10556.0
27	15041.0
28	20189.0
29	27160.0
30	34719.0
31	42803.0
32	52027.0
33	63248.0
34	76715.0
35	93487.0
36	115572.0
37	150260.0
38	220793.0
39	209962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.81286728548443	20.601696593953843	12.879188053643228	25.7062480669185
2	19.70092405639559	22.588817492780265	32.581849711384415	25.128408739439728
3	21.38118455758932	24.24822677782682	29.0047367012681	25.365851963315762
4	16.229457208091663	16.89817308594524	33.31042595559386	33.56194375036924
5	15.2248629901339	36.46061031509662	33.40095498569954	14.913571709069931
6	35.053240105229136	34.80206983072288	15.36265477683986	14.782035287208126
7	28.69857134417365	30.602634898680463	21.454858854642698	19.243934902503188
8	27.50405729914197	33.42484700420152	19.93089559448555	19.140200102170958
9	26.823699492272883	13.927743585644633	19.31265703572161	39.93589988636087
10	17.436395102744367	26.45185280431481	32.44414480474574	23.667607288195082
11	36.84670533408861	21.54130452158622	20.521593171921754	21.090396972403415
12	21.65529116985748	25.830225922926953	28.916466552911697	23.598016354303862
13	30.784822399766465	19.25175410855838	24.448659093041602	25.51476439863355
14	23.01783126500853	20.631322696896294	24.341449090018173	32.009396948077
15	26.069232988014022	27.28955041302784	21.562155737733406	25.079060861224733
16	25.566631798799666	25.558812592744474	24.230677004236274	24.64387860421959
17	24.51668618572178	26.3770490663868	24.71329577797625	24.39296896991517
18	25.178191017991125	24.01834211980414	26.111977981115746	24.69148888108899
19	25.067679572411063	25.61789103849482	25.464200199476632	23.850229189617483
20	25.07914774129201	24.342231010623692	25.450907549182805	25.127713698901488
21	25.53431241377153	25.122935295201092	25.21433312597957	24.128419165047802
22	25.251344034640823	24.954996125149	25.186792144651836	24.606867695558343
23	24.062216553780498	24.57637279194309	25.74143449416687	25.619976160109537
24	24.707040413132095	25.848818257324858	25.258468200157775	24.185673129385272
25	25.18375134229704	24.79244351926826	24.70565033205562	25.318154806379084
26	25.018418574263347	25.557161871466157	25.33023113573099	24.09418841853951
27	25.602426386518996	24.7455282829371	24.790532157788103	24.8615131727558
28	24.64830948765087	25.01164192901551	25.987565724770896	24.352482858562727
29	24.654477972427742	24.79305167973922	25.8239705580828	24.728499789750238
30	24.217992514413403	25.657421469107184	26.037956163793254	24.08662985268616
31	25.433184015457698	25.057253964337473	24.679586311871642	24.829975708333187
32	24.09688170062519	25.16481148763002	25.10999016517638	25.62831664656841
33	24.378981279083103	24.947611319430205	25.750296261029426	24.923111140457266
34	25.10929512463814	24.92319802052455	25.44717170628977	24.520335148547538
35	25.124586016479412	25.157339801843943	25.93813096648862	23.779943215188027
36	24.468293988246863	25.50659767230924	25.33466201916227	24.69044632028163
37	24.939705233307734	25.196435832119906	25.48183685313446	24.3820220814379
38	24.62007346578489	24.671940865951004	26.014498545627674	24.69348712263643
39	25.211726723961174	24.250659419710654	25.16020684406418	25.37740701226399
40	24.902259924310087	25.089312709163764	26.00520237842872	24.00322498809743
41	24.133892609286438	24.993744635155846	26.098077170350958	24.774285585206755
42	25.545172422181523	25.284619100409035	25.73300712764072	23.437201349768724
43	24.910600410768957	24.167775835525607	25.83622064756927	25.085403106136166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	89.0
1	100.5
2	112.0
3	250.5
4	389.0
5	389.0
6	612.5
7	836.0
8	784.0
9	732.0
10	994.5
11	1257.0
12	1257.0
13	2257.5
14	3258.0
15	4844.5
16	6431.0
17	5916.0
18	5401.0
19	5401.0
20	6444.5
21	7488.0
22	6989.5
23	6491.0
24	7984.5
25	9478.0
26	9478.0
27	11608.5
28	13739.0
29	15646.0
30	17553.0
31	20203.5
32	22854.0
33	22854.0
34	27382.5
35	31911.0
36	37862.0
37	43813.0
38	49315.0
39	54817.0
40	54817.0
41	58915.0
42	63013.0
43	66867.5
44	70722.0
45	77889.0
46	85056.0
47	85056.0
48	97234.5
49	109413.0
50	109154.5
51	108896.0
52	100602.5
53	92309.0
54	92309.0
55	88905.5
56	85502.0
57	83419.5
58	81337.0
59	75194.5
60	69052.0
61	69052.0
62	59065.0
63	49078.0
64	43205.0
65	37332.0
66	31687.0
67	26042.0
68	26042.0
69	21578.0
