##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727418.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1306293
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.606790360202496	31.0	30.0	31.0	27.0	34.0
2	30.908838981759835	31.0	31.0	34.0	27.0	34.0
3	30.975746635708834	31.0	31.0	34.0	27.0	34.0
4	34.804858481213635	35.0	35.0	37.0	32.0	37.0
5	34.544455187312494	35.0	35.0	37.0	30.0	37.0
6	34.68447813775317	35.0	35.0	37.0	31.0	37.0
7	34.63289399851335	35.0	35.0	37.0	30.0	37.0
8	34.63667339563176	35.0	35.0	37.0	30.0	37.0
9	36.18595062516602	38.0	35.0	39.0	32.0	39.0
10	35.857206614442546	37.0	35.0	39.0	30.0	39.0
11	36.053211645473105	37.0	35.0	39.0	31.0	39.0
12	35.8311381902835	37.0	35.0	39.0	30.0	39.0
13	35.987779923799636	37.0	35.0	39.0	30.0	39.0
14	36.924081350814866	38.0	36.0	40.0	31.0	41.0
15	36.88118362419457	38.0	36.0	40.0	31.0	41.0
16	36.83898252535993	38.0	36.0	40.0	31.0	41.0
17	36.756901399609426	38.0	36.0	40.0	31.0	41.0
18	36.7337312532487	38.0	36.0	40.0	31.0	41.0
19	36.7206683339802	38.0	35.0	40.0	30.0	41.0
20	36.6757557454568	38.0	35.0	40.0	30.0	41.0
21	36.5839746519349	38.0	35.0	40.0	30.0	41.0
22	36.5194079735557	38.0	35.0	40.0	30.0	41.0
23	36.41220537811961	38.0	35.0	40.0	30.0	41.0
24	36.32314878821214	38.0	35.0	40.0	30.0	41.0
25	36.21123897931015	38.0	34.0	40.0	29.0	41.0
26	35.97147653704031	38.0	34.0	40.0	29.0	41.0
27	35.878722461193625	38.0	34.0	40.0	28.0	41.0
28	35.69426460985399	38.0	34.0	40.0	27.0	41.0
29	35.53387716232116	38.0	34.0	40.0	27.0	41.0
30	35.66358083523375	38.0	34.0	40.0	27.0	41.0
31	35.66732042505012	38.0	34.0	40.0	27.0	41.0
32	35.622395588126096	38.0	34.0	40.0	27.0	41.0
33	35.510004263974466	38.0	34.0	40.0	27.0	41.0
34	35.47192475195075	38.0	34.0	40.0	27.0	41.0
35	35.35274628280179	38.0	34.0	40.0	27.0	41.0
36	35.25228413533564	38.0	34.0	40.0	26.0	41.0
37	35.16779390228685	38.0	33.0	40.0	26.0	41.0
38	35.05508335419389	38.0	33.0	40.0	26.0	41.0
39	34.95112046072359	38.0	33.0	40.0	25.0	41.0
40	34.801467970815125	38.0	33.0	40.0	25.0	41.0
41	34.621855127448434	37.0	33.0	40.0	24.0	41.0
42	34.49791662360588	37.0	33.0	40.0	24.0	41.0
43	34.331100296794055	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	9.0
13	7.0
14	21.0
15	43.0
16	93.0
17	169.0
18	247.0
19	493.0
20	904.0
21	1672.0
22	2934.0
23	4599.0
24	7219.0
25	10570.0
26	15242.0
27	20837.0
28	27886.0
29	36050.0
30	45016.0
31	55205.0
32	67069.0
33	80183.0
34	96631.0
35	117168.0
36	147186.0
37	191626.0
38	252488.0
39	124724.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.713831429855325	22.8798592658768	13.776082394991015	23.63022690927686
2	22.868912257816586	23.763964133620867	30.05550822059063	23.31161538797192
3	24.465567832025435	24.29271227817955	27.950697125376927	23.29102276441809
4	19.50320487057651	17.97131271468193	31.285706958546054	31.239775456195506
5	15.623600524537757	37.34070380841052	32.77511247476638	14.260583192285345
6	35.458890157108705	35.29039809598613	14.693640706947065	14.55707103995811
7	28.91625385728929	30.91894391227695	21.137447724208887	19.027354506224867
8	27.84130359727871	33.68463277381108	19.600273445543994	18.87379018336621
9	27.02594287805263	13.699529891073443	18.87325431583879	40.40127291503514
10	17.610367658710565	26.7422392985341	32.0455671124319	23.601825930323443
11	37.702108179405386	21.222497556061313	19.957467428823396	21.117926835709905
12	21.64169906751395	26.23400722502532	28.604302403825177	23.519991303635553
13	31.488953856447218	18.947586797142755	24.14159763544626	25.42186171096377
14	23.197399052126897	20.58787729858462	24.049504973233418	32.16521867605507
15	26.553920138896864	27.465966670570847	20.998275272086737	24.981837918445553
16	26.067275871492846	25.599693177564298	23.733419684557752	24.599611266385107
