FastQCFastQC Report
Thu 9 Feb 2017
SRR2727407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257195
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT160290.7101291647376501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66330.29386030006268843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60740.26909504938651735No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43370.19214113091691237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG951.6779086E-719.4736845
CTCGACG701.2198583E-418.531
AATACCG500.007036932718.4999985
CTTATAC23850.018.15094437
ATACCGT904.4490123E-516.4444456
TACCGTC1251.6596823E-716.2799997
GTCTTAG3400.015.7794121
GTCTTAT3050.015.7704931
TAAGACT3550.015.6338024
AACGCAG65300.015.4969375
TCGACGA855.366995E-415.23529432
TCGTTAC1853.0559022E-1015.023
TACGCTT750.00410603514.85
ATATACT2950.014.423734
TAATACG908.278372E-414.3888884
CGACCGA908.278372E-414.38888818
TAGATCG908.278372E-414.3888885
GTATAGA3350.014.358211
ACGCAGA72150.014.1025646
TATACTC2101.364242E-1014.0952395
Produced by FastQC (version 0.11.5)