FastQCFastQC Report
Thu 9 Feb 2017
SRR2727411.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727411.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797552
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT543393.0229445379048836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT176430.9815015087185238No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT174730.9720442023374011No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137560.765262979874852No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA78650.43753949816194465No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71370.39703997436513655No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67670.3764564251826929No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA44340.2466688029052845No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43550.24227393699876276No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT37600.20917336466483305No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA34290.19075943282864694No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG32630.1815246513035506No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25550.1421377517868746No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22910.127451111289131No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG22100.12294498295459602No Hit
GTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA18850.10486483840244955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC603.7277918E-521.5833343
GTACTAA1101.7571438E-920.1818181
CGCAATC555.1446457E-420.18181822
AGATCGA1002.8771683E-718.56
TAGATCG609.2395785E-418.55
CGCTTAT1950.018.02564226
CCGCTTA2050.017.14634125
AGTCGGT5400.017.1296311
CCCTATA650.001580402417.0769232
ACGCGCA700.00259325715.85714219
CCGATCG957.063217E-515.5789489
ATACTTC2750.015.4727286
GCGTTAT855.3662405E-415.2352941
CTACACG855.3662405E-415.2352944
CGGTGAT5850.014.86324714
GGCGTCA2500.014.79999912
TATACTG2500.014.7999995
GGCAGTC7150.014.7482518
GTCCTAG1405.999773E-714.5357141
GTTCTAT2450.014.3469381
Produced by FastQC (version 0.11.5)