##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727405.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4923011
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91666746225024	31.0	31.0	34.0	30.0	34.0
2	32.07773088461513	33.0	31.0	34.0	30.0	34.0
3	32.162095311182526	34.0	31.0	34.0	30.0	34.0
4	35.815662609732136	37.0	35.0	37.0	35.0	37.0
5	35.492390937172395	37.0	35.0	37.0	33.0	37.0
6	35.4907756655429	37.0	35.0	37.0	33.0	37.0
7	35.46520533876524	37.0	35.0	37.0	33.0	37.0
8	35.3974311656017	37.0	35.0	37.0	33.0	37.0
9	37.186291682062055	39.0	37.0	39.0	34.0	39.0
10	36.9468329443099	39.0	37.0	39.0	33.0	39.0
11	37.07271241116463	39.0	37.0	39.0	33.0	39.0
12	36.97985704277321	39.0	37.0	39.0	33.0	39.0
13	37.079696145306194	39.0	37.0	39.0	33.0	39.0
14	38.19492521954552	40.0	38.0	41.0	33.0	41.0
15	38.08347005521621	40.0	37.0	41.0	33.0	41.0
16	38.04942544308757	40.0	37.0	41.0	33.0	41.0
17	37.95843438091038	40.0	37.0	41.0	33.0	41.0
18	37.91755289598175	40.0	37.0	41.0	33.0	41.0
19	37.96431310025511	40.0	37.0	41.0	33.0	41.0
20	37.91607859498993	40.0	37.0	41.0	33.0	41.0
21	37.86528244604776	40.0	37.0	41.0	33.0	41.0
22	37.817722121685286	39.0	37.0	41.0	33.0	41.0
23	37.736989821879334	39.0	37.0	41.0	33.0	41.0
24	37.67818272191551	39.0	37.0	41.0	32.0	41.0
25	37.55807919177918	39.0	37.0	41.0	32.0	41.0
26	37.422568017824865	39.0	37.0	40.0	32.0	41.0
27	37.308794759954836	39.0	36.0	40.0	32.0	41.0
28	37.1978449773929	39.0	36.0	40.0	31.0	41.0
29	37.098555944725696	39.0	36.0	40.0	31.0	41.0
30	37.0114492939382	39.0	36.0	40.0	31.0	41.0
31	36.905734722104015	39.0	36.0	40.0	31.0	41.0
32	36.79784891807067	39.0	35.0	40.0	31.0	41.0
33	36.67659040371837	39.0	35.0	40.0	30.0	41.0
34	36.564309728334955	39.0	35.0	40.0	30.0	41.0
35	36.486708439205195	38.0	35.0	40.0	30.0	41.0
36	36.38182303472407	38.0	35.0	40.0	30.0	41.0
37	36.28568715365454	38.0	35.0	40.0	30.0	41.0
38	36.17967703098774	38.0	35.0	40.0	30.0	41.0
39	36.06043557489512	38.0	35.0	40.0	29.0	41.0
40	35.92212245717103	38.0	35.0	40.0	29.0	41.0
41	35.81026083427398	38.0	35.0	40.0	28.0	41.0
42	35.68056601945435	38.0	35.0	40.0	27.0	41.0
43	33.98725536059131	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	11.0
11	7.0
12	13.0
13	11.0
14	18.0
15	26.0
16	52.0
17	108.0
18	207.0
19	501.0
20	1070.0
21	2138.0
22	3959.0
23	6760.0
24	11384.0
25	17784.0
26	26812.0
27	38895.0
28	54898.0
29	74395.0
30	97270.0
31	123780.0
32	159213.0
33	206086.0
34	274175.0
35	376334.0
36	554265.0
37	873523.0
38	1329804.0
39	689506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28462804572242	18.575461237035626	12.754105160439414	25.38580555680253
2	16.966385002999182	21.561641848860383	36.521246854821165	24.950726293319274
3	18.480884970600307	23.230153253770915	30.968913130602388	27.320048645026386
4	13.717072742677194	15.55531767042568	35.65202271536667	35.07558687153045
5	14.349287458427373	37.23146261505408	34.74968875755102	13.669561168967528
6	34.56790976091664	36.595347847079765	15.000311801050211	13.836430590953382
7	29.3214863830286	31.300498820742025	21.54108532359566	17.83692947263372
8	27.30436718504184	36.08951919871802	19.66024451296168	16.94586910327846
9	26.58655444808066	13.783576758207527	19.10087139760606	40.52899739610576
10	14.701003105619712	27.8363993092845	34.95850405371834	22.504093531377446
11	36.430834706645996	21.216487227024274	22.474721263064414	19.877956803265317
12	20.81262869410611	25.46234408170122	31.677666371251252	22.047360852941424
13	31.12010109260369	19.32071652896977	25.316478065964105	24.242704312462436
14	21.536372760491496	19.628637839728572	27.65931256298229	31.175676836797646
15	24.79389138070177	28.35835629861481	22.76592516246663	24.08182715821679
16	24.05450647987583	26.427200751735064	25.036852446602293	24.48144032178681
