FastQCFastQC Report
Thu 9 Feb 2017
SRR2727403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4011490
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT372490.9285577179551738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT142270.35465624992209877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT139300.3472525171445024No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99720.24858593689626546No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58760.14647923838773125No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57930.14441018175291476No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA45900.11442132474467094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC52150.016.31831237
TAAGACT6950.016.237414
CGTTATA1302.5934605E-715.6538462
TATACCG2350.014.9574465
GTTACGG7300.014.19178125
CTATACT4850.014.1134024
TCTATAC4750.014.0210523
AACGCAG181050.013.9988965
TATACTG6600.013.7348485
CGTTACG4800.013.48958324
TCGTTAC4950.013.45454623
CCCTATA2900.013.3965512
GTATTAG4700.013.38297751
CTAAGAC5750.013.1913033
TACGGAT10150.013.12315327
CCTATAC5500.013.1181823
AGTCGGT5950.013.05882411
TTATACT7950.013.0314464
TACTTAC8850.012.96045331
GTGTAAG7800.012.8076931
Produced by FastQC (version 0.11.5)