FastQCFastQC Report
Thu 9 Feb 2017
SRR2727401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541932
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT226540.8912118813563855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81890.3221565329048928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81840.3219598321276887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60890.23954220647916624No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32170.12655728005312494No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31730.12482631321372878No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA28410.1117653816073758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC656.906335E-519.9230773
TCGTTAC2550.016.68627423
AGTCGGT3200.016.187511
CTTATAC29350.015.88415737
GTATAAG2950.015.6779661
CGACGCG957.065058E-515.57894817
ATAACCG957.065058E-515.5789485
TTATACT4350.015.3103444
GTTATAC1954.1836756E-1115.1794863
ATAGCAC2700.015.0740753
GTTACGG3750.014.80000125
CGCGAAC750.004106224514.812
CTCGTTA3300.014.57575722
CGTTACG2450.014.34693824
AACGCAG107500.014.3181385
TATACTC2500.014.0599995
ACCGTAT800.006301389513.8750018
CGGTGAT3600.013.87514
GACTGCG2151.9826984E-1013.7674427
CGTAACG1356.5739932E-613.70370414
Produced by FastQC (version 0.11.5)