FastQCFastQC Report
Thu 9 Feb 2017
SRR2727416.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727416.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564242
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT329391.2845511461086745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT115520.4505035016195819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113670.44328889394994697No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82610.3221614808586709No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43440.1694067876588871No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40020.15606951293988633No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30800.12011346822959769No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA29890.11656466121372319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG500.007037188418.49999812
AACGCAG123200.017.343755
ACGCAGA137450.015.5725736
CGCAGAG137850.015.5408067
AGACGTA2900.015.31034525
CGAGACG2950.015.05084723
CTATACT3950.014.9873424
TAATACG1001.0941981E-414.7999994
AGAGTAC145300.014.62938710
TAAGACT4100.014.4390244
CAGAGTA148350.014.3909689
TACACCG1551.2118835E-714.3225815
GCAGAGT150250.014.1966728
TATACTG4500.013.97777755
ACTAGAC1907.1377144E-913.6315793
GACCGAT2457.2759576E-1213.59183619
GACGTAG3300.013.45454626
TAATACT4150.013.3734944
TATACAC17900.013.12569937
CGTATAC850.00940886113.0588233
Produced by FastQC (version 0.11.5)