##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727407.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2257195
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39463847828832	33.0	31.0	34.0	31.0	34.0
2	32.53578844539351	34.0	31.0	34.0	31.0	34.0
3	32.62596452676885	34.0	31.0	34.0	31.0	34.0
4	36.146841987511046	37.0	35.0	37.0	35.0	37.0
5	35.95895170776118	37.0	35.0	37.0	35.0	37.0
6	35.97475362119799	37.0	35.0	37.0	35.0	37.0
7	35.94050757688193	37.0	35.0	37.0	35.0	37.0
8	35.89601828818512	37.0	35.0	37.0	35.0	37.0
9	37.739791644053795	39.0	37.0	39.0	35.0	39.0
10	37.574596346350226	39.0	37.0	39.0	35.0	39.0
11	37.680993888432326	39.0	37.0	39.0	35.0	39.0
12	37.60888625041257	39.0	37.0	39.0	35.0	39.0
13	37.68843409630094	39.0	37.0	39.0	35.0	39.0
14	38.93681804186169	40.0	38.0	41.0	36.0	41.0
15	38.86586803532703	40.0	38.0	41.0	36.0	41.0
16	38.83718686245539	40.0	38.0	41.0	35.0	41.0
17	38.77719824826831	40.0	38.0	41.0	35.0	41.0
18	38.73910849527843	40.0	38.0	41.0	35.0	41.0
19	38.78755047747315	40.0	38.0	41.0	35.0	41.0
20	38.75271742140134	40.0	38.0	41.0	35.0	41.0
21	38.71459709949738	40.0	38.0	41.0	35.0	41.0
22	38.67677803645675	40.0	38.0	41.0	35.0	41.0
23	38.61470453372438	40.0	38.0	41.0	34.0	41.0
24	38.57348611883333	40.0	38.0	41.0	34.0	41.0
25	38.46994521961992	40.0	38.0	41.0	34.0	41.0
26	38.36858756111014	40.0	38.0	41.0	34.0	41.0
27	38.2859518118727	40.0	38.0	41.0	34.0	41.0
28	38.203767507902505	40.0	38.0	41.0	34.0	41.0
29	38.123392972250954	40.0	38.0	41.0	34.0	41.0
30	38.06352796280339	40.0	37.0	41.0	33.0	41.0
31	37.97453343641112	40.0	37.0	41.0	33.0	41.0
32	37.88093452271514	40.0	37.0	41.0	33.0	41.0
33	37.788583175135514	40.0	37.0	41.0	33.0	41.0
34	37.70213029888866	40.0	37.0	41.0	33.0	41.0
35	37.63035847589597	40.0	37.0	41.0	33.0	41.0
36	37.54710248782227	40.0	37.0	41.0	33.0	41.0
37	37.468853599268115	40.0	37.0	41.0	32.0	41.0
38	37.378150314881964	40.0	37.0	41.0	32.0	41.0
39	37.27297109908537	40.0	36.0	41.0	32.0	41.0
40	37.153234434774134	39.0	36.0	41.0	31.0	41.0
41	37.0478828811866	39.0	36.0	41.0	31.0	41.0
42	36.93856800143541	39.0	36.0	41.0	31.0	41.0
43	35.292804564957834	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	5.0
12	1.0
13	1.0
14	2.0
15	4.0
16	14.0
17	15.0
18	47.0
19	82.0
20	216.0
21	410.0
22	787.0
23	1410.0
24	2553.0
25	4108.0
26	6514.0
27	9851.0
28	14602.0
29	20815.0
30	28304.0
31	37185.0
32	48958.0
33	65136.0
34	89320.0
35	124809.0
36	187534.0
37	314952.0
38	618374.0
39	681181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.13021471339428	17.894776481429382	12.291937559670298	25.68307124550604
2	16.79615629132618	20.96832573171569	36.77670737353219	25.458810603425935
3	18.171801727365157	22.652539988791396	31.079060515374167	28.096597768469273
4	13.150126595176756	15.128068243993098	35.886930460150765	35.83487470067938
5	14.128154634402435	37.439875597810556	34.95852152782547	13.473448239961543
6	34.90686449332025	36.46680946927492	14.950812845146299	13.675513192258535
7	29.604309773856492	31.229911460906123	21.306090080830412	17.859688684406976
8	27.619811314485453	36.07127430284047	19.300281987156627	17.008632395517445
9	26.77358402796391	13.49839070173379	18.72058018912854	41.00744508117376
10	14.721413081280085	27.668544365905472	34.959008858339665	22.65103369447478
11	36.64760909004317	21.208978400182527	22.248587295293497	19.894825214480804
12	20.865720507089552	25.171019783403736	31.854137546822493	22.109122162684216
13	31.389711566789753	19.205739867401796	25.051269385232555	24.353279180575893
