FastQCFastQC Report
Thu 9 Feb 2017
SRR2727400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292360
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78330.6061004673620353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28580.22114581076480236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28110.2175090532049893No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.15011297161781548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG1059.822543E-1021.1428595
CCGTATA450.003826062420.5555552
CGCGACA555.14357E-420.18181812
TGTTCGG701.2193462E-418.510
TATACCG609.2376635E-418.55
CGATACT801.6169246E-518.54
GATAATC1002.875604E-718.4999987
TAAGACT2400.017.7291664
GGCCTAA1554.0017767E-1016.7096791
CTATACT2050.016.2439024
TACGATA803.3829737E-416.18752
TGAACCG1151.2424098E-616.0869565
GCGTTCC1059.344814E-615.8571449
TAATCGA700.002592727515.85714220
TAGGGTG2150.015.4883725
CTTATAC12450.015.453814537
AACGCAG33400.015.4535925
CGCGCCA1201.9358558E-615.41666610
CGACCGT855.364792E-415.23529414
TATACTA1701.4861143E-915.2352945
Produced by FastQC (version 0.11.5)