FastQCFastQC Report
Thu 9 Feb 2017
SRR2727412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628447
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT445451.6947269623469676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT162810.6194151907951729No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT157230.5981859249967757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112590.4283517986095972No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62600.23816344784581925No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62090.23622313860618077No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA46290.176111597456597No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42070.16005648963056893No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT31750.1207937614872965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG851.2465571E-619.5882364
TAGATCG1052.262641E-819.380955
ACGGATG5650.015.71681428
AACGCAG191800.015.5581325
CGAACGT1201.9377094E-615.4166674
TAATACC2900.015.3103454
TGCGACG1001.0942142E-414.822
TATCGAC750.004106273414.814
ATCGATA1803.3360266E-914.38888923
TACGGAT5850.014.2307727
CGAACGG1304.448915E-614.23076929
ACGCAGA211850.014.2079316
GGCGTCA4700.014.17021212
CGCAGAG213000.014.131227
GCGTTAT1051.6569105E-414.0952381
CCGCTAG1051.6569105E-414.09523836
TGTTACG2900.014.03448324
CGTCAGA5150.014.00970814
TATACAG5950.013.9915975
TATACTG2800.013.8755
Produced by FastQC (version 0.11.5)