14	21.457472659650584	19.50190391171343	27.55769882531195	31.482924603324037
15	24.78558564944544	28.37357871163103	22.676419183987207	24.164416454936326
16	24.01068582909319	26.476844047590042	24.945474360877107	24.566995762439664
17	23.725686083834137	26.77163470590711	26.29963295151726	23.20304625874149
18	23.589809475920333	26.008519423443698	26.86320853980272	23.538462560833246
19	24.610412480977498	25.975425251252105	26.83919643628486	22.57496583148554
20	23.90883375162536	25.889787989074936	26.7886469711301	23.41273128816961
21	23.593973936678044	26.14085180943605	26.9215995959587	23.343574657927206
22	24.036071318605615	25.570409291177764	26.532001001242694	23.861518388973927
23	23.40488969716839	25.73277895795445	26.741553122348755	24.1207782225284
24	23.43253462815574	25.746645726222145	26.859664317881265	23.961155327740848
25	23.865284124765473	25.53922013826896	26.70238947011667	23.8931062668489
26	23.456591034447623	25.894882808087026	26.986458857121338	23.66206730034401
27	23.843664371044593	26.32227166904056	26.466122776277633	23.367941183637214
28	23.625428906230965	26.293341957606675	26.56279142918534	23.51843770697702
29	23.50479245257942	26.513039413962908	26.741818938992868	23.240349194464812
30	23.72417978951752	26.429838804356738	26.489691852055312	23.35628955407043
31	23.586176648450845	25.721526053353834	26.843892530330788	23.84840476786454
32	23.119801346361303	26.328429754629084	26.776906736015277	23.77486216299434
33	23.392839342635437	25.867237877099676	26.747179574649067	23.992743205615817
34	23.58174637104902	25.82231486424523	27.273629438307278	23.32230932639847
35	23.41463630745239	25.88296536187613	26.984066507324357	23.718331823347118
36	23.21757756861946	25.694678572298805	27.220820531677592	23.866923327404148
37	23.106200394737716	25.245980077042525	27.27482561320577	24.37299391501399
38	22.87223744514763	25.319699893008803	27.703011924091626	24.10505073775194
39	22.733835579114785	25.188297865270837	28.069218654126026	24.008647901488352
40	23.02481619886629	25.02464341804762	28.240360270158316	23.71018011292777
41	22.541384328779746	24.938518825356248	28.489519071236646	24.03057777462736
42	22.140532829463115	25.157551740102207	28.389128985311412	24.312786445123262
43	21.82841978650493	24.61023526988142	28.46027924038464	25.101065703229008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	376.0
1	451.0
2	526.0
3	1915.5
4	3305.0
5	3305.0
6	4232.5
7	5160.0
8	4588.0
9	4016.0
10	5269.5
11	6523.0
12	6523.0
13	10097.0
14	13671.0
15	21276.0
16	28881.0
17	23719.0
18	18557.0
19	18557.0
20	20208.5
21	21860.0
22	19083.5
23	16307.0
24	19323.5
25	22340.0
26	22340.0
27	26951.0
28	31562.0
29	36604.0
30	41646.0
31	47428.5
32	53211.0
33	53211.0
34	61096.0
35	68981.0
36	78583.5
37	88186.0
38	99108.0
39	110030.0
40	110030.0
41	121184.0
42	132338.0
43	143532.0
44	154726.0
45	164992.5
46	175259.0
47	175259.0
48	184557.0
49	193855.0
50	196534.5
51	199214.0
52	195633.0
53	192052.0
54	192052.0
55	181807.0
56	171562.0
57	158028.5
58	144495.0
59	130882.5
60	117270.0
61	117270.0
62	101672.0
63	86074.0
64	72964.5
65	59855.0
66	48988.0
67	38121.0
68	38121.0
69	30746.5
70	23372.0
71	18829.0
72	14286.0
73	11465.0
74	8644.0
75	8644.0
76	6833.0
77	5022.0
78	3934.0
79	2846.0
80	2187.0
81	1528.0
82	1528.0
83	1171.5
84	815.0
85	625.5
86	436.0
87	306.0
88	176.0
89	176.0
90	122.5
91	69.0
92	45.5
93	22.0
94	18.5
95	15.0
96	15.0
97	9.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2257195.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.04027910964105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.92278107087854	49.787069200432065
