##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727401.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2541932
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30775606900578	33.0	31.0	34.0	30.0	34.0
2	32.4377796101548	34.0	31.0	34.0	31.0	34.0
3	32.530300967925186	34.0	31.0	34.0	31.0	34.0
4	36.067887732638006	37.0	35.0	37.0	35.0	37.0
5	35.87275702103754	37.0	35.0	37.0	35.0	37.0
6	35.89115562493411	37.0	35.0	37.0	35.0	37.0
7	35.85568142656845	37.0	35.0	37.0	35.0	37.0
8	35.80989459985555	37.0	35.0	37.0	35.0	37.0
9	37.64328117353257	39.0	37.0	39.0	35.0	39.0
10	37.4691840694401	39.0	37.0	39.0	35.0	39.0
11	37.58258285430137	39.0	37.0	39.0	35.0	39.0
12	37.505766479984516	39.0	37.0	39.0	35.0	39.0
13	37.58693938311489	39.0	37.0	39.0	35.0	39.0
14	38.80443300607569	40.0	38.0	41.0	35.0	41.0
15	38.730423158447984	40.0	38.0	41.0	35.0	41.0
16	38.70385714488035	40.0	38.0	41.0	35.0	41.0
17	38.636267610620585	40.0	38.0	41.0	34.0	41.0
18	38.61558649090534	40.0	38.0	41.0	34.0	41.0
19	38.66345362503797	40.0	38.0	41.0	34.0	41.0
20	38.62353752972149	40.0	38.0	41.0	34.0	41.0
21	38.575852540508556	40.0	38.0	41.0	34.0	41.0
22	38.53037256700809	40.0	38.0	41.0	34.0	41.0
23	38.45737533498142	40.0	38.0	41.0	34.0	41.0
24	38.41605991033592	40.0	38.0	41.0	34.0	41.0
25	38.30228031276997	40.0	38.0	41.0	34.0	41.0
26	38.185182373092594	40.0	38.0	41.0	34.0	41.0
27	38.092577220791114	40.0	38.0	41.0	33.0	41.0
28	37.98672663155427	40.0	37.0	41.0	33.0	41.0
29	37.89051359359731	40.0	37.0	41.0	33.0	41.0
30	37.81608162610172	40.0	37.0	41.0	33.0	41.0
31	37.70379577423786	40.0	37.0	41.0	33.0	41.0
32	37.5931425388248	40.0	37.0	41.0	33.0	41.0
33	37.47730348412153	40.0	36.0	41.0	32.0	41.0
34	37.36154822394934	39.0	36.0	41.0	32.0	41.0
35	37.2657604530727	39.0	36.0	41.0	32.0	41.0
36	37.15515481924772	39.0	36.0	41.0	31.0	41.0
37	37.05141876336582	39.0	36.0	41.0	31.0	41.0
38	36.936879900799866	39.0	36.0	41.0	31.0	41.0
39	36.812932840060235	39.0	36.0	41.0	31.0	41.0
40	36.67599015237229	39.0	35.0	41.0	30.0	41.0
41	36.55377169806273	39.0	35.0	40.0	30.0	41.0
42	36.40672567165447	39.0	35.0	40.0	30.0	41.0
43	34.724973366714764	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	9.0
12	5.0
13	2.0
14	4.0
15	8.0
16	11.0
17	26.0
18	60.0
19	149.0
20	288.0
21	638.0
22	1177.0
23	2202.0
24	3802.0
25	5997.0
26	9491.0
27	14151.0
28	20619.0
29	28857.0
30	38402.0
31	48854.0
32	62051.0
33	81333.0
34	109097.0
35	149531.0
36	220546.0
37	368312.0
38	687805.0
39	688496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.801591860049754	17.195739303805137	12.317166627588778	25.685502208556326
2	16.726057187997164	21.11181573700634	36.730683590277	25.431443484719495
3	18.733073898121585	23.03484121526461	30.344950218967305	27.887134667646503
4	13.484507059984296	14.896189197822759	34.87500845813342	36.744295284059525
5	14.626197711032397	37.08018153121327	34.33309781693609	13.960522940818244
6	35.32958395425212	35.50901440321771	14.896976000931575	14.264425641598594
7	29.722982361447908	30.514702989694452	21.41375143001465	18.34856321884299
8	27.834300838889476	35.909733226537924	19.30212924657308	16.95383668799952
9	26.84890862540776	13.645054234338291	18.643299663405628	40.862737476848324
10	15.052920377098994	27.704675026711968	34.3065038718581	22.935900724330942
11	36.75826103924102	20.994267352549166	21.831268499708095	20.416203108501723
12	21.041829600477115	25.14170323989784	31.56673742649292	22.24972973313212
13	31.717567582453032	18.919388874289318	24.861916054402712	24.501127488854934
14	21.71513636084679	19.190363864965704	27.607937584482983	31.486562189704525
15	25.044926457513416	27.999568831896372	22.642226463965205	24.313278246625007
