FastQCFastQC Report
Thu 9 Feb 2017
SRR2727399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245932
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT136610.6082552811037912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52340.23304356498772003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47680.21229494036328794No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31380.13971927912332163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA603.7283247E-521.5833342
CGTATAC953.609488E-617.5263163
AACGCAG61950.016.5439875
AACGACG1352.2224413E-816.44444315
CGTTACG2150.016.34883724
CTAATAC2350.015.7446793
CGCGCAA1302.591969E-715.65384820
TAATACT4050.015.5308644
CCTATAC3000.015.4166673
TATCGTA855.3669815E-415.2352947
ATACCGT855.3669815E-415.2352946
TCGTTAC2600.014.94230923
ACGCAGA69300.014.8427126
TATACCG750.00410602714.85
TTAGACT2500.014.7999994
TATACTA3550.014.5915495
ACTGCGT1651.6472768E-814.5757598
CTCGTTA3050.014.55737722
CTTATAC21800.014.51146937
CGCAGAG71050.014.5031687
Produced by FastQC (version 0.11.5)