##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727410.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4815873
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26628318479329	33.0	31.0	34.0	30.0	34.0
2	32.399793142385604	34.0	31.0	34.0	31.0	34.0
3	32.49492833386595	34.0	31.0	34.0	30.0	34.0
4	36.039365863676224	37.0	35.0	37.0	35.0	37.0
5	35.84355401398666	37.0	35.0	37.0	35.0	37.0
6	35.86030092570963	37.0	35.0	37.0	35.0	37.0
7	35.82871537517705	37.0	35.0	37.0	35.0	37.0
8	35.78203827218866	37.0	35.0	37.0	35.0	37.0
9	37.613583871501596	39.0	37.0	39.0	35.0	39.0
10	37.44111462241633	39.0	37.0	39.0	35.0	39.0
11	37.552375862071116	39.0	37.0	39.0	35.0	39.0
12	37.48067172867723	39.0	37.0	39.0	35.0	39.0
13	37.56095810666103	39.0	37.0	39.0	35.0	39.0
14	38.758920552929865	40.0	38.0	41.0	35.0	41.0
15	38.68395782031627	40.0	38.0	41.0	35.0	41.0
16	38.652662559830794	40.0	38.0	41.0	35.0	41.0
17	38.59855959656743	40.0	38.0	41.0	34.0	41.0
18	38.57204477775888	40.0	38.0	41.0	34.0	41.0
19	38.627128040959555	40.0	38.0	41.0	34.0	41.0
20	38.58460677845948	40.0	38.0	41.0	34.0	41.0
21	38.534188297739576	40.0	38.0	41.0	34.0	41.0
22	38.49029199897921	40.0	38.0	41.0	34.0	41.0
23	38.411964144403306	40.0	38.0	41.0	34.0	41.0
24	38.36778170852927	40.0	38.0	41.0	34.0	41.0
25	38.25329239371553	40.0	38.0	41.0	34.0	41.0
26	38.133277393319965	40.0	38.0	41.0	34.0	41.0
27	38.03593138772555	40.0	37.0	41.0	33.0	41.0
28	37.934377422328204	40.0	37.0	41.0	33.0	41.0
29	37.83200200669744	40.0	37.0	41.0	33.0	41.0
30	37.75157505191686	40.0	37.0	41.0	33.0	41.0
31	37.628482104906006	40.0	37.0	41.0	33.0	41.0
32	37.512934207359706	40.0	36.0	41.0	33.0	41.0
33	37.394746082382156	40.0	36.0	41.0	32.0	41.0
34	37.27057191915152	39.0	36.0	41.0	32.0	41.0
35	37.17689482260018	39.0	36.0	41.0	31.0	41.0
36	37.05463557697639	39.0	36.0	41.0	31.0	41.0
37	36.95388769595876	39.0	36.0	41.0	31.0	41.0
38	36.84146591905559	39.0	35.0	41.0	31.0	41.0
39	36.70942693048592	39.0	35.0	41.0	30.0	41.0
40	36.574155298530506	39.0	35.0	41.0	30.0	41.0
41	36.44735710430902	39.0	35.0	40.0	30.0	41.0
42	36.30648233456323	39.0	35.0	40.0	30.0	41.0
43	34.6554566534458	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	13.0
11	32.0
12	22.0
13	14.0
14	14.0
15	22.0
16	32.0
17	68.0
18	139.0
19	328.0
20	631.0
21	1305.0
22	2477.0
23	4511.0
24	7648.0
25	12206.0
26	18885.0
27	28065.0
28	41450.0
29	57299.0
30	75188.0
31	96277.0
32	121632.0
33	157327.0
34	211340.0
35	287048.0
36	423870.0
37	704103.0
38	1291517.0
39	1272403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.566000806084375	17.47759959616875	12.498336231042638	25.45806336670423
2	16.63839557230849	21.68352446171234	36.76998957406062	24.90809039191856
3	18.539878439485427	23.493622028653995	30.744000101331576	27.222499430529002
4	13.468544540107267	15.122284163224403	35.093118111711	36.316053184957326
5	14.48267012024611	37.294255890884166	34.37443221613194	13.848641772737777
6	34.73646418832058	35.82233169354757	15.16406267357964	14.277141444552214
7	29.253491526873738	30.98520247523139	21.756138502821816	18.005167495073064
8	27.53743713756571	35.950013632004	19.84730494346508	16.66524428696521
9	27.111055461803087	13.622888311215847	19.050959192653128	40.21509703432794
10	14.841255157683767	27.80690022349011	34.64584718077076	22.705997438055363
11	36.375336309740725	21.19229473036353	22.282398227694127	20.149970732201616
12	20.87015168381724	25.316780571248454	31.690453631148497	22.122614113785808
13	31.324725548202785	19.174010610329635	25.250354400957004	24.25090944051058
14	21.447450960604648	19.573460512766843	28.0843784709439	30.89471005568461
15	25.027528757506683	28.123042281222947	23.038294406850017	23.811134554420352
16	23.65483890459736	26.31009995487838	25.36260819170273	24.672452948821533
17	23.557784850223417	26.554624675526117	26.675122039140152	23.21246843511031
