##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727414.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1363596 Sequences flagged as poor quality 0 Sequence length 43 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37657634665986 33.0 31.0 34.0 31.0 34.0 2 32.46293183611568 34.0 31.0 34.0 31.0 34.0 3 32.566351030657174 34.0 31.0 34.0 31.0 34.0 4 36.07252294667922 37.0 35.0 37.0 35.0 37.0 5 35.90218290461398 37.0 35.0 37.0 35.0 37.0 6 35.94491183605702 37.0 35.0 37.0 35.0 37.0 7 35.90556220464126 37.0 35.0 37.0 35.0 37.0 8 35.862839140038545 37.0 35.0 37.0 35.0 37.0 9 37.69721457088463 39.0 37.0 39.0 35.0 39.0 10 37.54427557722375 39.0 37.0 39.0 35.0 39.0 11 37.66038768080868 39.0 37.0 39.0 35.0 39.0 12 37.610375067101984 39.0 37.0 39.0 35.0 39.0 13 37.69067891076242 39.0 37.0 39.0 35.0 39.0 14 38.862484929553915 40.0 38.0 41.0 36.0 41.0 15 38.80832445973734 40.0 38.0 41.0 35.0 41.0 16 38.76853554865224 40.0 38.0 41.0 35.0 41.0 17 38.7378600406572 40.0 38.0 41.0 35.0 41.0 18 38.74987166286789 40.0 38.0 41.0 35.0 41.0 19 38.81506692598101 40.0 38.0 41.0 35.0 41.0 20 38.77185618027627 40.0 38.0 41.0 35.0 41.0 21 38.71424894176867 40.0 38.0 41.0 35.0 41.0 22 38.64062669588353 40.0 38.0 41.0 35.0 41.0 23 38.555065429936725 40.0 38.0 41.0 34.0 41.0 24 38.5105683794907 40.0 38.0 41.0 34.0 41.0 25 38.39275122543627 40.0 38.0 41.0 34.0 41.0 26 38.268573683114354 40.0 38.0 41.0 34.0 41.0 27 38.155228528097766 40.0 37.0 41.0 34.0 41.0 28 38.01577740034438 40.0 37.0 41.0 33.0 41.0 29 37.85173907814338 40.0 37.0 41.0 33.0 41.0 30 37.701718837544256 40.0 37.0 41.0 33.0 41.0 31 37.52280880847407 39.0 36.0 41.0 33.0 41.0 32 37.347932232127405 39.0 36.0 41.0 33.0 41.0 33 37.171723883026935 39.0 35.0 41.0 32.0 41.0 34 36.974158035077835 39.0 35.0 41.0 31.0 41.0 35 36.79717306298933 39.0 35.0 41.0 31.0 41.0 36 36.6011457939155 39.0 35.0 41.0 31.0 41.0 37 36.437766757896036 39.0 35.0 41.0 30.0 41.0 38 36.27059114283116 39.0 35.0 41.0 30.0 41.0 39 36.097480485422366 39.0 35.0 41.0 30.0 41.0 40 35.88090020797949 39.0 35.0 40.0 28.0 41.0 41 35.68548602371963 39.0 35.0 40.0 27.0 41.0 42 35.456353641401115 39.0 35.0 40.0 25.0 41.0 43 33.88729139715869 37.0 33.0 40.0 21.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 6.0 10 6.0 11 4.0 12 4.0 13 2.0 14 2.0 15 5.0 16 9.0 17 14.0 18 35.0 19 79.0 20 165.0 21 330.0 22 672.0 23 1196.0 24 2275.0 25 3601.0 26 5944.0 27 9007.0 28 13290.0 29 18763.0 30 23809.0 31 28736.0 32 34537.0 33 44533.0 34 59814.0 35 81667.0 36 121321.0 37 209469.0 38 342894.0 39 361407.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.4915590834822 17.01031683871176 12.392013470265386 23.10611060754065 2 15.683824241197538 24.289378965617384 36.67310552392351 23.35369126926157 3 19.199748312550053 26.640661896925483 30.210414228261158 23.94917556226331 4 12.854320487886442 15.060912469675769 34.845218085121985 37.239548957315805 5 14.778130766004008 38.05386639444527 33.699130827605835 13.468872011944889 6 34.251640515225915 35.284717760979056 15.386815449737313 15.076826274057712 7 28.238349188469314 30.350558376527946 23.470001378707476 17.941091056295267 8 28.242822654217232 35.3747004244659 20.958480371018982 15.423996550297888 9 26.23320983634449 14.330050836171418 20.42518458546373 39.011554742020365 10 14.5169830360312 28.872187950096656 34.76146893948061 21.849360074391537 11 34.129683571967064 22.244198428273478 22.71369232529283 20.912425674466633 12 19.839600585510663 26.981231977799876 32.61295867691017 20.56620875977929 13 31.520333001856855 19.13785314711982 26.301778532644565 23.040035318378756 14 20.817896209727806 20.250499414782677 30.48630239455088 28.445301980938638 15 24.61806869483337 28.845273820105078 24.48929154969654 22.047365935365022 16 22.118648045315474 26.191848612052247 27.285574319666527 24.403929022965745 17 22.07963355715329 27.53542838201344 29.152696253142423 21.23224180769084 18 21.58014543897166 26.319232382611858 30.190833648676 21.90978852974048 19 22.547440737579166 27.688772921011797 