FastQCFastQC Report
Thu 9 Feb 2017
SRR2727419.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727419.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424595
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC144530.5960995547710031No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG141440.583355158284167No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA90820.37457802230888043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76050.31366063198183614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75490.31135096789360694No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG74860.30875259579434916No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC56530.23315234090641943No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG55800.2301415287914064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52010.214510052194284No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT51340.21174670408872412No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG49590.20452900381300793No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG48800.20127072768854182No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC39560.16316127023276053No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34980.14427151751117195No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC34210.14109572938985687No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC33480.13808491727484384No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA33370.1376312332575131No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30340.12513430078013027No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT29920.1234020527139584No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC29210.12047372860209643No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC29200.12043248460052092No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT28980.11952511656585946No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT25790.1063682800632683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG8450.015.5443785
ACGGACC8450.015.1065088
CGCTTCG5850.014.86324732
ACGCTTC5950.014.61344631
CAAGACG8550.014.4970764
ACGAACG4750.014.41052615
CGAACGA4900.014.3469416
ATACCGT7500.014.3066666
TAACGAA4950.014.20202113
GTAAACG5750.014.15652227
CGCAAGA8800.014.0852272
GTCTAAT1458.9209243E-714.0344841
ACTATAC1854.9058144E-914.03
TATACCG800.006301279613.8755
AAACGCT6050.013.7603329
CTAGATA4850.013.7319583
GCGCAAG9050.013.6961331
TTCGGGC6400.013.585937535
TAAACGC6050.013.45454528
AGACGGA10000.013.326
Produced by FastQC (version 0.11.5)