FastQCFastQC Report
Thu 9 Feb 2017
SRR2727404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775117
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT360531.2991524321316903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT126330.45522405001302646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124980.45035939025273525No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90010.3243466852028221No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA45870.1652903282996717No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41900.1509846251527413No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36470.13141788256134787No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29450.1061216518078337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC2150.018.06976723
TCTATAC1950.018.0256423
CTTATAC50200.017.28386537
CGTTACG2150.016.34883724
AACGCAG142600.015.7366775
CATATAC2400.015.4166673
TATACAC17700.015.15536737
CGTATAC750.00410635214.83
ACGCAGA162350.013.9248546
CGCAGAG164100.013.7650817
GTATAGA3100.013.7258071
AGAGTAC168800.013.30509610
ACCGTGC2800.013.2142868
CAGAGTA172050.013.2043019
CCCTATA1551.890061E-613.1290322
CGGTGAT3250.013.09230814
GCAGAGT173000.013.0890188
CTATACG850.00940911613.0588234
GTTACGG2850.012.98245625
TTAGGAC3600.012.8472223
Produced by FastQC (version 0.11.5)