29.65159768729154 20.112188654117496 20 21.368865851762546 27.918019706716652 29.292473723888897 21.42064071763191 21 20.508713724592916 29.176383620955182 29.550761369203194 20.764141285248712 22 21.097891164245127 28.395727180191198 28.55662527610817 21.949756379455497 23 20.56232197806388 27.718473800157817 29.14272262458969 22.57648159718861 24 20.722853396460536 27.910026136773645 29.34131516959569 22.025805297170127 25 21.21640133881296 27.887365466017794 28.82041308422729 22.07582011094195 26 20.989207947221907 28.330971930102468 29.79064180299737 20.889178319678262 27 21.875907526862797 28.584566103156657 29.408637162326674 20.13088920765388 28 21.41440719978645 28.993778215835185 29.068947107501046 20.522867476877316 29 21.04985640908304 28.798265761999886 29.657537863120748 20.49433996579632 30 20.74382735062291 28.883261611210358 29.87321758057372 20.49969345759301 31 20.587109378437603 28.206301573193233 29.82635619347666 21.380232854892505 32 20.161323441840544 28.65166808937544 29.374902830457117 21.812105638326894 33 21.093564369505337 28.186207645079627 29.077380690468434 21.642847294946595 34 21.4887694009076 28.649101346733197 29.12064863786635 20.741480614492854 35 21.194547358601813 28.76533812067504 29.156949712378154 20.883164808344993 36 20.82757649626429 28.857447513779743 29.252579209677943 21.06239678027803 37 19.987884974728583 28.642207809351156 29.36888931912385 22.001017896796412 38 20.71207307736309 28.36213951932977 29.506466724748385 21.41932067855875 39 20.08828127979255 28.27677699259898 29.666191452600334 21.96875027500814 40 20.688239038542207 28.361992848321645 29.97632729928806 20.97344081384809 41 20.78108178668755 27.829943766335486 29.657904540641074 21.731069906335897 42 19.889248721762165 28.27934373524123 29.83706317707004 21.994344365926565 43 19.935890102347027 27.579796361972313 29.497446457748485 22.986867077932175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 202.0 1 413.0 2 624.0 3 4092.5 4 7561.0 5 7561.0 6 9256.5 7 10952.0 8 9794.5 9 8637.0 10 10269.5 11 11902.0 12 11902.0 13 17837.0 14 23772.0 15 35609.0 16 47446.0 17 39680.5 18 31915.0 19 31915.0 20 31480.0 21 31045.0 22 23717.5 23 16390.0 24 17058.0 25 17726.0 26 17726.0 27 20197.5 28 22669.0 29 25232.0 30 27795.0 31 30141.5 32 32488.0 33 32488.0 34 35699.5 35 38911.0 36 43769.0 37 48627.0 38 54042.0 39 59457.0 40 59457.0 41 64647.0 42 69837.0 43 75355.5 44 80874.0 45 88018.0 46 95162.0 47 95162.0 48 102500.5 49 109839.0 50 111613.0 51 113387.0 52 116300.5 53 119214.0 54 119214.0 55 110011.0 56 100808.0 57 84980.5 58 69153.0 59 62235.0 60 55317.0 61 55317.0 62 47778.5 63 40240.0 64 33674.5 65 27109.0 66 22312.0 67 17515.0 68 17515.0 69 14067.5 70 10620.0 71 8719.5 72 6819.0 73 5429.0 74 4039.0 75 4039.0 76 3172.0 77 2305.0 78 1868.5 79 1432.0 80 1125.0 81 818.0 82 818.0 83 672.5 84 527.0 85 403.0 86 279.0 87 195.5 88 112.0 89 112.0 90 78.5 91 45.0 92 30.0 93 15.0 94 11.0 95 7.0 96 7.0 97 5.0 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 1363596.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.191040318564134 #Duplication Level Percentage of deduplicated Percentage of total 1 85.00477268436566 50.315209272306674 2 8.177961988190443 9.681241555333308 3 2.502042161182855 4.442954353239652 4 1.2324750397458784 2.9180591907688886 5 0.739177888720934 2.1876354106935962 6 0.4851261746987287 1.7229073779709942 7 0.3323655629841088 1.3771144407366256 8 0.2384193742830583 1.1289832636732269 9 0.19129333941337429 1.0190566589300831 >10 0.9949127924638544 10.406002368958914 >50 0.05721141480656395 2.33451980942889 >100 0.03781545246064637 4.612132782391797 >500 0.0035838014198097735 1.5000441573727357 >1k 0.0023480078267719205 2.9333042743845557 >5k 3.707380779113559E-4 1.6899361275555815 >10k+ 1.2357935930378528E-4 1.7308989562545007 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 23663 1.7353380326724337 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 8422 0.6176316152291441 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 