##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727416.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2564242
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.380930504999135	33.0	31.0	34.0	31.0	34.0
2	32.511373731496484	34.0	31.0	34.0	31.0	34.0
3	32.59909439124701	34.0	31.0	34.0	31.0	34.0
4	36.1132178632126	37.0	35.0	37.0	35.0	37.0
5	35.93484117333699	37.0	35.0	37.0	35.0	37.0
6	35.96427287284118	37.0	35.0	37.0	35.0	37.0
7	35.934066285475396	37.0	35.0	37.0	35.0	37.0
8	35.89930279591396	37.0	35.0	37.0	35.0	37.0
9	37.73179598493434	39.0	37.0	39.0	35.0	39.0
10	37.571756097903396	39.0	37.0	39.0	35.0	39.0
11	37.67609414400045	39.0	37.0	39.0	35.0	39.0
12	37.6077000532711	39.0	37.0	39.0	35.0	39.0
13	37.68165680150313	39.0	37.0	39.0	35.0	39.0
14	38.89319104827079	40.0	38.0	41.0	36.0	41.0
15	38.83125890614068	40.0	38.0	41.0	35.0	41.0
16	38.813857272441524	40.0	38.0	41.0	35.0	41.0
17	38.77725698276528	40.0	38.0	41.0	35.0	41.0
18	38.76301027750111	40.0	38.0	41.0	35.0	41.0
19	38.81962193895896	40.0	38.0	41.0	35.0	41.0
20	38.77090071841893	40.0	38.0	41.0	35.0	41.0
21	38.723856406688604	40.0	38.0	41.0	35.0	41.0
22	38.671402699121224	40.0	38.0	41.0	34.0	41.0
23	38.60272041406388	40.0	38.0	41.0	34.0	41.0
24	38.558819331404756	40.0	38.0	41.0	34.0	41.0
25	38.44277451192204	40.0	38.0	41.0	34.0	41.0
26	38.3302784994552	40.0	38.0	41.0	34.0	41.0
27	38.235521842322214	40.0	38.0	41.0	34.0	41.0
28	38.13949892404851	40.0	38.0	41.0	34.0	41.0
29	38.044216575502624	40.0	37.0	41.0	33.0	41.0
30	37.958936013059606	40.0	37.0	41.0	33.0	41.0
31	37.84384352178928	40.0	37.0	41.0	33.0	41.0
32	37.72639165882159	40.0	37.0	41.0	33.0	41.0
33	37.604788471602916	40.0	37.0	41.0	33.0	41.0
34	37.48313341720477	40.0	36.0	41.0	33.0	41.0
35	37.38844578631814	40.0	36.0	41.0	33.0	41.0
36	37.2796795310271	40.0	36.0	41.0	32.0	41.0
37	37.16719989766956	40.0	36.0	41.0	32.0	41.0
38	37.04498990344905	39.0	36.0	41.0	31.0	41.0
39	36.91267477874553	39.0	36.0	41.0	31.0	41.0
40	36.76377892570202	39.0	35.0	41.0	31.0	41.0
41	36.628263245044735	39.0	35.0	41.0	30.0	41.0
42	36.47941184958362	39.0	35.0	41.0	30.0	41.0
43	34.873556006024394	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	12.0
11	17.0
12	19.0
13	7.0
14	9.0
15	11.0
16	16.0
17	35.0
18	54.0
19	145.0
20	267.0
21	610.0
22	1048.0
23	2038.0
24	3525.0
25	5561.0
26	8796.0
27	13306.0
28	19669.0
29	27180.0
30	35912.0
31	46371.0
32	59200.0
33	77083.0
34	104026.0
35	143831.0
36	213769.0
37	361737.0
38	677900.0
39	762084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.710110044215796	18.474699345849572	13.640054253849677	24.175136356084955
2	16.15198565502008	22.211749125082576	38.148583480030354	23.487681739866986
3	18.367689165063204	23.752321348765054	31.659180373771274	26.220809112400467
4	12.75932614784408	15.853807869928033	36.31459121252986	35.072274769698026
5	13.767421327628204	38.30769482755528	34.77078216486588	13.154101679950644
6	32.97609975969507	37.726587428175655	15.420619426715575	13.876693385413702
7	27.88441964525969	31.501706937176756	22.20355957043056	18.410313847132993
8	26.861739258619117	36.86489028726618	19.711751074976544	16.56161937913816
9	26.05257226112044	14.058969473240046	19.49125706544078	40.39720120019874
10	14.722401395812096	28.726695842280098	34.87935226082406	21.67155050108375
11	35.74409123631857	21.882373036554274	22.66950623225109	19.704029494876067
12	20.45310076038065	26.62954588529476	32.18046502631187	20.73688832801272
13	31.241942063190603	19.818488270607844	25.658147709927537	23.281421956274016
14	20.860199622344535	20.442727324488093	29.178798256950788	29.51827479621658
15	23.91154968992786	29.25254324669824	23.81799377749838	23.01791328587551
