FastQCFastQC Report
Thu 9 Feb 2017
SRR2727417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776260
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT287991.6213279587447782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT103900.5849368898697263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103160.5807708330987581No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71930.4049519777510049No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42080.2369022553004628No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41810.2353822075597041No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA27800.15650861923367074No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26490.14913357278776757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG2500.016.2799995
TAGATCG803.3838657E-416.18755
TATACCG803.3838657E-416.18755
AACGCAG121100.015.7960375
CGCACTA750.004105577214.829
AGCGCGT1001.0938973E-414.79999932
TTACACT3150.014.682544
GTATTAG2400.014.6458341
CGCAATC908.277147E-414.38888822
TCGGTAT908.277147E-414.38888827
ACGCAGA133900.014.3413016
CGCAGAG135550.014.1940247
ACGCTAT800.006300406513.87511
CCCGCGA800.006300406513.87521
GTGTACG950.001245541313.6315791
AGAGTAC141250.013.58194710
TAGCGAC1102.4575423E-413.45454524
ATTGGAC1652.5970803E-713.4545453
CAGAGTA143800.013.4182899
GCAGAGT144150.013.321548
Produced by FastQC (version 0.11.5)