70	17114.0
71	14943.5
72	12773.0
73	9544.0
74	6315.0
75	6315.0
76	5007.0
77	3699.0
78	3128.0
79	2557.0
80	2092.5
81	1628.0
82	1628.0
83	1404.5
84	1181.0
85	1011.5
86	842.0
87	554.5
88	267.0
89	267.0
90	195.0
91	123.0
92	87.0
93	51.0
94	51.5
95	52.0
96	52.0
97	28.5
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1151012.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.577949218444175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.40977667789384	53.82514789466892
2	7.465402083766724	9.194083008189093
3	2.08479161323831	3.851315762730809
4	0.9307493442552809	2.292545434226076
5	0.49809181235365274	1.5335736163618023
6	0.322601457911757	1.1919081715851736
7	0.21250100770967725	0.9159763383130305
8	0.15092768957205593	0.7435054083300131
9	0.11411827157796811	0.6324452218912266
>10	0.6529158846754091	7.4972204575618315
>50	0.07158500788371235	3.1130515525037277
>100	0.07805492138252927	10.312732679271715
>500	0.006504574630628095	2.816307156382889
>1k	0.0019796531484520285	2.080187297983775
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2792	0.24256914784554806	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	2740	0.23805138434699205	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	2730	0.23718258367419282	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2725	0.23674818333779316	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1890	0.16420332715905656	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	1656	0.1438733914155543	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	1532	0.13310026307284373	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1503	0.13058074112172594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.68800672799241E-5	0.0
5	0.0	0.0	0.0	8.68800672799241E-5	0.0
6	0.0	0.0	0.0	8.68800672799241E-5	0.0
7	0.0	0.0	0.0	8.68800672799241E-5	0.0
8	0.0	0.0	0.0	8.68800672799241E-5	8.68800672799241E-5
9	0.0	8.68800672799241E-5	0.0	8.68800672799241E-5	8.68800672799241E-5
10	0.0	8.68800672799241E-5	0.0	8.68800672799241E-5	8.68800672799241E-5
11	0.0	8.68800672799241E-5	0.0	8.68800672799241E-5	8.68800672799241E-5
12	0.0	8.68800672799241E-5	0.0	8.68800672799241E-5	8.68800672799241E-5
13	0.0	8.68800672799241E-5	0.0	8.68800672799241E-5	8.68800672799241E-5
14	0.0	1.737601345598482E-4	0.0	8.68800672799241E-5	8.68800672799241E-5
15	0.0	1.737601345598482E-4	0.0	8.68800672799241E-5	8.68800672799241E-5
16	0.0	1.737601345598482E-4	0.0	8.68800672799241E-5	8.68800672799241E-5
17	0.0	1.737601345598482E-4	0.0	1.737601345598482E-4	8.68800672799241E-5
18	0.0	1.737601345598482E-4	0.0	1.737601345598482E-4	8.68800672799241E-5
19	0.0	1.737601345598482E-4	0.0	2.606402018397723E-4	2.606402018397723E-4
20	0.0	1.737601345598482E-4	0.0	4.344003363996205E-4	2.606402018397723E-4
21	0.0	1.737601345598482E-4	0.0	6.950405382393928E-4	2.606402018397723E-4
22	0.0	2.606402018397723E-4	0.0	9.556807400791651E-4	2.606402018397723E-4
23	0.0	2.606402018397723E-4	0.0	0.0011294408746390133	2.606402018397723E-4
24	0.0	2.606402018397723E-4	0.0	0.0018244814128784061	2.606402018397723E-4
25	0.0	2.606402018397723E-4	0.0	0.0023457618165579506	2.606402018397723E-4
26	0.0	2.606402018397723E-4	0.0	0.0028670422202374955	2.606402018397723E-4
27	0.0	2.606402018397723E-4	0.0	0.004952163834955674	2.606402018397723E-4
28	0.0	2.606402018397723E-4	0.0	0.011294408746390133	2.606402018397723E-4
29	0.0	2.606402018397723E-4	0.0	0.019895535407102618	2.606402018397723E-4
30	0.0	2.606402018397723E-4	0.0	0.03761906913220714	2.606402018397723E-4
31	0.0	2.606402018397723E-4	0.0	0.08844390849096273	2.606402018397723E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACTC	110	3.851528E-8	18.5	5
TATACAC	255	0.0	18.137255	3
TACTATA	150	2.5102054E-10	17.266666	2
TACCGAC	65	0.0015799364	17.076921	7
GCGGGTA	155	4.0017767E-10	16.709679	23
ACCCGTT	225	0.0	15.622222	30
AACCCGT	230	0.0	15.282609	29
AAGACGG	405	0.0	15.074074	5
CGGGTAA	160	1.096123E-8	15.031251	24
TCTAGCG	210	9.094947E-12	14.97619	28
CGCTTCG	285	0.0	14.929826	32
TACCGTC	310	0.0	14.919355	7
GTAATAG	75	0.0041044103	14.8	3
CGTAGAC	75	0.0041044103	14.8	3
CAAGACG	415	0.0	14.710843	4
CGAACGA	230	1.8189894E-12	14.478261	16
TAACGAA	230	1.8189894E-12	14.478261	13
ACGGACC	410	0.0	14.439024	8
TTCGGGC	295	0.0	14.42373	35
ATCTCCG	285	0.0	14.280703	10
>>END_MODULE