FastQCFastQC Report
Thu 9 Feb 2017
SRR2727408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4727874
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT264490.5594269221218671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111880.23663913209192972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104260.2205219513041168No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65610.13877273379112895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC43800.016.89497837
AACGCAG105750.015.9196215
GTATACG1659.767973E-1015.696971
TAGGTCG2151.2732926E-1114.6279075
GTACCGT3800.014.6052646
CTAAGAC7400.014.53
GGTATCA64450.014.4670291
ACGCAGA119450.014.1247396
TAGATCG1601.7869752E-713.8755
CGCGTAT800.00630244913.87521
CGCAGAG123850.013.6079937
ATTATAC6800.013.6029413
AGAGTAC128800.013.17119610
TAGACTG7250.013.0137935
CAGAGTA132750.012.9604539
GTCTTAG9050.012.8784531
TTATACT9950.012.8291454
GTATAGG6500.012.8076931
GCAGAGT134900.012.6990378
TATACTG7150.012.6783225
Produced by FastQC (version 0.11.5)