##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727400.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1292360
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36331440155994	33.0	31.0	34.0	31.0	34.0
2	32.508168776501904	34.0	31.0	34.0	31.0	34.0
3	32.60087127425795	34.0	31.0	34.0	31.0	34.0
4	36.12832028227429	37.0	35.0	37.0	35.0	37.0
5	35.93377696617042	37.0	35.0	37.0	35.0	37.0
6	35.94436689467331	37.0	35.0	37.0	35.0	37.0
7	35.913403386053425	37.0	35.0	37.0	35.0	37.0
8	35.868917329536664	37.0	35.0	37.0	35.0	37.0
9	37.709679965334736	39.0	37.0	39.0	35.0	39.0
10	37.52651351016745	39.0	37.0	39.0	35.0	39.0
11	37.63334132904144	39.0	37.0	39.0	35.0	39.0
12	37.5603887461698	39.0	37.0	39.0	35.0	39.0
13	37.644843542047106	39.0	37.0	39.0	35.0	39.0
14	38.89693274319849	40.0	38.0	41.0	36.0	41.0
15	38.81894131666099	40.0	38.0	41.0	35.0	41.0
16	38.79069531709431	40.0	38.0	41.0	35.0	41.0
17	38.71521789594231	40.0	38.0	41.0	35.0	41.0
18	38.68373208703457	40.0	38.0	41.0	35.0	41.0
19	38.72879073942245	40.0	38.0	41.0	35.0	41.0
20	38.6999450617475	40.0	38.0	41.0	34.0	41.0
21	38.656741155715125	40.0	38.0	41.0	34.0	41.0
22	38.61452072178031	40.0	38.0	41.0	34.0	41.0
23	38.553956327958154	40.0	38.0	41.0	34.0	41.0
24	38.50964437153734	40.0	38.0	41.0	34.0	41.0
25	38.40426583923984	40.0	38.0	41.0	34.0	41.0
26	38.292107462317	40.0	38.0	41.0	34.0	41.0
27	38.20345724101644	40.0	38.0	41.0	34.0	41.0
28	38.11681729549042	40.0	38.0	41.0	34.0	41.0
29	38.04106905196694	40.0	38.0	41.0	33.0	41.0
30	37.9803413909437	40.0	37.0	41.0	33.0	41.0
31	37.887864062645086	40.0	37.0	41.0	33.0	41.0
32	37.793992386022474	40.0	37.0	41.0	33.0	41.0
33	37.70300767587979	40.0	37.0	41.0	33.0	41.0
34	37.60520597975796	40.0	37.0	41.0	33.0	41.0
35	37.537776625708005	40.0	37.0	41.0	33.0	41.0
36	37.44728790739423	40.0	37.0	41.0	32.0	41.0
37	37.36695967068	40.0	37.0	41.0	32.0	41.0
38	37.275700269274815	40.0	36.0	41.0	32.0	41.0
39	37.17332554396608	39.0	36.0	41.0	31.0	41.0
40	37.05286762202482	39.0	36.0	41.0	31.0	41.0
41	36.948376613327554	39.0	36.0	41.0	31.0	41.0
42	36.832139651490294	39.0	36.0	41.0	31.0	41.0
43	35.16577501624934	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	5.0
12	2.0
13	0.0
14	0.0
15	0.0
16	5.0
17	15.0
18	20.0
19	56.0
20	135.0
21	236.0
22	544.0
23	912.0
24	1548.0
25	2602.0
26	3991.0
27	6019.0
28	8814.0
29	12577.0
30	16880.0
31	21914.0
32	29267.0
33	38475.0
34	52904.0
35	73548.0
36	108581.0
37	183395.0
38	357726.0
39	372186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.977993747872105	17.820421554365655	12.169751462440805	26.03183323532143
2	16.76932124175926	20.585363211489057	37.22198149122535	25.423334055526325
3	18.234006004518864	22.745132935095487	30.535841406419262	28.485019653966386
4	13.223792132223219	15.030022594323563	35.938902472995146	35.807282800458076
5	14.184902039679345	37.2422544801758	34.98258999040515	13.590253489739702
6	34.87054690643474	36.54136618279736	14.945603392243648	13.642483518524251
7	29.86404716951933	30.656937695378993	21.39388405707388	18.085131078027793
8	27.24720666068278	36.42421616267913	19.197746757869325	17.130830418768763
9	26.76019065894952	13.519452784053978	18.720093472407072	41.000263084589434
10	14.74411154786592	27.488702838218455	34.9084620384413	22.858723575474325
11	36.806462595561605	21.146816676467857	22.143210870036214	19.90350985793432
12	20.830418768764122	25.06453310223158	31.834009099631682	22.271039029372623
13	31.37330155684175	19.08763811940945	24.92571729239531	24.613343031353494
14	21.55962734841685	19.209585564393823	27.284657525766814	31.946129561422516