17	23.598119118563822	26.913265072940117	26.53703190994292	22.95158389855314
18	23.530436962257447	25.988526127607674	27.16894193411309	23.31209497602179
19	24.37055289943492	26.19638672349097	27.02878380730817	22.40427656976594
20	23.769091720493822	26.16055499368171	26.853261144450013	23.217092141374458
21	23.491883321000095	26.438596216827463	26.852103316445973	23.21741714572647
22	23.944269066227967	25.933214449449736	26.487346869629175	23.635169614693122
23	23.34634230961499	25.793157886504826	26.754967640738563	24.10553216314162
24	23.389974143872518	26.02933448655711	26.78852434008374	23.79216702948663
25	23.751886802609214	25.62771035855902	26.766444356918967	23.853958481912795
26	23.367812909619744	25.94467085285814	27.1549870597486	23.53252917777352
27	23.85454755230082	26.358848273952667	26.630328471742192	23.156275702004322
28	23.673398251598464	26.309386674130934	26.809852750684488	23.207362323586114
29	23.436206012946144	26.50032673093763	26.86579412477445	23.197673131341777
30	23.44664677775451	26.47887644370488	26.734756432597855	23.33972034594276
31	23.446829592702514	25.728157016102543	26.93179032100477	23.893223070190174
32	22.924852290600203	26.30946792521894	26.96288917493786	23.802790609243
33	23.4521109134227	25.817309772413672	26.883872491855087	23.84670682230854
34	23.574251611462984	25.807194011957318	27.33861451863504	23.279939857944658
35	23.45469063546679	25.837053786798364	27.143997850096213	23.564257727638633
36	23.260622411771983	25.540934196571975	27.35630694304766	23.842136448608382
37	22.932022699116455	25.17079486517499	27.46132397429134	24.435858461417208
38	22.725868376081223	25.174776168487128	27.992238896073967	24.107116559357678
39	22.652214264806638	24.946663738919128	28.311454108065163	24.089667888209064
40	22.725218367377202	24.995333140632837	28.594837590247106	23.684610901742857
41	22.406856291810033	24.704921439338648	28.84381123665984	24.04441103219148
42	21.93015615849731	24.9828814113964	28.807695128042575	24.27926730206372
43	21.66680106950807	24.441830416385418	28.863088057288515	25.028280456817992
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	944.0
1	1120.0
2	1296.0
3	4640.5
4	7985.0
5	7985.0
6	10248.5
7	12512.0
8	11671.0
9	10830.0
10	13298.5
11	15767.0
12	15767.0
13	23619.5
14	31472.0
15	49213.5
16	66955.0
17	55624.5
18	44294.0
19	44294.0
20	47036.5
21	49779.0
22	43599.0
23	37419.0
24	43472.0
25	49525.0
26	49525.0
27	60098.5
28	70672.0
29	81204.5
30	91737.0
31	105788.5
32	119840.0
33	119840.0
34	138539.5
35	157239.0
36	179052.5
37	200866.0
38	225300.0
39	249734.0
40	249734.0
41	271846.5
42	293959.0
43	315804.0
44	337649.0
45	358311.0
46	378973.0
47	378973.0
48	395699.0
49	412425.0
50	417481.0
51	422537.0
52	417603.0
53	412669.0
54	412669.0
55	387051.0
56	361433.0
57	330937.0
58	300441.0
59	272606.0
60	244771.0
61	244771.0
62	214013.0
63	183255.0
64	154892.0
65	126529.0
66	106122.0
67	85715.0
68	85715.0
69	70182.5
70	54650.0
71	45883.0
72	37116.0
73	29770.5
74	22425.0
75	22425.0
76	17903.5
77	13382.0
78	10455.0
79	7528.0
80	5873.0
81	4218.0
82	4218.0
83	3333.5
84	2449.0
85	1848.5
86	1248.0
87	878.0
88	508.0
89	508.0
90	332.0
91	156.0
92	108.0
93	60.0
94	49.5
95	39.0
96	39.0
97	24.0
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4923011.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.273373315171895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.46555896997808	45.02876253276748
2	9.594847342571795	10.606791581361113
3	3.2295018203105643	5.35516379208159
4	1.6092432463542305	3.557932108426261
5	0.9007543173584511	2.4893864824303242
6	0.6054333868847254	2.0078607366448993
7	0.41552155575455996	1.6077094650205963
8	0.3033474299307051	1.341362859100625
9	0.2464736436383405	1.2261086745455372