16	22.679723676626466	27.041402488532672	26.058031964221783	24.220841870619076
17	22.781040167035716	27.403458799910464	27.707213281741737	22.108287751312083
18	22.78552492315468	26.145426211722604	28.410657028470794	22.658391836651923
19	24.00405265961637	26.319902723689886	28.372868083433623	21.30317653326012
20	22.992330677057783	26.674198457087904	28.241250240811905	22.092220625042412
21	22.63230225540335	26.780350684529775	28.41935355555365	22.167993504513227
22	22.766649949575744	26.56387345656143	28.164463416479414	22.505013177383415
23	22.244117364897697	26.377346599891897	28.394784891597595	22.98375114361281
24	22.353584412079673	26.523432655732183	28.55787402281064	22.565108909377507
25	22.677929774178878	26.36408732093149	28.52016307353206	22.43781983135757
26	22.400420865113354	26.712572370314504	28.693196663965416	22.19381010060673
27	22.799486163942404	26.981345754417873	28.238442393502645	21.980725688137078
28	22.43048823005005	27.06760906341913	28.30368584556372	22.1982168609671
29	22.29231874370672	27.1479446947675	28.429142023256777	22.13059453826901
30	22.16502966568678	27.30549612712061	28.378249790776376	22.151224416416234
31	22.307020944201053	26.523822634525136	28.44236230433789	22.72679411693592
32	21.889626642103202	27.044990293427844	28.40133653531921	22.664046529149743
33	22.117452252946485	26.57853665917647	28.508775692777828	22.795235395099215
34	22.201921659500158	26.57529983519496	28.905774104004223	22.317004401300657
35	22.21880774123503	26.745681569836233	28.553974234881107	22.481536454047628
36	21.945666594650586	26.480417994869438	28.8801524973072	22.693762913172783
37	21.88412794112256	26.31713387425992	28.673190751886914	23.12554743273061
38	21.69725010353937	26.43451749093884	29.16861981045471	22.69961259506708
39	21.64487595164575	26.391970804627647	29.17560043084857	22.787552812878037
40	21.679428072701405	26.362566403638972	29.405141948380848	22.552863575278774
41	21.400983214532793	26.207198852526396	29.521082643525848	22.870735289414963
42	21.060102751612366	26.4256649723388	29.241935823529918	23.272296452518912
43	20.861057575689035	25.88800121049417	29.29454396269931	23.956397251117483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	802.0
1	884.5
2	967.0
3	3894.5
4	6822.0
5	6822.0
6	8453.0
7	10084.0
8	8957.5
9	7831.0
10	10210.5
11	12590.0
12	12590.0
13	19324.0
14	26058.0
15	41643.5
16	57229.0
17	44950.5
18	32672.0
19	32672.0
20	34108.5
21	35545.0
22	30467.0
23	25389.0
24	29952.0
25	34515.0
26	34515.0
27	40492.0
28	46469.0
29	52378.0
30	58287.0
31	65035.5
32	71784.0
33	71784.0
34	80002.5
35	88221.0
36	98591.0
37	108961.0
38	120169.0
39	131377.0
40	131377.0
41	144100.5
42	156824.0
43	167111.0
44	177398.0
45	186832.5
46	196267.0
47	196267.0
48	203940.5
49	211614.0
50	212376.5
51	213139.0
52	204772.0
53	196405.0
54	196405.0
55	182689.0
56	168973.0
57	155513.0
58	142053.0
59	128044.0
60	114035.0
61	114035.0
62	98362.0
63	82689.0
64	68671.5
65	54654.0
66	45301.0
67	35948.0
68	35948.0
69	29068.0
70	22188.0
71	18494.0
72	14800.0
73	11884.5
74	8969.0
75	8969.0
76	7077.0
77	5185.0
78	4215.0
79	3245.0
80	2561.5
81	1878.0
82	1878.0
83	1550.5
84	1223.0
85	951.5
86	680.0
87	493.5
88	307.0
89	307.0
90	202.5
91	98.0
92	66.5
93	35.0
94	27.0
95	19.0
96	19.0
97	13.0
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2564242.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.61649975669119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.660402394248	53.85798468315055
2	8.714824957060257	11.088133195208604
3	2.500175337735786	4.771572112942619
4	1.2232265351376574	3.11269562299852
5	0.7122490932134091	2.265539713255718
6	0.4381960555872348	1.672589955818902
7	0.30547474324336854	1.360326375045194
8	0.2269999557470068	1.1552754103638696