16	24.106073647918198	25.999043247419678	24.891224470206126	25.003658634455995
17	23.85827000879646	26.455585751310423	26.50633455182908	23.17980968806404
18	23.821054221749442	25.614768609073728	26.95646461038297	23.60771255879386
19	24.688386628753246	25.92531979612358	26.87015230934581	22.51614126577737
20	24.006425034186595	25.79746429094091	26.70771680753065	23.48839386734185
21	23.4898887932486	26.21883669586755	26.93742397514961	23.353850535734235
22	23.922748523563968	25.656154452597473	26.414671989651968	24.006425034186595
23	23.30329843599278	25.58365054612004	26.843873085511333	24.269177932375847
24	23.402986389879825	25.646240733426385	26.905283068154457	24.04548980853933
25	23.80889811371823	25.532193622803444	26.634190057011754	24.024718206466577
26	23.484302491176003	25.88940223420611	27.05784419095397	23.568451083663923
27	23.98986282874601	26.153374677214025	26.595085942503577	23.26167655153639
28	23.65877608055605	26.34012239509161	26.61121540623431	23.38988611811803
29	23.462901446616197	26.44087253317555	26.869798247946836	23.226427772261413
30	23.624471465011652	26.29869721141242	26.681555604162504	23.395275719413423
31	23.38607012304027	25.72409490104377	26.965355485512593	23.924479490403364
32	22.980591140911717	26.16938612047844	26.95076028784405	23.899262450765796
33	23.403025730035264	25.622282578762924	26.888956903646516	24.085734787555293
34	23.573565303871227	25.755330984463786	27.1847948725615	23.486308839103483
35	23.507591863196968	25.825907223324624	26.95843161815501	23.7080692953234
36	23.271629610862917	25.737077152339243	27.17912989017802	23.812163346619815
37	23.03055313832156	25.349419260625382	27.260170610386115	24.35985699066694
38	22.94156570671442	25.406973908035308	27.586929941477585	24.06453044377269
39	22.68994607251492	25.27880368160911	27.957278164797483	24.073972081078487
40	23.03661152225945	25.21243683938044	28.067902681897078	23.683048956463036
41	22.57778728935314	25.065934100518817	28.21224171220945	24.144036897918593
42	22.193473310851747	25.352409112438885	28.20940922101771	24.244708355691653
43	22.05255687406272	24.821710415542196	28.03363740650812	25.092095303886964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	268.0
1	422.5
2	577.0
3	3065.0
4	5553.0
5	5553.0
6	6865.0
7	8177.0
8	7521.0
9	6865.0
10	8404.0
11	9943.0
12	9943.0
13	15203.5
14	20464.0
15	31286.5
16	42109.0
17	34128.0
18	26147.0
19	26147.0
20	26755.5
21	27364.0
22	22278.5
23	17193.0
24	19434.5
25	21676.0
26	21676.0
27	25849.0
28	30022.0
29	34683.0
30	39344.0
31	45401.5
32	51459.0
33	51459.0
34	58922.5
35	66386.0
36	76768.5
37	87151.0
38	98748.0
39	110345.0
40	110345.0
41	123743.5
42	137142.0
43	150814.5
44	164487.0
45	179389.0
46	194291.0
47	194291.0
48	207908.5
49	221526.0
50	225548.0
51	229570.0
52	228378.0
53	227186.0
54	227186.0
55	214858.0
56	202530.0
57	185201.5
58	167873.0
59	152856.5
60	137840.0
61	137840.0
62	119882.5
63	101925.0
64	86307.0
65	70689.0
66	58239.0
67	45789.0
68	45789.0
69	36812.5
70	27836.0
71	22904.0
72	17972.0
73	14351.5
74	10731.0
75	10731.0
76	8445.0
77	6159.0
78	4838.5
79	3518.0
80	2711.5
81	1905.0
82	1905.0
83	1467.5
84	1030.0
85	787.0
86	544.0
87	379.5
88	215.0
89	215.0
90	148.5
91	82.0
92	57.0
93	32.0
94	22.5
95	13.0
96	13.0
97	8.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2541932.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.1407333196458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.70825404768819	47.50577808600509
2	9.565687279560647	11.123121462801272
3	3.1940598236589643	5.571149412430521
4	1.5273413969117264	3.5520299538359987
5	0.9076383233830565	2.638537885525236
