FastQCFastQC Report
Thu 9 Feb 2017
SRR2727405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4923011
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT362210.7357489146378101No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138390.2811084517178613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT135630.2755021266456646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93520.18996504375066398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC55200.017.72916837
CGTATAC1251.6608465E-716.283
AACGCAG163800.014.2533585
TATACTG7600.014.1184225
GTACCGT3350.013.805976
TAGATCG2051.4279067E-913.5365865
GTATTAG7300.013.4315071
TTATACT10850.012.9585264
ACGCAGA184300.012.7883886
CGCAGAG187850.012.5861057
GTCTTAG8400.012.5535711
TATACAC20200.012.45544637
AGAGTAC190050.012.35280210
GAGTACT124400.012.32837711
TACCGTG4250.012.1882357
CAGAGTA195850.012.1097789
CTTACAC9800.012.0816343
TAATACT9050.012.0607734
TAGACAG8800.011.9829545
TCTTATA82650.011.930428537
Produced by FastQC (version 0.11.5)