FastQCFastQC Report
Thu 9 Feb 2017
SRR2727402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176726
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT197820.6227165956396618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT77630.24437109149482833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74220.23363676942865075No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53140.16727914211046216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC37750.018.18145837
TAGATCG1351.1550583E-917.8148165
TATACCG1659.767973E-1015.696975
AACGCAG92200.015.5303685
GGACCGT2055.456968E-1215.3414636
CTATACT4000.015.2625014
TCGTTAC4050.015.07407423
TAGACGG1001.09432796E-414.85
ATTACGG3650.014.69863125
TCTATAC3650.014.6986313
TAGACAG6450.014.0542645
ACGCAGA102850.014.0481286
CATTACG4250.013.92941224
ATACCGT2009.858923E-1013.8750016
TATACAC14400.013.87499937
CTAGACA4150.013.8192774
CTCGTTA4300.013.76744222
CGCAGAG105700.013.6518457
AGAGTAC106850.013.41834310
CGCTACG1805.1681127E-813.3611110
Produced by FastQC (version 0.11.5)