FastQCFastQC Report
Thu 9 Feb 2017
SRR2727418.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727418.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306293
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32510.24887219023603432No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31100.23807828718365637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29160.22322710142364693No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG27490.210442833269412No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT24010.1838025619060961No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA23710.18150598678856888No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG19990.1530284553312312No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG17720.13565103694194183No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC15020.11498186088419672No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG14430.11046526315305985No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC14090.10786247801986232No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14070.10770937301202717No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT13900.10640798044542839No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG13830.10587211291800537No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGG13490.10326932778480784No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG13390.10250380274563212No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13350.1021975927299618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAA358.8700466E-426.4285733
ATCTACG500.00703538618.49999828
CAAGACG5700.017.8508764
AAGACGG6000.017.5755
GTAAGAC852.7235757E-517.4117643
CGCAAGA6250.016.282
GTAGCAC957.060854E-515.5789483
AGGTTAT3100.015.51612951
ACGGACC6450.015.4883728
CGGACCA6450.015.4883729
ACCGTCG4200.015.4166668
ACGAACG2300.015.28260915
TATACTG1954.1836756E-1115.1794875
GCGCAAG6800.014.9632361
GACGGAC6800.014.9632367
TACCGTC4100.014.89024457
GCAAGAC7450.014.4026843
TAACCTA908.275082E-414.3888885
ATACCGT4900.014.3469386
CGTCGTA4400.014.29545410
Produced by FastQC (version 0.11.5)