FastQCFastQC Report
Thu 9 Feb 2017
SRR2727410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4815873
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT450410.9352613742098266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT179930.3736186564720457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT173300.3598516821353055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121290.25185464816036474No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76790.1594518792335263No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76700.15926499722895515No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA49770.103345748527837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCT1005.885626E-616.6500024
CTTATAC66800.015.95209637
TACTTAC17550.014.75783431
TCGTTAC8150.014.75460123
GGAGTGT19550.014.6675196
GTACTAA3200.014.4531251
TTAGTAC3250.014.230773
AGTGTAC22250.014.217978527
TAGATCG1702.442357E-814.1470595
AACGCAG212900.013.79027755
CATTACG7450.013.40939524
TATACAC28900.013.37889237
TAAGACT8200.013.3109754
CTCGTTA10150.013.30541922
TAGACAG8700.013.18390755
ATTACGG7300.013.17808225
GAGTGTC18850.013.1511947
ACAGCTA24350.013.14373819
CTACAGT24750.012.93131323
TAATACG1451.3740157E-512.758624
Produced by FastQC (version 0.11.5)