18	23.38651787536756	25.967981298510157	27.457057110932947	23.188443715189333
19	24.62760126772446	26.070953283028853	27.23134517874537	22.07010027050132
20	23.577054461361417	25.914865279877603	27.193304308481558	23.31477595027942
21	23.117034024775982	26.553129619489553	27.63496877928467	22.694867576449795
22	23.724068304957378	25.710914718888972	26.788621709916356	23.776395266237298
23	22.899129607446046	25.759254864071373	27.471675436623848	23.869940091858734
24	22.931979310916216	25.698975035263594	27.583970756703923	23.785074897116264
25	23.396090386935036	25.5513382516524	27.267351111626077	23.785220249786487
26	23.051417676504343	25.921323091368897	27.71784056597838	23.309418666148378
27	23.730006999769305	26.38630628340905	27.13657523776063	22.747111479061015
28	23.287781882952476	26.66534188090093	26.879363305469226	23.16751293067737
29	23.088731783417046	26.679295737242242	27.380290136388563	22.851682342952152
30	23.560588080291982	26.36286297416896	27.006629950582166	23.069918994956883
31	23.16597634530645	25.733880440784052	27.535879787527616	23.564263426381885
32	22.589756831212117	26.392535683561423	27.428090400224427	23.589617085002036
33	23.065184650841083	25.55179507433024	27.489491521059627	23.893528753769047
34	23.279974368094837	25.852861983694336	27.962780579969614	22.90438306824121
35	23.200445692816235	25.86060720455045	27.45352711751327	23.48541998512004
36	22.888684979857235	25.700656973304735	27.89510852964769	23.51554951719034
37	22.450405149803576	25.47342922041341	28.00173509558911	24.074430534193905
38	22.756351755953695	25.316344513237787	28.168226196994812	23.759077533813702
39	22.101621035272316	25.396620716534674	28.73350273148814	23.768255516704865
40	22.73637614613176	25.154566991280706	28.790252566876244	23.31880429571129
41	22.18488735064234	25.125849456578276	28.900596008241912	23.788667184537466
42	21.7749305266148	25.48345855465873	28.81317675943697	23.9284341592895
43	21.278821098479963	24.961600939227427	28.625069639502538	25.134508322790072
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	996.0
1	1465.5
2	1935.0
3	8026.5
4	14118.0
5	14118.0
6	16955.0
7	19792.0
8	17634.0
9	15476.0
10	18881.5
11	22287.0
12	22287.0
13	32314.0
14	42341.0
15	63604.5
16	84868.0
17	70778.0
18	56688.0
19	56688.0
20	57331.0
21	57974.0
22	47906.0
23	37838.0
24	42434.0
25	47030.0
26	47030.0
27	55784.5
28	64539.0
29	73848.0
30	83157.0
31	94618.5
32	106080.0
33	106080.0
34	121677.0
35	137274.0
36	156051.0
37	174828.0
38	197930.0
39	221032.0
40	221032.0
41	242091.0
42	263150.0
43	286005.5
44	308861.0
45	337233.0
46	365605.0
47	365605.0
48	387368.5
49	409132.0
50	416424.5
51	423717.0
52	418287.0
53	412857.0
54	412857.0
55	386417.5
56	359978.0
57	329398.0
58	298818.0
59	273607.5
60	248397.0
61	248397.0
62	216311.5
63	184226.0
64	155821.5
65	127417.0
66	105616.0
67	83815.0
68	83815.0
69	68763.0
70	53711.0
71	44297.5
72	34884.0
73	28417.0
74	21950.0
75	21950.0
76	17583.5
77	13217.0
78	10615.0
79	8013.0
80	6324.5
81	4636.0
82	4636.0
83	3741.0
84	2846.0
85	2150.5
86	1455.0
87	1037.0
88	619.0
89	619.0
90	400.5
91	182.0
92	133.0
93	84.0
94	62.0
95	40.0
96	40.0
97	24.5
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4815873.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.812474045993184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.74935915730431	42.64573434725716
2	9.751995680498794	10.300540375459605
3	3.3481336708331795	5.304696677801796
4	1.663257108072955	3.5136289140766648
5	0.9989241592984425	2.637782811843228
6	0.6405271927900525	2.0296695446986512
7	0.4440493992093961	1.641594315961957
8	0.35028685108030744	1.4799612185065134
9	0.26045240158279737	1.2379622128927272
>10	1.6202930242980873	15.892898239807998
>50	0.1177411001248259	4.251752826139688