2	8.845466943214268	10.621686082513762
3	2.9563361997010635	5.324977517159621
4	1.4092357102485675	3.3844362149838894
5	0.8513359709879053	2.5557224657095556
6	0.5809029814520288	2.092654628520147
7	0.4136268205511671	1.738398882718784
8	0.31255706306004616	1.501281064305188
9	0.24093221431582906	1.3019073654623607
>10	1.374292565860614	14.91103589160672
>50	0.07039408684798365	2.852671860423062
>100	0.021180430825806094	2.0002523920461837
>500	3.684392523291956E-4	0.1489641307986115
>1k	3.6843925232898954E-4	0.5075929529705219
>5k	1.4737570093159582E-4	0.5621880635750389
>10k+	7.368785046579791E-5	0.7091612867745573
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16029	0.7101291647376501	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6633	0.29386030006268843	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6074	0.26909504938651735	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4337	0.19214113091691237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.430277401819515E-5
2	0.0	0.0	0.0	0.0	4.430277401819515E-5
3	0.0	0.0	0.0	0.0	4.430277401819515E-5
4	0.0	0.0	0.0	0.0	4.430277401819515E-5
5	0.0	0.0	0.0	0.0	4.430277401819515E-5
6	0.0	0.0	0.0	0.0	4.430277401819515E-5
7	0.0	0.0	0.0	0.0	4.430277401819515E-5
8	0.0	0.0	0.0	0.0	4.430277401819515E-5
9	0.0	0.0	0.0	0.0	8.86055480363903E-5
10	0.0	0.0	0.0	0.0	8.86055480363903E-5
11	0.0	0.0	0.0	8.86055480363903E-5	8.86055480363903E-5
12	0.0	0.0	0.0	1.772110960727806E-4	8.86055480363903E-5
13	0.0	0.0	0.0	2.2151387009097575E-4	8.86055480363903E-5
14	0.0	0.0	0.0	2.658166441091709E-4	8.86055480363903E-5
15	0.0	0.0	0.0	3.544221921455612E-4	1.3290832205458544E-4
16	0.0	0.0	0.0	6.645416102729273E-4	1.3290832205458544E-4
17	0.0	0.0	0.0	9.746610284002933E-4	1.3290832205458544E-4
18	4.430277401819515E-5	0.0	0.0	0.0010632665764366835	1.772110960727806E-4
19	4.430277401819515E-5	0.0	0.0	0.0015505970906368302	1.772110960727806E-4
20	4.430277401819515E-5	0.0	0.0	0.0024809553450189283	1.772110960727806E-4
21	4.430277401819515E-5	0.0	0.0	0.003987249661637563	1.772110960727806E-4
22	4.430277401819515E-5	0.0	0.0	0.007664379905147761	1.772110960727806E-4
23	4.430277401819515E-5	0.0	0.0	0.009436490865875566	3.544221921455612E-4
24	4.430277401819515E-5	0.0	0.0	0.013822465493676887	3.544221921455612E-4
25	4.430277401819515E-5	0.0	0.0	0.017676806833259864	3.544221921455612E-4
26	4.430277401819515E-5	0.0	0.0	0.02091090933658811	3.544221921455612E-4
27	4.430277401819515E-5	0.0	0.0	0.03273974999944622	3.544221921455612E-4
28	4.430277401819515E-5	0.0	0.0	0.10832028247448713	3.544221921455612E-4
29	4.430277401819515E-5	0.0	0.0	0.2409184851109452	3.544221921455612E-4
30	4.430277401819515E-5	0.0	0.0	0.4063893460689041	3.544221921455612E-4
31	4.430277401819515E-5	0.0	0.0	0.8666951681179517	3.544221921455612E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	95	1.6779086E-7	19.473684	5
CTCGACG	70	1.2198583E-4	18.5	31
AATACCG	50	0.0070369327	18.499998	5
CTTATAC	2385	0.0	18.150944	37
ATACCGT	90	4.4490123E-5	16.444445	6
TACCGTC	125	1.6596823E-7	16.279999	7
GTCTTAG	340	0.0	15.779412	1
GTCTTAT	305	0.0	15.770493	1
TAAGACT	355	0.0	15.633802	4
AACGCAG	6530	0.0	15.496937	5
TCGACGA	85	5.366995E-4	15.235294	32
TCGTTAC	185	3.0559022E-10	15.0	23
TACGCTT	75	0.004106035	14.8	5
ATATACT	295	0.0	14.42373	4
TAATACG	90	8.278372E-4	14.388888	4
CGACCGA	90	8.278372E-4	14.388888	18
TAGATCG	90	8.278372E-4	14.388888	5
GTATAGA	335	0.0	14.35821	1
ACGCAGA	7215	0.0	14.102564	6
TATACTC	210	1.364242E-10	14.095239	5
>>END_MODULE