>10	1.4699634166351512	15.386359677004268
>50	0.1097763758300624	4.124280543580627
>100	0.047112945134561574	4.426271925369284
>500	0.001766364727083614	0.6523301767773114
>1k	5.519880724236436E-4	0.7053561330388022
>5k	3.67992048280322E-5	0.19022119372980473
>10k+	1.1039761448409659E-4	1.2941021181214036
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36221	0.7357489146378101	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13839	0.2811084517178613	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13563	0.2755021266456646	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9352	0.18996504375066398	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.031277200071257E-5	0.0	0.0	2.031277200071257E-5	0.0
7	2.031277200071257E-5	0.0	0.0	2.031277200071257E-5	0.0
8	2.031277200071257E-5	0.0	0.0	2.031277200071257E-5	0.0
9	2.031277200071257E-5	0.0	0.0	2.031277200071257E-5	0.0
10	2.031277200071257E-5	0.0	2.031277200071257E-5	2.031277200071257E-5	0.0
11	4.062554400142514E-5	0.0	2.031277200071257E-5	1.2187663200427543E-4	0.0
12	4.062554400142514E-5	0.0	2.031277200071257E-5	1.8281494800641315E-4	2.031277200071257E-5
13	4.062554400142514E-5	0.0	2.031277200071257E-5	2.234404920078383E-4	2.031277200071257E-5
14	8.125108800285029E-5	0.0	2.031277200071257E-5	2.4375326400855087E-4	2.031277200071257E-5
15	8.125108800285029E-5	0.0	2.031277200071257E-5	3.8594266801353887E-4	2.031277200071257E-5
16	8.125108800285029E-5	0.0	2.031277200071257E-5	6.500087040228023E-4	2.031277200071257E-5
17	8.125108800285029E-5	0.0	2.031277200071257E-5	8.937619680313532E-4	2.031277200071257E-5
18	8.125108800285029E-5	2.031277200071257E-5	2.031277200071257E-5	0.0010562641440370537	8.125108800285029E-5
19	8.125108800285029E-5	2.031277200071257E-5	2.031277200071257E-5	0.0014015812680491675	8.125108800285029E-5
20	8.125108800285029E-5	2.031277200071257E-5	2.031277200071257E-5	0.002335968780081946	8.125108800285029E-5
21	8.125108800285029E-5	2.031277200071257E-5	2.031277200071257E-5	0.0037781755921325383	1.0156386000356286E-4
22	8.125108800285029E-5	2.031277200071257E-5	2.031277200071257E-5	0.006520399812228735	1.0156386000356286E-4
23	8.125108800285029E-5	2.031277200071257E-5	2.031277200071257E-5	0.009221998488323507	2.234404920078383E-4
24	8.125108800285029E-5	2.031277200071257E-5	2.031277200071257E-5	0.013629870012478135	2.234404920078383E-4
25	8.125108800285029E-5	2.031277200071257E-5	2.031277200071257E-5	0.017428358376611386	2.234404920078383E-4
26	8.125108800285029E-5	2.031277200071257E-5	2.031277200071257E-5	0.02147060000475319	2.4375326400855087E-4
27	8.125108800285029E-5	2.031277200071257E-5	2.031277200071257E-5	0.03562860208924985	2.4375326400855087E-4
28	1.0156386000356286E-4	2.031277200071257E-5	2.031277200071257E-5	0.11903284392417567	2.4375326400855087E-4
29	1.0156386000356286E-4	2.031277200071257E-5	2.031277200071257E-5	0.26136443733316866	2.4375326400855087E-4
30	1.0156386000356286E-4	4.062554400142514E-5	2.031277200071257E-5	0.4418637293315006	2.4375326400855087E-4
31	1.0156386000356286E-4	4.062554400142514E-5	2.031277200071257E-5	0.9767802671982655	2.640660360092634E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5520	0.0	17.729168	37
CGTATAC	125	1.6608465E-7	16.28	3
AACGCAG	16380	0.0	14.253358	5
TATACTG	760	0.0	14.118422	5
GTACCGT	335	0.0	13.80597	6
TAGATCG	205	1.4279067E-9	13.536586	5
GTATTAG	730	0.0	13.431507	1
TTATACT	1085	0.0	12.958526	4
ACGCAGA	18430	0.0	12.788388	6
CGCAGAG	18785	0.0	12.586105	7
GTCTTAG	840	0.0	12.553571	1
TATACAC	2020	0.0	12.455446	37
AGAGTAC	19005	0.0	12.352802	10
GAGTACT	12440	0.0	12.328377	11
TACCGTG	425	0.0	12.188235	7
CAGAGTA	19585	0.0	12.109778	9
CTTACAC	980	0.0	12.081634	3
TAATACT	905	0.0	12.060773	4
TAGACAG	880	0.0	11.982954	5
TCTTATA	8265	0.0	11.9304285	37
>>END_MODULE