FastQCFastQC Report
Thu 9 Feb 2017
SRR2727406.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727406.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835418
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT260940.9202875907538148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103140.363755890665856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96210.3393150498445026No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68040.2399646189732872No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40590.1431534962393552No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40440.14262447371075446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1352.2231688E-816.4444435
TAGATCG1059.352694E-615.8571425
CTTATAC39200.015.38520337
TCTATAC3100.014.9193553
GTATTAG4150.014.7108431
AACGCAG112750.014.5374735
GCACCGT3500.014.2714286
ACCGTGC3400.014.1470598
GTACCGT1854.9076334E-914.06
TCGTATA800.006301626613.8752
TAATACC3100.013.7258074
TCGTTAC2700.013.70370323
ATTGCGA1907.1377144E-913.63157910
ACGCGCA1102.4583534E-413.45454519
TAAGACT5100.013.0588244
ACGCAGA126200.013.0320926
TAGACTA2001.4650141E-812.9499995
CGACGCG1153.5800505E-412.86956517
CGCAGAG127800.012.8689357
CGATTCA1307.004869E-512.80769314
Produced by FastQC (version 0.11.5)