##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727417.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1776260
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.299417315032706	33.0	31.0	34.0	30.0	34.0
2	32.42132852172542	34.0	31.0	34.0	31.0	34.0
3	32.50807257946472	34.0	31.0	34.0	31.0	34.0
4	36.044124733991644	37.0	35.0	37.0	35.0	37.0
5	35.86186256516501	37.0	35.0	37.0	35.0	37.0
6	35.89566898990013	37.0	35.0	37.0	35.0	37.0
7	35.861600779165215	37.0	35.0	37.0	35.0	37.0
8	35.82634749417315	37.0	35.0	37.0	35.0	37.0
9	37.64804589418216	39.0	37.0	39.0	35.0	39.0
10	37.48652787317172	39.0	37.0	39.0	35.0	39.0
11	37.58963214844674	39.0	37.0	39.0	35.0	39.0
12	37.52545460687062	39.0	37.0	39.0	35.0	39.0
13	37.6073322599169	39.0	37.0	39.0	35.0	39.0
14	38.776733698895434	40.0	38.0	41.0	35.0	41.0
15	38.70840755294833	40.0	38.0	41.0	35.0	41.0
16	38.69288842849583	40.0	38.0	41.0	35.0	41.0
17	38.658802765360925	40.0	38.0	41.0	35.0	41.0
18	38.65325853197167	40.0	38.0	41.0	35.0	41.0
19	38.70837546305158	40.0	38.0	41.0	34.0	41.0
20	38.664519833808114	40.0	38.0	41.0	34.0	41.0
21	38.60819474626462	40.0	38.0	41.0	34.0	41.0
22	38.55003997162578	40.0	38.0	41.0	34.0	41.0
23	38.46591152196188	40.0	38.0	41.0	34.0	41.0
24	38.41547915282673	40.0	38.0	41.0	34.0	41.0
25	38.29745814238906	40.0	38.0	41.0	34.0	41.0
26	38.167503068244514	40.0	37.0	41.0	34.0	41.0
27	38.059493542612	40.0	37.0	41.0	33.0	41.0
28	37.94586265524191	40.0	37.0	41.0	33.0	41.0
29	37.82791708421065	40.0	37.0	41.0	33.0	41.0
30	37.716056770968216	40.0	37.0	41.0	33.0	41.0
31	37.568224246450406	40.0	36.0	41.0	33.0	41.0
32	37.418088005134386	39.0	36.0	41.0	33.0	41.0
33	37.264982603898076	39.0	36.0	41.0	32.0	41.0
34	37.11744789614133	39.0	35.0	41.0	32.0	41.0
35	36.98230945920079	39.0	35.0	41.0	31.0	41.0
36	36.83769718397082	39.0	35.0	41.0	31.0	41.0
37	36.693336561089026	39.0	35.0	41.0	31.0	41.0
38	36.542377242070415	39.0	35.0	41.0	30.0	41.0
39	36.3892251134406	39.0	35.0	41.0	30.0	41.0
40	36.21350872057019	39.0	35.0	40.0	30.0	41.0
41	36.04970612410345	39.0	35.0	40.0	29.0	41.0
42	35.8660477632779	39.0	35.0	40.0	27.0	41.0
43	34.29219371038024	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	14.0
11	12.0
12	14.0
13	11.0
14	6.0
15	11.0
16	16.0
17	26.0
18	46.0
19	124.0
20	274.0
21	537.0
22	988.0
23	1747.0
24	3002.0
25	5075.0
26	7695.0
27	11582.0
28	16662.0
29	23084.0
30	29538.0
31	36680.0
32	45232.0
33	58404.0
34	77392.0
35	105221.0
36	156448.0
37	263629.0
38	458683.0
39	474100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.09624717102226	18.564343057885672	14.322340197943994	23.017069573148074
2	16.118417348811548	23.365836082555482	37.18323894024523	23.332507628387734
3	18.291072252935944	24.850247148503037	31.557598549761863	25.301082048799163
4	12.897605080337337	15.896546676725254	35.679405042054654	35.52644320088275
5	14.078231790391046	38.28020672649274	34.20743584835553	13.434125634760678
6	32.9907783770394	37.040579644871805	15.389582606150002	14.579059371938794
7	27.847499802956776	31.201738484230912	22.663292536002615	18.2874691768097
8	27.36423721752446	35.84582212063549	20.289766137840182	16.500174523999863
9	25.946201569590038	14.18745003546778	19.982435003884564	39.88391339105761
10	14.95726976906534	28.627171697837028	34.47845473072636	21.937103802371276
11	34.665026516388366	22.178003220249288	23.086372490513778	20.07059777284857
12	20.373988042291106	27.028757051332576	32.01918638037225	20.578068526004074
13	31.2575298661232	19.705110738292817	26.169704885546036	22.867654510037944
14	20.86152928062333	20.513832434440904	29.867755846553994	28.75688243838177
15	23.982862869174557	29.265479152826725	24.161834416132773	22.589823561865945