17	24.792523576257395	26.635678213080833	24.228561279896624	24.34323693076515
18	25.600458702603472	23.736634889722293	25.973039739170307	24.689866668503928
19	25.56899562349335	25.665145568413823	24.977857188241842	23.78800161985098
20	25.504155652675166	24.052949835909708	25.078064415869942	25.364830095545184
21	26.064596533855728	25.268450493112955	24.431195757766446	24.235757215264876
22	25.63620872193298	25.281770628794614	24.60558236169068	24.47643828758173
23	24.405627221457973	24.30036752857131	25.615462993371317	25.6785422565994
24	24.996153236678143	25.853541280554975	25.05280208957715	24.097503393189736
25	25.53868083194199	24.69698605136826	24.340863803143705	25.423469313546043
26	25.346840257124548	25.479735403925456	24.907046122118086	24.266378216831903
27	26.171081066805073	24.51272417443866	24.447654546108723	24.868540212647545
28	25.32157793083175	24.647686238845342	25.75149679283285	24.279239037490058
29	25.030372205929297	24.670269227501027	25.574048088751912	24.725310477817764
30	24.803547136821525	25.310630922771537	25.52206893859188	24.363753001815063
31	26.087715390038834	25.052419327057557	24.220370161977442	24.639495120926163
32	24.67991484299464	24.935753311087176	24.780734490654087	25.603597355264096
33	24.807604419529156	24.686498358331555	25.522528253615384	24.983368968523905
34	25.633376279288033	24.618596287356663	25.221064493187978	24.52696294016733
35	25.79183996239741	25.052342774553644	25.58989445706285	23.5659228059861
36	25.006870587226604	25.603444250256256	24.75355835176335	24.63612681075379
37	25.54028843452426	25.08150927854624	24.99439252908804	24.383809757841465
38	25.08281067111284	24.406852061520652	25.7316696943182	24.778667573048313
39	25.802404207938036	24.106995903675514	24.703263356689504	25.387336531696945
40	25.511428140547338	24.944939611557285	25.418876163310987	24.12475608458439
41	24.665599524762055	24.872674047859096	25.5548334102686	24.906893017110253
42	26.262178546466984	25.266919443034602	25.13379463872194	23.33710737177647
43	25.723478576399017	24.07974321228086	25.44926750736626	24.74751070395386
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	118.0
1	132.5
2	147.0
3	250.5
4	354.0
5	354.0
6	598.0
7	842.0
8	786.0
9	730.0
10	1021.0
11	1312.0
12	1312.0
13	2540.0
14	3768.0
15	5262.5
16	6757.0
17	5992.5
18	5228.0
19	5228.0
20	6731.0
21	8234.0
22	7656.0
23	7078.0
24	8580.5
25	10083.0
26	10083.0
27	12246.5
28	14410.0
29	16333.0
30	18256.0
31	21218.0
32	24180.0
33	24180.0
34	29310.5
35	34441.0
36	41353.0
37	48265.0
38	54284.5
39	60304.0
40	60304.0
41	64975.5
42	69647.0
43	73878.5
44	78110.0
45	86811.5
46	95513.0
47	95513.0
48	110682.0
49	125851.0
50	125104.0
51	124357.0
52	114532.5
53	104708.0
54	104708.0
55	101516.5
56	98325.0
57	96105.0
58	93885.0
59	88248.5
60	82612.0
61	82612.0
62	69295.0
63	55978.0
64	49721.0
65	43464.0
66	37020.5
67	30577.0
68	30577.0
69	25544.0
70	20511.0
71	18452.5
72	16394.0
73	12280.5
74	8167.0
75	8167.0
76	6403.5
77	4640.0
78	3995.0
79	3350.0
80	2785.5
81	2221.0
82	2221.0
83	1838.0
84	1455.0
85	1329.0
86	1203.0
87	809.0
88	415.0
89	415.0
90	309.0
91	203.0
92	152.0
93	101.0
94	89.0
95	77.0
96	77.0
97	43.0
98	9.0
99	11.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1306293.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.150362304606006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.01307906491787	52.94018593298745
2	7.044971187560812	8.475151387145864
3	1.9935791336144986	3.597435215094439
4	0.903188431618463	2.1730844556471767
5	0.5066880776122875	1.5238735721901713
6	0.30273870927466706	1.0925905827900007
7	0.20555149593321173	0.8654797866845504
8	0.15555687544900618	0.7485441933784155