15	24.79997833421028	28.174347704973847	22.429973072518493	24.59570088829738
16	24.41262496517998	26.248258999040512	24.584558482156673	24.75455755362283
17	23.892104367204183	26.65720077996843	26.07245659104274	23.37823826178464
18	23.907038286545546	25.953062614132282	26.491225355164193	23.648673744157975
19	24.741867591073692	25.913986814819403	26.461202760840635	22.882942833266274
20	24.083459717106688	25.89882076201678	26.320839394595936	23.696880126280604
21	23.86393884057074	26.001501129716175	26.477684236590427	23.65687579312266
22	24.194496889411617	25.527097712711626	26.131728001485655	24.146677396391098
23	23.627781732644156	25.577470673806058	26.30644711999752	24.48830047355226
24	23.685428208858212	25.763873843201583	26.45114364418583	24.099554303754374
25	24.19813364697143	25.480516264817854	26.202064440248847	24.11928564796187
26	23.71320684638955	25.851156024637095	26.55274072239933	23.88289640657402
27	24.013742300906866	26.22992045560061	26.11656194868303	23.639775294809496
28	23.88042031632053	26.004364109071776	26.42313287319323	23.692082701414467
29	23.763270296202297	26.267139187223375	26.258627626977006	23.710962889597326
30	23.801185428208857	26.256770559286885	26.215528181002195	23.726515831502056
31	23.852409545327927	25.505122411711906	26.510879321551272	24.131588721408896
32	23.36864341205237	26.171577579002754	26.473351078646818	23.98642793029806
33	23.64635240954533	25.667074189854223	26.410133399362408	24.276440001238043
34	23.831208022532422	25.64718809000588	26.86534711690241	23.656256770559285
35	23.70515955306571	25.87119688012628	26.53339626729394	23.890247299514066
36	23.548005199789532	25.535609272958	26.806694729022873	24.109690798229597
37	23.551719335169764	25.112507350892937	26.79106440929772	24.544708904639574
38	23.24832090129685	25.165898046983813	27.272741341421895	24.313039710297442
39	23.12057011978087	25.08163360054474	27.50758302640131	24.290213253273084
40	23.23617258349067	24.973536785415828	27.78211953325699	24.008171097836517
41	22.784363490049213	24.84764307158996	28.053947816397905	24.314045621962922
42	22.550063449812747	25.00866631588721	27.87094927110093	24.57032096319911
43	22.388266427311276	24.431737286824102	27.94027979819864	25.239716487665977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	114.0
1	146.5
2	179.0
3	912.0
4	1645.0
5	1645.0
6	2047.5
7	2450.0
8	2249.5
9	2049.0
10	2611.5
11	3174.0
12	3174.0
13	4974.0
14	6774.0
15	10543.5
16	14313.0
17	11854.5
18	9396.0
19	9396.0
20	10160.0
21	10924.0
22	9774.5
23	8625.0
24	10301.0
25	11977.0
26	11977.0
27	14906.5
28	17836.0
29	20465.0
30	23094.0
31	26820.5
32	30547.0
33	30547.0
34	35066.0
35	39585.0
36	45319.5
37	51054.0
38	57965.0
39	64876.0
40	64876.0
41	70615.5
42	76355.0
43	82196.0
44	88037.0
45	93759.5
46	99482.0
47	99482.0
48	104386.0
49	109290.0
50	111025.0
51	112760.0
52	110919.0
53	109078.0
54	109078.0
55	104047.5
56	99017.0
57	91297.0
58	83577.0
59	75642.5
60	67708.0
61	67708.0
62	59348.5
63	50989.0
64	42999.5
65	35010.0
66	29378.5
67	23747.0
68	23747.0
69	19309.5
70	14872.0
71	12445.5
72	10019.0
73	7982.5
74	5946.0
75	5946.0
76	4776.0
77	3606.0
78	2851.0
79	2096.0
80	1572.5
81	1049.0
82	1049.0
83	821.0
84	593.0
85	460.0
86	327.0
87	225.0
88	123.0
89	123.0
90	85.0
91	47.0
92	29.5
93	12.0
94	9.0
95	6.0
96	6.0
97	3.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1292360.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.3078262748916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.0563204892368	54.69787081603342
2	7.916033356293509	10.1812579572554
3	2.477767022800167	4.780194337556655
4	1.2592727887746251	3.2392438293286743
