FastQCFastQC Report
Thu 9 Feb 2017
SRR2727414.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727414.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363596
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT236631.7353380326724337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT84220.6176316152291441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT83760.6142581820421884No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63050.46238035312511916No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43390.31820275213479654No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA42080.30859580110237933No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41470.3041223353544598No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT37180.272661404110895No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG33660.24684730668027774No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.15583794613653898No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG20460.15004444131546293No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA19450.14263755540497333No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT17390.12753044156773707No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA16260.11924352960847641No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG14460.10604313887691075No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG14160.10384307375498314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGA701.9234722E-723.78571318
TATACCG551.9029956E-523.5454545
ACGACCG753.741825E-722.217
TCTATAC1450.020.4137943
TCGGACG851.2456567E-619.58823413
CTAAGCG701.2194089E-418.59
CCGCTTA609.238021E-418.525
TACTATA1750.017.971432
ACCGATT953.6073852E-617.52631620
TAATACT1705.456968E-1217.4117644
CAGTGCG852.7237193E-517.4117649
TACTTAC3500.016.91428631
AGTCGGT1757.2759576E-1216.91428611
GTACGAA1107.810213E-716.8181821
TCCAACG904.4469336E-516.4444452
GTATAAG1801.0913936E-1116.4444451
GATCTCG3200.016.187519
TCGTTAC2650.016.05660423
CTCGTTA2900.015.94827622
ATAATAC1751.3096724E-1015.8571433
Produced by FastQC (version 0.11.5)