6	0.6039641546220536	2.106895130910368
7	0.42235310849732505	1.718914361350648
8	0.31999876136478084	1.4883970117701357
9	0.24903569316178173	1.3031206040873058
>10	1.4007109711888726	14.604949968733328
>50	0.07435715446393518	2.9132919098692813
>100	0.024296909784465723	2.46278959821039
>500	0.0014220555882499136	0.5284785611586955
>1k	6.094523949583342E-4	0.7062785449267903
>5k	2.031507983194447E-4	0.8843541265480552
>10k+	6.771693277314823E-5	0.8919133818368641
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22654	0.8912118813563855	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8189	0.3221565329048928	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8184	0.3219598321276887	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6089	0.23954220647916624	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3217	0.12655728005312494	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3173	0.12482631321372878	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2841	0.1117653816073758	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1802046632246653E-4
2	0.0	0.0	0.0	0.0	1.1802046632246653E-4
3	0.0	0.0	0.0	0.0	1.1802046632246653E-4
4	0.0	0.0	0.0	0.0	1.967007772041109E-4
5	0.0	0.0	0.0	0.0	1.967007772041109E-4
6	0.0	0.0	0.0	0.0	2.3604093264493307E-4
7	0.0	0.0	0.0	0.0	2.3604093264493307E-4
8	0.0	0.0	0.0	0.0	2.3604093264493307E-4
9	0.0	0.0	0.0	0.0	3.540613989673996E-4
10	0.0	0.0	0.0	7.868031088164436E-5	3.540613989673996E-4
11	0.0	0.0	0.0	2.3604093264493307E-4	3.540613989673996E-4
12	0.0	0.0	0.0	3.934015544082218E-4	3.540613989673996E-4
13	0.0	0.0	0.0	5.114220207306883E-4	3.540613989673996E-4
14	3.934015544082218E-5	0.0	0.0	6.68782642493977E-4	3.540613989673996E-4
15	3.934015544082218E-5	0.0	0.0	9.0482357513891E-4	3.934015544082218E-4
16	3.934015544082218E-5	0.0	0.0	0.0014162455958695985	4.7208186528986613E-4
17	3.934015544082218E-5	0.0	0.0	0.002242388860126864	4.7208186528986613E-4
18	3.934015544082218E-5	0.0	0.0	0.0027931510362983747	5.507621761715105E-4
19	7.868031088164436E-5	0.0	0.0	0.0038946753886413958	5.507621761715105E-4
20	7.868031088164436E-5	0.0	0.0	0.0057830028498008605	5.507621761715105E-4
21	7.868031088164436E-5	0.0	0.0	0.008969555440507456	5.901023316123327E-4
22	7.868031088164436E-5	0.0	0.0	0.01337565284987954	5.901023316123327E-4
23	7.868031088164436E-5	0.0	0.0	0.019434036787766156	7.474629533756213E-4
24	7.868031088164436E-5	0.0	0.0	0.03088202202104541	7.474629533756213E-4
25	7.868031088164436E-5	0.0	0.0	0.04437569533724742	7.474629533756213E-4
26	7.868031088164436E-5	0.0	0.0	0.055705660104204205	7.474629533756213E-4
27	7.868031088164436E-5	0.0	0.0	0.07600518031166845	7.474629533756213E-4
28	7.868031088164436E-5	0.0	0.0	0.17337206502770333	7.474629533756213E-4
29	7.868031088164436E-5	0.0	0.0	0.3443050404180757	7.474629533756213E-4
30	7.868031088164436E-5	0.0	0.0	0.5556796956016132	7.474629533756213E-4
31	7.868031088164436E-5	0.0	0.0	1.0490052448295233	7.474629533756213E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	65	6.906335E-5	19.923077	3
TCGTTAC	255	0.0	16.686274	23
AGTCGGT	320	0.0	16.1875	11
CTTATAC	2935	0.0	15.884157	37
GTATAAG	295	0.0	15.677966	1
CGACGCG	95	7.065058E-5	15.578948	17
ATAACCG	95	7.065058E-5	15.578948	5
TTATACT	435	0.0	15.310344	4
GTTATAC	195	4.1836756E-11	15.179486	3
ATAGCAC	270	0.0	15.074075	3
GTTACGG	375	0.0	14.800001	25
CGCGAAC	75	0.0041062245	14.8	12
CTCGTTA	330	0.0	14.575757	22
CGTTACG	245	0.0	14.346938	24
AACGCAG	10750	0.0	14.318138	5
TATACTC	250	0.0	14.059999	5
ACCGTAT	80	0.0063013895	13.875001	8
CGGTGAT	360	0.0	13.875	14
GACTGCG	215	1.9826984E-10	13.767442	7
CGTAACG	135	6.5739932E-6	13.703704	14
>>END_MODULE