5	0.7406020001032935	2.3813252380739924
6	0.5050749197889065	1.9488162118553884
7	0.34449247322843246	1.5507493484967245
8	0.2713709024589258	1.3961018281111321
9	0.19399966680286684	1.1228127183131054
>10	1.1608349917049614	13.26093155802015
>50	0.058431616280294495	2.4917687064801295
>100	0.014714303296013299	1.4502319903324428
>500	6.030384618140262E-4	0.3010110790671334
>1k	3.618230770884158E-4	0.5901554497866061
>5k	1.2060769236280525E-4	0.6075289312890637
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7833	0.6061004673620353	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2858	0.22114581076480236	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2811	0.2175090532049893	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1940	0.15011297161781548	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.737782042155437E-5
7	0.0	0.0	0.0	0.0	7.737782042155437E-5
8	0.0	0.0	0.0	0.0	7.737782042155437E-5
9	0.0	0.0	0.0	7.737782042155437E-5	7.737782042155437E-5
10	0.0	0.0	0.0	1.5475564084310874E-4	7.737782042155437E-5
11	0.0	0.0	0.0	3.8688910210777185E-4	7.737782042155437E-5
12	0.0	7.737782042155437E-5	0.0	4.6426692252932617E-4	7.737782042155437E-5
13	0.0	7.737782042155437E-5	0.0	4.6426692252932617E-4	7.737782042155437E-5
14	0.0	7.737782042155437E-5	0.0	4.6426692252932617E-4	7.737782042155437E-5
15	0.0	7.737782042155437E-5	0.0	6.19022563372435E-4	7.737782042155437E-5
16	7.737782042155437E-5	7.737782042155437E-5	0.0	9.285338450586523E-4	7.737782042155437E-5
17	7.737782042155437E-5	7.737782042155437E-5	0.0	0.0010832894859017612	7.737782042155437E-5
18	7.737782042155437E-5	7.737782042155437E-5	0.0	0.0011606673063233154	7.737782042155437E-5
19	7.737782042155437E-5	7.737782042155437E-5	0.0	0.001470178588009533	7.737782042155437E-5
20	7.737782042155437E-5	7.737782042155437E-5	0.0	0.002321334612646631	7.737782042155437E-5
21	7.737782042155437E-5	7.737782042155437E-5	0.0	0.003095112816862175	7.737782042155437E-5
22	7.737782042155437E-5	7.737782042155437E-5	0.0	0.005184313968244142	7.737782042155437E-5
23	7.737782042155437E-5	7.737782042155437E-5	0.0	0.007505648580890773	7.737782042155437E-5
24	7.737782042155437E-5	7.737782042155437E-5	0.0	0.011606673063233154	7.737782042155437E-5
25	7.737782042155437E-5	7.737782042155437E-5	0.0	0.014779163700516884	7.737782042155437E-5
26	7.737782042155437E-5	7.737782042155437E-5	0.0	0.018183787799065276	7.737782042155437E-5
27	7.737782042155437E-5	7.737782042155437E-5	0.0	0.03009997214398465	7.737782042155437E-5
28	7.737782042155437E-5	7.737782042155437E-5	0.0	0.1036089015444613	7.737782042155437E-5
29	7.737782042155437E-5	7.737782042155437E-5	0.0	0.23708564177164257	7.737782042155437E-5
30	7.737782042155437E-5	7.737782042155437E-5	0.0	0.4045312451638862	7.737782042155437E-5
31	7.737782042155437E-5	7.737782042155437E-5	0.0	0.8396267293942864	7.737782042155437E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	105	9.822543E-10	21.142859	5
CCGTATA	45	0.0038260624	20.555555	2
CGCGACA	55	5.14357E-4	20.181818	12
TGTTCGG	70	1.2193462E-4	18.5	10
TATACCG	60	9.2376635E-4	18.5	5
CGATACT	80	1.6169246E-5	18.5	4
GATAATC	100	2.875604E-7	18.499998	7
TAAGACT	240	0.0	17.729166	4
GGCCTAA	155	4.0017767E-10	16.709679	1
CTATACT	205	0.0	16.243902	4
TACGATA	80	3.3829737E-4	16.1875	2
TGAACCG	115	1.2424098E-6	16.086956	5
GCGTTCC	105	9.344814E-6	15.857144	9
TAATCGA	70	0.0025927275	15.857142	20
TAGGGTG	215	0.0	15.488372	5
CTTATAC	1245	0.0	15.4538145	37
AACGCAG	3340	0.0	15.453592	5
CGCGCCA	120	1.9358558E-6	15.416666	10
CGACCGT	85	5.364792E-4	15.235294	14
TATACTA	170	1.4861143E-9	15.235294	5
>>END_MODULE