##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727406.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2835418
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32489142694305	33.0	31.0	34.0	30.0	34.0
2	32.466081896919604	34.0	31.0	34.0	31.0	34.0
3	32.56086862677743	34.0	31.0	34.0	31.0	34.0
4	36.09325644402342	37.0	35.0	37.0	35.0	37.0
5	35.90647551789542	37.0	35.0	37.0	35.0	37.0
6	35.92610542784168	37.0	35.0	37.0	35.0	37.0
7	35.894861709984205	37.0	35.0	37.0	35.0	37.0
8	35.84868756564288	37.0	35.0	37.0	35.0	37.0
9	37.68183280207715	39.0	37.0	39.0	35.0	39.0
10	37.52448810016724	39.0	37.0	39.0	35.0	39.0
11	37.62659685450258	39.0	37.0	39.0	35.0	39.0
12	37.55600514633116	39.0	37.0	39.0	35.0	39.0
13	37.63603285300439	39.0	37.0	39.0	35.0	39.0
14	38.85590872315828	40.0	38.0	41.0	36.0	41.0
15	38.785975119012434	40.0	38.0	41.0	35.0	41.0
16	38.760540068519	40.0	38.0	41.0	35.0	41.0
17	38.69798632864713	40.0	38.0	41.0	35.0	41.0
18	38.67549581754789	40.0	38.0	41.0	35.0	41.0
19	38.72905370566174	40.0	38.0	41.0	35.0	41.0
20	38.6916535762981	40.0	38.0	41.0	34.0	41.0
21	38.64768192908418	40.0	38.0	41.0	34.0	41.0
22	38.60538375646907	40.0	38.0	41.0	34.0	41.0
23	38.533377794737845	40.0	38.0	41.0	34.0	41.0
24	38.49661919336056	40.0	38.0	41.0	34.0	41.0
25	38.38658991372701	40.0	38.0	41.0	34.0	41.0
26	38.28008392413394	40.0	38.0	41.0	34.0	41.0
27	38.19351714632552	40.0	38.0	41.0	34.0	41.0
28	38.103873220809064	40.0	38.0	41.0	33.0	41.0
29	38.01679505455633	40.0	37.0	41.0	33.0	41.0
30	37.938764936951095	40.0	37.0	41.0	33.0	41.0
31	37.83766344151021	40.0	37.0	41.0	33.0	41.0
32	37.73953963754198	40.0	37.0	41.0	33.0	41.0
33	37.630355735909134	40.0	37.0	41.0	33.0	41.0
34	37.526486747280295	40.0	37.0	41.0	33.0	41.0
35	37.45332786911842	40.0	37.0	41.0	33.0	41.0
36	37.35500797413292	40.0	36.0	41.0	32.0	41.0
37	37.271291569708595	40.0	36.0	41.0	32.0	41.0
38	37.16697255924876	40.0	36.0	41.0	31.0	41.0
39	37.06210265999581	39.0	36.0	41.0	31.0	41.0
40	36.936838942265304	39.0	36.0	41.0	31.0	41.0
41	36.82376425627544	39.0	36.0	41.0	31.0	41.0
42	36.700861389749235	39.0	36.0	41.0	30.0	41.0
43	35.07482988398888	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	0.0
10	9.0
11	8.0
12	10.0
13	9.0
14	8.0
15	11.0
16	18.0
17	24.0
18	96.0
19	132.0
20	271.0
21	629.0
22	1176.0
23	2123.0
24	3752.0
25	5915.0
26	9629.0
27	14182.0
28	20895.0
29	29203.0
30	39520.0
31	50913.0
32	65492.0
33	86193.0
34	116832.0
35	161127.0
36	239288.0
37	401141.0
38	765061.0
39	821747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.286774648393994	18.699465122955417	13.283755693164112	24.730004535486476
2	16.519680696109003	21.741873684938167	37.32426753304098	24.414178085911846
3	18.093452182359005	23.383395323017627	31.435435621837765	27.0877168727856
4	13.337892331924253	15.628489344428228	35.933855255203994	35.09976306844352
5	14.198611985957626	37.45927408233989	34.76429224897352	13.577821682728967
6	33.77777103763889	36.85354328709206	15.38570327196907	13.982982403299973
7	28.558611111307048	31.42654804335728	22.002858132381185	18.01198271295449
8	26.82881324728841	36.3629983304049	20.016731219171213	16.791457203135483
9	26.23359236627545	14.113509895190058	19.586424294407383	40.06647344412711
10	14.949259685873475	27.92244388658039	34.955727867989836	22.1725685595563
11	35.70937336223442	21.535519630615312	22.849047300962326	19.906059706187943
12	20.58578311910272	25.906268493745895	31.945766021094595	21.56218236605679
13	30.97289359099787	19.572175954303738	25.558206938095196	23.896723516603195
14	21.237856287855973	20.029286687183337	28.300800799035624	30.432056225925063