16	22.294878002094286	26.700257845135283	26.519428462049476	24.48543569072095
17	22.50897954128337	27.36592615945864	28.365160505781812	21.75993379347618
18	22.479197865177394	25.95048022248995	29.18958936191774	22.380732550414915
19	23.574476709490728	26.47613525047009	29.26784367153457	20.68154436850461
20	22.51494713611746	26.966998074606195	28.948915136297614	21.56913965297873
21	21.927758323668833	27.460450609708037	29.00898517108982	21.60280589553331
22	22.150698658980104	26.903719050139056	28.83260333509734	22.1129789557835
23	21.57049080652607	26.643453098082485	29.16211590645514	22.623940188936302
24	21.83481021922466	26.877821940481688	29.19916003287807	22.08820780741558
25	22.180761825408442	26.7955704682873	29.046986364608784	21.97668134169547
26	21.88637924628151	27.144731064146015	29.53903144809881	21.42985824147366
27	22.26104286534629	27.396833796854065	29.11150394649432	21.23061939130533
28	21.885985159830206	27.4607321000304	29.167914607095806	21.485368133043586
29	21.596162723925552	27.52975352707374	29.360341391462963	21.513742357537748
30	21.368718543456474	27.679900465022012	29.3594406224314	21.59194036909011
31	21.590420321349352	26.92646346818596	29.45480954364789	22.028306666816793
32	21.193913053269227	27.423237589091688	29.28417011023161	22.098679247407475
33	21.618287863263262	27.077454877101324	29.136387690991185	22.16786956864423
34	21.697611836105075	27.1101077544954	29.33489466632137	21.857385743078154
35	21.529618411719007	27.352358325920754	29.242171754135093	21.875851508225146
36	21.447873622104872	27.22940335311272	29.283438235393465	22.03928478938894
37	21.26259669192573	26.96108677783658	29.281130014750094	22.495186515487596
38	20.955040365712225	27.353484287210208	29.7094456892572	21.982029657820366
39	20.969677862475088	27.25687680857532	29.5896997061241	22.183745622825487
40	21.009424295992705	27.364349813653405	29.75183813180503	21.87438775854886
41	20.86501976062063	27.120410300293873	29.637496762861293	22.3770731762242
42	20.534268631844437	27.376960580095254	29.316935583754635	22.771835204305678
43	20.47678830801797	26.85794872372288	29.30747750892324	23.357785459335908
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	826.0
1	923.5
2	1021.0
3	4219.0
4	7417.0
5	7417.0
6	8878.0
7	10339.0
8	9263.5
9	8188.0
10	10263.0
11	12338.0
12	12338.0
13	18159.0
14	23980.0
15	37467.0
16	50954.0
17	40371.0
18	29788.0
19	29788.0
20	30297.0
21	30806.0
22	24577.0
23	18348.0
24	20974.0
25	23600.0
26	23600.0
27	27696.0
28	31792.0
29	35356.0
30	38920.0
31	42992.5
32	47065.0
33	47065.0
34	52951.5
35	58838.0
36	66253.5
37	73669.0
38	81395.0
39	89121.0
40	89121.0
41	96824.5
42	104528.0
43	111480.5
44	118433.0
45	125048.5
46	131664.0
47	131664.0
48	136600.5
49	141537.0
50	140960.0
51	140383.0
52	137515.5
53	134648.0
54	134648.0
55	125549.0
56	116450.0
57	105346.5
58	94243.0
59	84988.5
60	75734.0
61	75734.0
62	65676.0
63	55618.0
64	46941.0
65	38264.0
66	31576.0
67	24888.0
68	24888.0
69	20407.0
70	15926.0
71	13464.5
72	11003.0
73	8814.0
74	6625.0
75	6625.0
76	5252.5
77	3880.0
78	3139.0
79	2398.0
80	1885.0
81	1372.0
82	1372.0
83	1100.5
84	829.0
85	668.5
86	508.0
87	353.0
88	198.0
89	198.0
90	137.5
91	77.0
92	52.0
93	27.0
94	19.0
95	11.0
96	11.0
97	7.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1776260.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.13810936440959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.0762519748634	49.721105842391296
2	8.324445194640601	9.845839006373795
3	2.7616731159561274	4.899603802804899
4	1.336964017848575	3.1626209721523795
5	0.7970115111615782	2.356687695588339
6	0.5078333407164851	1.8019382185310986
