##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727409.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4394647
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29727757428526	33.0	31.0	34.0	30.0	34.0
2	32.44509741055425	34.0	31.0	34.0	31.0	34.0
3	32.53959692325686	34.0	31.0	34.0	31.0	34.0
4	36.08007855921078	37.0	35.0	37.0	35.0	37.0
5	35.890002541728606	37.0	35.0	37.0	35.0	37.0
6	35.90413905826794	37.0	35.0	37.0	35.0	37.0
7	35.8780600580661	37.0	35.0	37.0	35.0	37.0
8	35.83027191945109	37.0	35.0	37.0	35.0	37.0
9	37.66637161073461	39.0	37.0	39.0	35.0	39.0
10	37.49244842646065	39.0	37.0	39.0	35.0	39.0
11	37.59987707772661	39.0	37.0	39.0	35.0	39.0
12	37.525777383257406	39.0	37.0	39.0	35.0	39.0
13	37.60525066063327	39.0	37.0	39.0	35.0	39.0
14	38.82461640263712	40.0	38.0	41.0	35.0	41.0
15	38.749552580673715	40.0	38.0	41.0	35.0	41.0
16	38.72224936382831	40.0	38.0	41.0	35.0	41.0
17	38.66771324295217	40.0	38.0	41.0	35.0	41.0
18	38.63885950339129	40.0	38.0	41.0	34.0	41.0
19	38.69514434265141	40.0	38.0	41.0	34.0	41.0
20	38.657987092023546	40.0	38.0	41.0	34.0	41.0
21	38.61817160741238	40.0	38.0	41.0	34.0	41.0
22	38.57107999800667	40.0	38.0	41.0	34.0	41.0
23	38.51071906344241	40.0	38.0	41.0	34.0	41.0
24	38.46982408370911	40.0	38.0	41.0	34.0	41.0
25	38.36406246053437	40.0	38.0	41.0	34.0	41.0
26	38.25775085006828	40.0	38.0	41.0	34.0	41.0
27	38.17259383973275	40.0	38.0	41.0	34.0	41.0
28	38.085978691803916	40.0	38.0	41.0	33.0	41.0
29	38.0079339705783	40.0	37.0	41.0	33.0	41.0
30	37.942120265859806	40.0	37.0	41.0	33.0	41.0
31	37.85035840193763	40.0	37.0	41.0	33.0	41.0
32	37.75698184632349	40.0	37.0	41.0	33.0	41.0
33	37.662828664054246	40.0	37.0	41.0	33.0	41.0
34	37.56669580059559	40.0	37.0	41.0	33.0	41.0
35	37.50216638560503	40.0	37.0	41.0	33.0	41.0
36	37.42272860596084	40.0	37.0	41.0	32.0	41.0
37	37.34402217060892	40.0	37.0	41.0	32.0	41.0
38	37.25520912146072	40.0	36.0	41.0	32.0	41.0
39	37.1549075500262	39.0	36.0	41.0	31.0	41.0
40	37.039926756346986	39.0	36.0	41.0	31.0	41.0
41	36.93570700900436	39.0	36.0	41.0	31.0	41.0
42	36.82403728900182	39.0	36.0	41.0	31.0	41.0
43	35.178767714448966	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	9.0
11	22.0
12	11.0
13	5.0
14	8.0
15	13.0
16	18.0
17	47.0
18	103.0
19	211.0
20	444.0
21	941.0
22	1766.0
23	3283.0
24	5671.0
25	9264.0
26	14187.0
27	21457.0
28	31375.0
29	43857.0
30	59818.0
31	78713.0
32	101717.0
33	134381.0
34	182635.0
35	251735.0
36	372637.0
37	623173.0
38	1196190.0
39	1260948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41436297386343	18.192632991910386	12.756291916051504	25.63671211817468
2	16.755657507872645	21.346902265415174	36.877614971122824	25.019825255589357
3	18.099678995832885	22.713633199663136	31.39954130559292	27.787146498911063
4	13.01692718436771	15.672953936914617	36.253696827071664	35.05642205164602
5	14.016302105720893	37.43948034961625	35.017647606281	13.526569938381854
6	33.909503994291235	37.10889634594087	15.262591056801606	13.71900860296629
7	29.07905913717302	31.365954990241534	21.496061003306977	18.058924869278464
8	27.15351198856245	36.085378416059356	19.626104212693306	17.13500538268489
9	26.492343981211686	13.732911881204565	19.184430512848927	40.59031362473482
10	14.745302637504217	27.989324284749152	35.09333059060261	22.17204248714402
11	36.099509243859636	21.46709394406422	22.600336272742723	19.83306053933342
12	20.736477810390685	25.921080805807613	31.728987561458293	21.61345382234341
13	31.229334233216	19.569922225835203	25.067565153697213	24.13317838725158
14	21.330382167213884	20.08382015665877	27.935167488992857	30.650630187134485
15	24.48804192919249	28.593627656555803	22.955131549815036	23.963198864436666