15	24.493214051684795	28.683354623551093	23.25533660292768	23.568094721836427
16	23.510395998050377	26.66834308027952	25.515532454121402	24.3057284675487
17	23.190337368246936	27.153668348017824	27.127675707779243	22.528318575955996
18	23.22507651429172	26.132866476829868	27.63698333014744	23.005073678730966
19	24.009123169846564	26.321445374191743	27.77643366868659	21.892997787275103
20	23.42733946106006	26.304869334962248	27.547296377465337	22.720494826512354
21	23.027821647460797	26.702094717604247	27.59776512669384	22.672318508241112
22	23.49741731201537	26.091990669453324	27.244730759274294	23.16586125925701
23	22.88681950950442	26.022265500183746	27.476618967644278	23.61429602266756
24	22.988779784850063	26.165630605434544	27.615222870137668	23.230366739577725
25	23.3263314262659	25.888740213964923	27.433309656636162	23.351618703133013
26	22.89672986487354	26.269001607523123	27.895463737621757	22.93880478998158
27	23.436297575877703	26.56430198298805	27.366970231549637	22.63243020958462
28	23.09634769899888	26.582747235151928	27.503175898580036	22.817729167269167
29	22.968676928763237	26.754855897790026	27.58083640577862	22.695630767668117
30	23.027292624932198	26.64009327725224	27.447205315054074	22.88540878276148
31	23.019956845868933	26.066280174563328	27.604254469711343	23.309508509856393
32	22.464165777321014	26.39681345043306	27.71965191728345	23.419368854962478
33	22.91655057561178	26.0521376389654	27.587784234987573	23.443527550435245
34	23.01660636985446	25.981248620132902	28.134370311537843	22.867774698474793
35	22.836668173793072	26.029953960932744	27.904562925113684	23.228814940160497
36	22.68833025677343	25.849839424028488	28.129468036106143	23.332362283091946
37	22.40664339437783	25.44541933499752	28.20444816249315	23.943489108131498
38	22.22307257695338	25.477372295725004	28.73971315693136	23.559841970390256
39	22.10157373621808	25.30159574355527	28.982181815873354	23.614648704353293
40	22.24666698172897	25.495641207046017	29.08036839718165	23.17732341404336
41	21.917826577950763	25.13283755693164	29.373623218869316	23.575712646248277
42	21.445726873427482	25.45342520926368	29.25455788176558	23.846290035543262
43	21.235422784224404	24.950183711890098	29.185326466856033	24.62906703702946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	985.0
1	1055.5
2	1126.0
3	4041.0
4	6956.0
5	6956.0
6	8358.0
7	9760.0
8	8558.0
9	7356.0
10	9292.5
11	11229.0
12	11229.0
13	16379.0
14	21529.0
15	33623.0
16	45717.0
17	37177.5
18	28638.0
19	28638.0
20	30493.0
21	32348.0
22	27651.0
23	22954.0
24	26951.0
25	30948.0
26	30948.0
27	37355.5
28	43763.0
29	50285.0
30	56807.0
31	64742.5
32	72678.0
33	72678.0
34	83207.0
35	93736.0
36	106367.0
37	118998.0
38	132794.5
39	146591.0
40	146591.0
41	160411.5
42	174232.0
43	186634.0
44	199036.0
45	210646.5
46	222257.0
47	222257.0
48	230644.5
49	239032.0
50	240250.0
51	241468.0
52	236354.5
53	231241.0
54	231241.0
55	217325.5
56	203410.0
57	184730.5
58	166051.0
59	149295.0
60	132539.0
61	132539.0
62	115010.0
63	97481.0
64	81631.5
65	65782.0
66	54418.0
67	43054.0
68	43054.0
69	35041.5
70	27029.0
71	22051.5
72	17074.0
73	13574.0
74	10074.0
75	10074.0
76	8112.5
77	6151.0
78	4814.5
79	3478.0
80	2679.5
81	1881.0
82	1881.0
83	1479.0
84	1077.0
85	818.5
86	560.0
87	404.0
88	248.0
89	248.0
90	169.0
91	90.0
92	63.5
93	37.0
94	25.0
95	13.0
96	13.0
97	7.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2835418.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.15130466565478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.8358805814151	48.99850409519081
2	9.016289198762754	10.666505386993363
3	2.919746450596117	5.181204355370252