9	0.10880665571999126	0.5890283786439006
>10	0.6005609540965523	6.697830472343412
>50	0.0687767915484956	2.9452400487700503
>100	0.08400956132269301	11.250775270131431
>500	0.008923615236798077	3.6136895375583697
>1k	0.0035694460947192316	3.487091166634931
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3251	0.24887219023603432	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	3110	0.23807828718365637	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2916	0.22322710142364693	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	2749	0.210442833269412	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2401	0.1838025619060961	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	2371	0.18150598678856888	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	1999	0.1530284553312312	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG	1772	0.13565103694194183	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	1502	0.11498186088419672	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG	1443	0.11046526315305985	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1409	0.10786247801986232	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1407	0.10770937301202717	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	1390	0.10640798044542839	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	1383	0.10587211291800537	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGG	1349	0.10326932778480784	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	1339	0.10250380274563212	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1335	0.1021975927299618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.655250391757438E-5	0.0
6	0.0	0.0	0.0	7.655250391757438E-5	0.0
7	0.0	0.0	0.0	7.655250391757438E-5	0.0
8	0.0	0.0	0.0	7.655250391757438E-5	0.0
9	0.0	0.0	0.0	1.5310500783514877E-4	0.0
10	0.0	0.0	0.0	1.5310500783514877E-4	0.0
11	0.0	0.0	0.0	2.2965751175272315E-4	0.0
12	0.0	0.0	0.0	2.2965751175272315E-4	0.0
13	0.0	0.0	0.0	3.0621001567029754E-4	7.655250391757438E-5
14	0.0	0.0	0.0	3.0621001567029754E-4	1.5310500783514877E-4
15	0.0	0.0	0.0	3.0621001567029754E-4	1.5310500783514877E-4
16	0.0	0.0	0.0	3.8276251958787195E-4	1.5310500783514877E-4
17	0.0	0.0	0.0	4.593150235054463E-4	1.5310500783514877E-4
18	0.0	0.0	0.0	4.593150235054463E-4	1.5310500783514877E-4
19	0.0	0.0	0.0	6.889725352581695E-4	1.5310500783514877E-4
20	0.0	0.0	0.0	7.655250391757439E-4	1.5310500783514877E-4
21	0.0	0.0	0.0	7.655250391757439E-4	1.5310500783514877E-4
22	0.0	0.0	0.0	0.0010717350548460414	1.5310500783514877E-4
23	0.0	0.0	0.0	0.0012248400626811901	2.2965751175272315E-4
24	0.0	0.0	0.0	0.001760707590104211	2.2965751175272315E-4
25	0.0	0.0	0.0	0.0019138125979393597	2.2965751175272315E-4
26	0.0	0.0	0.0	0.0026793376371151037	2.2965751175272315E-4
27	0.0	0.0	0.0	0.0048228077468071865	2.2965751175272315E-4
28	0.0	0.0	0.0	0.010870455556295563	2.2965751175272315E-4
29	0.0	0.0	0.0	0.017147760877536663	2.2965751175272315E-4
30	0.0	0.0	0.0	0.029549266512183713	2.2965751175272315E-4
31	0.0	0.0	0.0	0.06445720829859763	2.2965751175272315E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTAA	35	8.8700466E-4	26.42857	33
ATCTACG	50	0.007035386	18.499998	28
CAAGACG	570	0.0	17.850876	4
AAGACGG	600	0.0	17.575	5
GTAAGAC	85	2.7235757E-5	17.411764	3
CGCAAGA	625	0.0	16.28	2
GTAGCAC	95	7.060854E-5	15.578948	3
AGGTTAT	310	0.0	15.5161295	1
ACGGACC	645	0.0	15.488372	8
CGGACCA	645	0.0	15.488372	9
ACCGTCG	420	0.0	15.416666	8
ACGAACG	230	0.0	15.282609	15
TATACTG	195	4.1836756E-11	15.179487	5
GCGCAAG	680	0.0	14.963236	1
GACGGAC	680	0.0	14.963236	7
TACCGTC	410	0.0	14.8902445	7
GCAAGAC	745	0.0	14.402684	3
TAACCTA	90	8.275082E-4	14.388888	5
ATACCGT	490	0.0	14.346938	6
CGTCGTA	440	0.0	14.295454	10
>>END_MODULE