FastQCFastQC Report
Thu 9 Feb 2017
SRR2727409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4394647
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT344710.7843860951744247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT139040.31638491100650407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT133150.30298224180463185No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88140.20056218394788025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC59100.017.96785237
AACGCAG142300.017.199935
ACGCAGA159850.015.3809826
CGCAGAG163600.015.09627157
AGAGTAC167550.014.65204410
CAGAGTA170900.014.4839099
GCAGAGT174050.014.2855518
GGACCGT2800.013.8750016
CGATAAA1753.5735866E-813.74285725
GGTATCA91850.013.5955351
TCGTTAC2457.2759576E-1213.59183623
TAAGACT7350.013.5918364
TATACAC23450.013.41151437
CAACGCA187400.013.1296694
TCCGTAT1703.738296E-713.0588248
ATCAACG188000.013.0484052
TCAACGC189150.013.04731753
GTCCTAC5450.012.8990821
TATCAAC192650.012.7046461
TGGACCG4250.012.62352855
Produced by FastQC (version 0.11.5)