4	1.4566290001124176	3.446460230819108
5	0.8544469162621912	2.5270824932227045
6	0.5568972950435859	1.9764720939961309
7	0.4003907581774594	1.6578545005587184
8	0.2906533580274527	1.3754020266221993
9	0.21852707192033913	1.163354526796809
>10	1.3446430426721732	14.421476992440407
>50	0.07721672291238417	3.098409612682781
>100	0.02707026981919721	2.643328667622726
>500	7.748646528068201E-4	0.2790953567567252
>1k	5.960497328573739E-4	0.7021102746189783
>5k	1.1920994657147478E-4	0.5790981852273134
>10k+	1.1920994657147478E-4	1.2836412010810365
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26094	0.9202875907538148	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10314	0.363755890665856	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9621	0.3393150498445026	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6804	0.2399646189732872	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4059	0.1431534962393552	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4044	0.14262447371075446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.526816857338142E-5	0.0	0.0	0.0
6	0.0	3.526816857338142E-5	0.0	0.0	0.0
7	0.0	3.526816857338142E-5	0.0	0.0	0.0
8	0.0	3.526816857338142E-5	0.0	0.0	0.0
9	0.0	3.526816857338142E-5	0.0	3.526816857338142E-5	1.4107267429352567E-4
10	0.0	3.526816857338142E-5	0.0	1.4107267429352567E-4	1.4107267429352567E-4
11	0.0	3.526816857338142E-5	0.0	2.1160901144028852E-4	1.4107267429352567E-4
12	0.0	3.526816857338142E-5	0.0	4.2321802288057704E-4	1.4107267429352567E-4
13	0.0	3.526816857338142E-5	0.0	4.5848619145395845E-4	1.4107267429352567E-4
14	0.0	3.526816857338142E-5	0.0	6.70095202894247E-4	1.763408428669071E-4
15	0.0	3.526816857338142E-5	0.0	0.0010580450572014427	1.763408428669071E-4
16	0.0	3.526816857338142E-5	0.0	0.0014812630800820196	2.8214534858705134E-4
17	0.0	3.526816857338142E-5	0.0	0.002045553777256122	2.8214534858705134E-4
18	0.0	3.526816857338142E-5	0.0	0.002609844474430225	3.1741351716043276E-4
19	0.0	3.526816857338142E-5	0.0	0.003421012351617998	3.1741351716043276E-4
20	0.0	3.526816857338142E-5	0.0	0.0059955886574748415	3.1741351716043276E-4
21	0.0	3.526816857338142E-5	0.0	0.008182215109024489	3.1741351716043276E-4
22	0.0	3.526816857338142E-5	0.0	0.014001462923632423	3.1741351716043276E-4
23	0.0	3.526816857338142E-5	0.0	0.02013812425540079	4.937543600273399E-4
24	0.0	3.526816857338142E-5	0.0	0.02955472526449363	5.290225286007213E-4
25	0.0	3.526816857338142E-5	0.0	0.03523290040480804	5.642906971741027E-4
26	0.0	3.526816857338142E-5	0.0	0.042603947636644754	5.642906971741027E-4
27	0.0	3.526816857338142E-5	0.0	0.06729166563801176	5.642906971741027E-4
28	0.0	3.526816857338142E-5	0.0	0.19524458122223953	5.642906971741027E-4
29	0.0	3.526816857338142E-5	0.0	0.4078411013825827	5.642906971741027E-4
30	0.0	3.526816857338142E-5	0.0	0.6690018896684722	5.642906971741027E-4
31	0.0	3.526816857338142E-5	0.0	1.261048635509826	5.642906971741027E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	135	2.2231688E-8	16.444443	5
TAGATCG	105	9.352694E-6	15.857142	5
CTTATAC	3920	0.0	15.385203	37
TCTATAC	310	0.0	14.919355	3
GTATTAG	415	0.0	14.710843	1
AACGCAG	11275	0.0	14.537473	5
GCACCGT	350	0.0	14.271428	6
ACCGTGC	340	0.0	14.147059	8
GTACCGT	185	4.9076334E-9	14.0	6
TCGTATA	80	0.0063016266	13.875	2
TAATACC	310	0.0	13.725807	4
TCGTTAC	270	0.0	13.703703	23
ATTGCGA	190	7.1377144E-9	13.631579	10
ACGCGCA	110	2.4583534E-4	13.454545	19
TAAGACT	510	0.0	13.058824	4
ACGCAGA	12620	0.0	13.032092	6
TAGACTA	200	1.4650141E-8	12.949999	5
CGACGCG	115	3.5800505E-4	12.869565	17
CGCAGAG	12780	0.0	12.868935	7
CGATTCA	130	7.004869E-5	12.807693	14
>>END_MODULE