7	0.36086913630238104	1.493878291422317
8	0.2743138665989938	1.2977922754484288
9	0.2217787945602088	1.1804020746667698
>10	1.2389531447046203	13.164327947664614
>50	0.0668179652630919	2.6716077502011553
>100	0.029750956602127385	3.17985733908792
>500	0.0020975307770839087	0.8541557381932358
>1k	8.580807724434173E-4	1.1733435152024052
>5k	9.53423080492686E-5	0.4055675110452139
>10k+	2.860269241478058E-4	2.7912720192260965
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28799	1.6213279587447782	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10390	0.5849368898697263	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10316	0.5807708330987581	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7193	0.4049519777510049	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4208	0.2369022553004628	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4181	0.2353822075597041	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2780	0.15650861923367074	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2649	0.14913357278776757	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.6298064472543436E-5	0.0
6	0.0	0.0	0.0	5.6298064472543436E-5	1.1259612894508687E-4
7	0.0	0.0	0.0	5.6298064472543436E-5	1.1259612894508687E-4
8	0.0	0.0	0.0	5.6298064472543436E-5	1.688941934176303E-4
9	0.0	0.0	0.0	2.2519225789017374E-4	1.688941934176303E-4
10	0.0	0.0	0.0	0.0014637496762861292	1.688941934176303E-4
11	0.0	0.0	0.0	0.002420816772319368	1.688941934176303E-4
12	0.0	5.6298064472543436E-5	0.0	0.0035467780617702365	1.688941934176303E-4
13	0.0	5.6298064472543436E-5	0.0	0.00377197031966041	1.688941934176303E-4
14	0.0	5.6298064472543436E-5	0.0	0.004109758706495671	2.2519225789017374E-4
15	0.0	5.6298064472543436E-5	0.0	0.005179421931473996	2.8149032236271716E-4
16	0.0	5.6298064472543436E-5	0.0	0.006361681285397408	3.9408645130780404E-4
17	0.0	5.6298064472543436E-5	0.0	0.007769132897210994	3.9408645130780404E-4
18	0.0	5.6298064472543436E-5	0.0	0.009007690315606949	4.503845157803475E-4
19	0.0	5.6298064472543436E-5	0.0	0.011090718701091056	4.503845157803475E-4
20	0.0	5.6298064472543436E-5	0.0	0.019310236114082398	4.503845157803475E-4
21	0.0	5.6298064472543436E-5	0.0	0.028712012880997152	4.503845157803475E-4
22	0.0	5.6298064472543436E-5	0.0	0.049091912220057876	4.503845157803475E-4
23	0.0	5.6298064472543436E-5	0.0	0.06969700381700877	6.192787091979778E-4
24	0.0	5.6298064472543436E-5	0.0	0.09998536250323714	6.755767736705212E-4
25	0.0	5.6298064472543436E-5	0.0	0.12554468377377187	6.755767736705212E-4
26	0.0	5.6298064472543436E-5	0.0	0.14902097665882247	6.755767736705212E-4
27	0.0	5.6298064472543436E-5	0.0	0.19383423597896704	6.755767736705212E-4
28	0.0	5.6298064472543436E-5	0.0	0.37348135971085317	6.755767736705212E-4
29	0.0	5.6298064472543436E-5	0.0	0.680531003344105	6.755767736705212E-4
30	0.0	5.6298064472543436E-5	0.0	1.0402756353236575	6.755767736705212E-4
31	0.0	5.6298064472543436E-5	0.0	1.5294495175255876	7.318748381430646E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	250	0.0	16.279999	5
TAGATCG	80	3.3838657E-4	16.1875	5
TATACCG	80	3.3838657E-4	16.1875	5
AACGCAG	12110	0.0	15.796037	5
CGCACTA	75	0.0041055772	14.8	29
AGCGCGT	100	1.0938973E-4	14.799999	32
TTACACT	315	0.0	14.68254	4
GTATTAG	240	0.0	14.645834	1
CGCAATC	90	8.277147E-4	14.388888	22
TCGGTAT	90	8.277147E-4	14.388888	27
ACGCAGA	13390	0.0	14.341301	6
CGCAGAG	13555	0.0	14.194024	7
ACGCTAT	80	0.0063004065	13.875	11
CCCGCGA	80	0.0063004065	13.875	21
GTGTACG	95	0.0012455413	13.631579	1
AGAGTAC	14125	0.0	13.581947	10
TAGCGAC	110	2.4575423E-4	13.454545	24
ATTGGAC	165	2.5970803E-7	13.454545	3
CAGAGTA	14380	0.0	13.418289	9
GCAGAGT	14415	0.0	13.32154	8
>>END_MODULE