9	0.18184715472030463	1.041163152661732
>10	0.9600394587701251	10.896256741032884
>50	0.052156713756670414	2.259618348115238
>100	0.022204569915684057	2.6335609192503355
>500	0.001468687243053927	0.6310213788524222
>1k	4.895624143465472E-4	0.7580899074773861
>5k	6.11953017933184E-5	0.32160328278491696
>10k+	1.8358590537995522E-4	2.174569201041023
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32939	1.2845511461086745	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11552	0.4505035016195819	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11367	0.44328889394994697	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8261	0.3221614808586709	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4344	0.1694067876588871	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4002	0.15606951293988633	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3080	0.12011346822959769	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2989	0.11656466121372319	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.899787929532392E-5	0.0
4	0.0	0.0	0.0	7.799575859064785E-5	0.0
5	0.0	0.0	0.0	7.799575859064785E-5	0.0
6	0.0	0.0	0.0	7.799575859064785E-5	7.799575859064785E-5
7	0.0	0.0	0.0	7.799575859064785E-5	7.799575859064785E-5
8	0.0	0.0	0.0	7.799575859064785E-5	7.799575859064785E-5
9	0.0	0.0	0.0	1.559915171812957E-4	7.799575859064785E-5
10	3.899787929532392E-5	0.0	0.0	3.509809136579153E-4	7.799575859064785E-5
11	3.899787929532392E-5	0.0	0.0	7.019618273158306E-4	7.799575859064785E-5
12	3.899787929532392E-5	0.0	0.0	0.0011699363788597176	7.799575859064785E-5
13	3.899787929532392E-5	0.0	0.0	0.0012869300167456894	1.1699363788597176E-4
14	7.799575859064785E-5	0.0	0.0	0.0014039236546316612	2.339872757719435E-4
15	1.1699363788597176E-4	0.0	0.0	0.0017549045682895765	2.339872757719435E-4
16	1.1699363788597176E-4	0.0	0.0	0.002495864274900731	2.339872757719435E-4
17	1.1699363788597176E-4	0.0	0.0	0.003119830343625914	2.339872757719435E-4
18	1.1699363788597176E-4	0.0	0.0	0.003392815498693181	2.7298515506726746E-4
19	1.1699363788597176E-4	0.0	0.0	0.003899787929532392	2.7298515506726746E-4
20	1.1699363788597176E-4	0.0	0.0	0.00686362675597701	2.7298515506726746E-4
21	1.1699363788597176E-4	0.0	0.0	0.0099444592203076	2.7298515506726746E-4
22	1.1699363788597176E-4	0.0	0.0	0.0171200690106472	2.7298515506726746E-4
23	1.1699363788597176E-4	0.0	0.0	0.02199480392256269	3.509809136579153E-4
24	1.1699363788597176E-4	0.0	0.0	0.03073032888471525	3.509809136579153E-4
25	1.1699363788597176E-4	0.0	0.0	0.03646301714112787	3.509809136579153E-4
26	1.1699363788597176E-4	0.0	0.0	0.04215670751824516	3.509809136579153E-4
27	1.1699363788597176E-4	0.0	0.0	0.06251360051040425	3.509809136579153E-4
28	1.1699363788597176E-4	0.0	0.0	0.2075467136097139	3.8997879295323923E-4
29	1.1699363788597176E-4	0.0	0.0	0.4458627539834384	3.8997879295323923E-4
30	1.1699363788597176E-4	0.0	0.0	0.7424806239036721	3.8997879295323923E-4
31	1.1699363788597176E-4	3.899787929532392E-5	0.0	1.2418484682802948	3.8997879295323923E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGG	50	0.0070371884	18.499998	12
AACGCAG	12320	0.0	17.34375	5
ACGCAGA	13745	0.0	15.572573	6
CGCAGAG	13785	0.0	15.540806	7
AGACGTA	290	0.0	15.310345	25
CGAGACG	295	0.0	15.050847	23
CTATACT	395	0.0	14.987342	4
TAATACG	100	1.0941981E-4	14.799999	4
AGAGTAC	14530	0.0	14.629387	10
TAAGACT	410	0.0	14.439024	4
CAGAGTA	14835	0.0	14.390968	9
TACACCG	155	1.2118835E-7	14.322581	5
GCAGAGT	15025	0.0	14.196672	8
TATACTG	450	0.0	13.9777775	5
ACTAGAC	190	7.1377144E-9	13.631579	3
GACCGAT	245	7.2759576E-12	13.591836	19
GACGTAG	330	0.0	13.454546	26
TAATACT	415	0.0	13.373494	4
TATACAC	1790	0.0	13.125699	37
CGTATAC	85	0.009408861	13.058823	3
>>END_MODULE