16	23.54614602720082	26.734183655706588	25.42943722214776	24.290233094944828
17	23.395098627944407	27.011475551961283	26.644256068803706	22.949169751290604
18	23.289288081613833	26.18531135720343	27.24175570870652	23.283644852476208
19	24.442395487055045	26.05610871589914	27.240822755502318	22.260673041543498
20	23.808169347845233	26.147856699298032	27.073255258044615	22.97071869481212
21	23.486163962657297	26.304023963699475	27.204414825582123	23.005397248061108
22	23.81501858966147	25.89828033969509	26.969492657772058	23.317208412871388
23	23.226370627720495	25.96950335260147	27.100538450528568	23.703587569149466
24	23.162406445841953	26.10341626983919	27.221230738214015	23.51294654610484
25	23.58553485638323	25.654119659667774	27.11633039013145	23.644015093817547
26	23.166684377607577	26.055721881643734	27.461181751344306	23.316411989404383
27	23.537908733056376	26.434614657331977	26.908395600374728	23.11908100923692
28	23.234607921864942	26.30375090422507	27.236544823736697	23.225096350173292
29	23.149618160457482	26.697957765435998	27.156288093218862	22.996135980887658
30	23.26928647511393	26.631900127587038	26.903412264966903	23.19540113233213
31	23.29545467474407	25.779340183637046	27.22209542654962	23.703109715069264
32	22.696430452775843	26.367396516716813	27.35751017089655	23.578662859610795
33	23.148457657691278	25.79667946026154	27.328520356697588	23.72634252534959
34	23.179518172904444	25.709459713146472	27.9614722183602	23.149549895588883
35	23.01716156041657	25.781547414388463	27.623629383656983	23.57766164153799
36	22.806291381310036	25.555727229058444	27.9243133748854	23.713668014746123
37	22.676269561582536	25.00660462603709	27.958809888484787	24.358315923895592
38	22.266111476075327	25.04146521893567	28.58684667960817	24.105576625380834
39	22.246155379487817	24.814712080401453	29.021033998862706	23.91809854124802
40	22.25145728428245	24.84342883512601	29.280258459894505	23.624855420697045
41	21.839433292366827	24.603705371557716	29.668093933369395	23.888767402706065
42	21.348540622261584	25.002372204183864	29.45615427132145	24.192932902233103
43	21.046559598529758	24.32033790199759	29.53060848800825	25.102494011464405
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	866.0
1	1041.5
2	1217.0
3	4049.5
4	6882.0
5	6882.0
6	8706.0
7	10530.0
8	9462.5
9	8395.0
10	11090.0
11	13785.0
12	13785.0
13	20613.0
14	27441.0
15	43623.0
16	59805.0
17	48566.5
18	37328.0
19	37328.0
20	40532.5
21	43737.0
22	38571.5
23	33406.0
24	40153.5
25	46901.0
26	46901.0
27	56941.0
28	66981.0
29	77349.5
30	87718.0
31	100828.5
32	113939.0
33	113939.0
34	130511.0
35	147083.0
36	166466.5
37	185850.0
38	207581.0
39	229312.0
40	229312.0
41	250461.5
42	271611.0
43	291762.0
44	311913.0
45	329219.5
46	346526.0
47	346526.0
48	360495.5
49	374465.0
50	378323.5
51	382182.0
52	370854.5
53	359527.0
54	359527.0
55	338006.0
56	316485.0
57	290568.0
58	264651.0
59	237795.5
60	210940.0
61	210940.0
62	182727.5
63	154515.0
64	130025.0
65	105535.0
66	87237.5
67	68940.0
68	68940.0
69	55567.0
70	42194.0
71	34197.5
72	26201.0
73	21036.0
74	15871.0
75	15871.0
76	12737.0
77	9603.0
78	7653.5
79	5704.0
80	4417.0
81	3130.0
82	3130.0
83	2500.5
84	1871.0
85	1393.0
86	915.0
87	683.0
88	451.0
89	451.0
90	296.0
91	141.0
92	101.0
93	61.0
94	45.5
95	30.0
96	30.0
97	18.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4394647.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.94196753163057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.42836333967753	50.6080221405566
2	8.118343505038228	9.73258965579251
3	2.650571785798284	4.766414637737304
4	1.3175421882793084	3.1590428428556723
5	0.778099974156393	2.3320421693622544