>100	0.050743987093480165	4.8101764023644655
>500	0.0029418533518325257	1.0519142566716784
>1k	0.0010590663776186774	0.9676842861226177
>5k	7.844936130487883E-5	0.3179625823148276
>10k+	1.5689872260975766E-4	1.9160409880803537
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	45041	0.9352613742098266	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17993	0.3736186564720457	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17330	0.3598516821353055	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12129	0.25185464816036474	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7679	0.1594518792335263	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7670	0.15926499722895515	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	4977	0.103345748527837	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.0764667174570425E-5	0.0
6	0.0	0.0	0.0	2.0764667174570425E-5	0.0
7	0.0	0.0	0.0	2.0764667174570425E-5	0.0
8	0.0	0.0	0.0	2.0764667174570425E-5	2.0764667174570425E-5
9	0.0	0.0	0.0	4.152933434914085E-5	2.0764667174570425E-5
10	2.0764667174570425E-5	2.0764667174570425E-5	0.0	1.661173373965634E-4	2.0764667174570425E-5
11	2.0764667174570425E-5	2.0764667174570425E-5	0.0	4.360580106659789E-4	2.0764667174570425E-5
12	2.0764667174570425E-5	2.0764667174570425E-5	0.0	7.059986839353944E-4	2.0764667174570425E-5
13	2.0764667174570425E-5	2.0764667174570425E-5	0.0	8.098220198082466E-4	2.0764667174570425E-5
14	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.0010382333587285212	4.152933434914085E-5
15	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.0014950560365690707	8.30586686982817E-5
16	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.002201054720504465	8.30586686982817E-5
17	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.0033015820807566977	8.30586686982817E-5
18	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.003986816097517522	1.0382333587285213E-4
19	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.005087343457769755	1.0382333587285213E-4
20	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.007869808859162192	1.0382333587285213E-4
21	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.011981212959727136	1.0382333587285213E-4
22	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.01964337514714362	1.0382333587285213E-4
23	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.028987475375700313	1.2458800304742255E-4
24	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.04530850377491267	1.2458800304742255E-4
25	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.06086123948866592	1.2458800304742255E-4
26	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.07516809517194493	1.2458800304742255E-4
27	2.0764667174570425E-5	2.0764667174570425E-5	0.0	0.10035563645469887	1.2458800304742255E-4
28	4.152933434914085E-5	2.0764667174570425E-5	0.0	0.20806196508919567	1.2458800304742255E-4
29	4.152933434914085E-5	2.0764667174570425E-5	0.0	0.39448714698248893	1.2458800304742255E-4
30	4.152933434914085E-5	2.0764667174570425E-5	0.0	0.6345889935220468	1.2458800304742255E-4
31	4.152933434914085E-5	2.0764667174570425E-5	0.0	1.2176193184496351	1.2458800304742255E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCT	100	5.885626E-6	16.650002	4
CTTATAC	6680	0.0	15.952096	37
TACTTAC	1755	0.0	14.757834	31
TCGTTAC	815	0.0	14.754601	23
GGAGTGT	1955	0.0	14.667519	6
GTACTAA	320	0.0	14.453125	1
TTAGTAC	325	0.0	14.23077	3
AGTGTAC	2225	0.0	14.2179785	27
TAGATCG	170	2.442357E-8	14.147059	5
AACGCAG	21290	0.0	13.7902775	5
CATTACG	745	0.0	13.409395	24
TATACAC	2890	0.0	13.378892	37
TAAGACT	820	0.0	13.310975	4
CTCGTTA	1015	0.0	13.305419	22
TAGACAG	870	0.0	13.1839075	5
ATTACGG	730	0.0	13.178082	25
GAGTGTC	1885	0.0	13.151194	7
ACAGCTA	2435	0.0	13.143738	19
CTACAGT	2475	0.0	12.931313	23
TAATACG	145	1.3740157E-5	12.75862	4
>>END_MODULE