8376 0.6142581820421884 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6305 0.46238035312511916 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4339 0.31820275213479654 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 4208 0.30859580110237933 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4147 0.3041223353544598 No Hit GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT 3718 0.272661404110895 No Hit GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG 3366 0.24684730668027774 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2125 0.15583794613653898 No Hit GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG 2046 0.15004444131546293 No Hit GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1945 0.14263755540497333 No Hit CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT 1739 0.12753044156773707 No Hit GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 1626 0.11924352960847641 No Hit TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG 1446 0.10604313887691075 No Hit TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG 1416 0.10384307375498314 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.333550406425364E-5 0.0 3 0.0 0.0 0.0 7.333550406425364E-5 0.0 4 7.333550406425364E-5 0.0 0.0 1.4667100812850727E-4 0.0 5 7.333550406425364E-5 0.0 0.0 1.4667100812850727E-4 0.0 6 7.333550406425364E-5 0.0 0.0 1.4667100812850727E-4 1.4667100812850727E-4 7 7.333550406425364E-5 0.0 0.0 1.4667100812850727E-4 1.4667100812850727E-4 8 7.333550406425364E-5 0.0 0.0 1.4667100812850727E-4 2.200065121927609E-4 9 7.333550406425364E-5 0.0 0.0 2.200065121927609E-4 2.9334201625701454E-4 10 7.333550406425364E-5 0.0 0.0 2.9334201625701454E-4 2.9334201625701454E-4 11 7.333550406425364E-5 0.0 0.0 7.333550406425363E-4 2.9334201625701454E-4 12 7.333550406425364E-5 0.0 0.0 8.800260487710436E-4 2.9334201625701454E-4 13 7.333550406425364E-5 0.0 0.0 9.533615528352973E-4 2.9334201625701454E-4 14 7.333550406425364E-5 0.0 0.0 0.0012467035690923117 4.400130243855218E-4 15 7.333550406425364E-5 0.0 0.0 0.0020533941137991017 4.400130243855218E-4 16 7.333550406425364E-5 0.0 0.0 0.0030800911706986528 4.400130243855218E-4 17 7.333550406425364E-5 0.0 0.0 0.004473465747919471 4.400130243855218E-4 18 7.333550406425364E-5 0.0 0.0 0.005280156292626262 4.400130243855218E-4 19 7.333550406425364E-5 0.0 0.0 0.0072602149023611095 4.400130243855218E-4 20 7.333550406425364E-5 0.0 0.0 0.010046964056802749 4.400130243855218E-4 21 7.333550406425364E-5 0.0 0.0 0.015253784845364757 5.133485284497754E-4 22 7.333550406425364E-5 0.0 0.0 0.02442072285339646 5.133485284497754E-4 23 7.333550406425364E-5 0.0 0.0 0.03754777808089786 5.866840325140291E-4 24 1.4667100812850727E-4 0.0 0.0 0.06182182992616581 5.866840325140291E-4 25 1.4667100812850727E-4 0.0 0.0 0.08910263743806816 5.866840325140291E-4 26 1.4667100812850727E-4 0.0 0.0 0.11286334075488634 5.866840325140291E-4 27 1.4667100812850727E-4 0.0 0.0 0.1372840636082828 5.866840325140291E-4 28 1.4667100812850727E-4 0.0 0.0 0.22690004957480076 5.866840325140291E-4 29 1.4667100812850727E-4 0.0 0.0 0.3997518326542466 5.866840325140291E-4 30 1.4667100812850727E-4 0.0 0.0 0.6221050809770636 5.866840325140291E-4 31 1.4667100812850727E-4 0.0 0.0 1.0587446721756297 5.866840325140291E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACCGA 70 1.9234722E-7 23.785713 18 TATACCG 55 1.9029956E-5 23.545454 5 ACGACCG 75 3.741825E-7 22.2 17 TCTATAC 145 0.0 20.413794 3 TCGGACG 85 1.2456567E-6 19.588234 13 CTAAGCG 70 1.2194089E-4 18.5 9 CCGCTTA 60 9.238021E-4 18.5 25 TACTATA 175 0.0 17.97143 2 ACCGATT 95 3.6073852E-6 17.526316 20 TAATACT 170 5.456968E-12 17.411764 4 CAGTGCG 85 2.7237193E-5 17.411764 9 TACTTAC 350 0.0 16.914286 31 AGTCGGT 175 7.2759576E-12 16.914286 11 GTACGAA 110 7.810213E-7 16.818182 1 TCCAACG 90 4.4469336E-5 16.444445 2 GTATAAG 180 1.0913936E-11 16.444445 1 GATCTCG 320 0.0 16.1875 19 TCGTTAC 265 0.0 16.056604 23 CTCGTTA 290 0.0 15.948276 22 ATAATAC 175 1.3096724E-10 15.857143 3 >>END_MODULE