6	0.4953317648557049	1.781469633981956
7	0.34862055643561923	1.4627901452306011
8	0.2680025497829922	1.285168010998906
9	0.20532532488200156	1.1076843561748593
>10	1.2632202672454855	14.225796765770735
>50	0.08986098265395497	3.6570691031379243
>100	0.03569455984946048	3.5726209785201934
>500	5.305489059600444E-4	0.21754937946196215
>1k	3.4106707316470636E-4	0.4901845221512636
>5k	3.789634146273617E-5	0.20021717309610249
>10k+	1.1368902438820852E-4	1.4013384851711552
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34471	0.7843860951744247	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13904	0.31638491100650407	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13315	0.30298224180463185	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8814	0.20056218394788025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2754956200122557E-5	0.0
2	0.0	0.0	0.0	2.2754956200122557E-5	0.0
3	0.0	0.0	0.0	2.2754956200122557E-5	0.0
4	0.0	0.0	0.0	2.2754956200122557E-5	0.0
5	0.0	0.0	0.0	2.2754956200122557E-5	0.0
6	4.550991240024511E-5	0.0	0.0	2.2754956200122557E-5	0.0
7	4.550991240024511E-5	0.0	0.0	2.2754956200122557E-5	0.0
8	4.550991240024511E-5	0.0	0.0	4.550991240024511E-5	2.2754956200122557E-5
9	4.550991240024511E-5	0.0	0.0	4.550991240024511E-5	6.826486860036768E-5
10	4.550991240024511E-5	0.0	0.0	6.826486860036768E-5	6.826486860036768E-5
11	4.550991240024511E-5	0.0	0.0	1.592846934008579E-4	6.826486860036768E-5
12	4.550991240024511E-5	0.0	0.0	2.730594744014707E-4	6.826486860036768E-5
13	4.550991240024511E-5	0.0	0.0	3.185693868017158E-4	6.826486860036768E-5
14	4.550991240024511E-5	0.0	0.0	3.4132434300183836E-4	2.0479460580110303E-4
15	4.550991240024511E-5	0.0	0.0	4.7785408020257374E-4	2.730594744014707E-4
16	4.550991240024511E-5	0.0	0.0	7.73668510804167E-4	3.185693868017158E-4
17	4.550991240024511E-5	0.0	0.0	0.0010012180728053925	3.185693868017158E-4
18	4.550991240024511E-5	0.0	0.0	0.001183257722406373	3.4132434300183836E-4
19	4.550991240024511E-5	0.0	0.0	0.0017293766712093144	3.4132434300183836E-4
20	4.550991240024511E-5	0.0	0.0	0.0025485550944137265	3.4132434300183836E-4
21	9.101982480049023E-5	0.0	0.0	0.0036180380358194868	3.4132434300183836E-4
22	9.101982480049023E-5	0.0	0.0	0.006780976947636522	3.4132434300183836E-4
23	9.101982480049023E-5	0.0	0.0	0.008942697786648166	5.233639926028188E-4
24	9.101982480049023E-5	0.0	0.0	0.012515225910067406	5.461189488029414E-4
25	9.101982480049023E-5	0.0	0.0	0.014608681880478683	5.461189488029414E-4
26	9.101982480049023E-5	0.0	0.0	0.017384786536893633	5.688739050030639E-4
27	9.101982480049023E-5	0.0	0.0	0.03406416943158347	5.688739050030639E-4
28	9.101982480049023E-5	0.0	0.0	0.14770242069499553	5.688739050030639E-4
29	9.101982480049023E-5	2.2754956200122557E-5	0.0	0.3325636848647912	5.688739050030639E-4
30	9.101982480049023E-5	2.2754956200122557E-5	0.0	0.5722871484330824	5.688739050030639E-4
31	9.101982480049023E-5	2.2754956200122557E-5	0.0	1.2395762390016765	5.688739050030639E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5910	0.0	17.967852	37
AACGCAG	14230	0.0	17.19993	5
ACGCAGA	15985	0.0	15.380982	6
CGCAGAG	16360	0.0	15.0962715	7
AGAGTAC	16755	0.0	14.652044	10
CAGAGTA	17090	0.0	14.483909	9
GCAGAGT	17405	0.0	14.285551	8
GGACCGT	280	0.0	13.875001	6
CGATAAA	175	3.5735866E-8	13.742857	25
GGTATCA	9185	0.0	13.595535	1
TCGTTAC	245	7.2759576E-12	13.591836	23
TAAGACT	735	0.0	13.591836	4
TATACAC	2345	0.0	13.411514	37
CAACGCA	18740	0.0	13.129669	4
TCCGTAT	170	3.738296E-7	13.058824	8
ATCAACG	18800	0.0	13.048405	2
TCAACGC	18915	0.0	13.0473175	3
GTCCTAC	545	0.0	12.899082	1
TATCAAC	19265	0.0	12.704646	1
TGGACCG	425	0.0	12.6235285	5
>>END_MODULE