##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727399.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2245932
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.697898244470448	31.0	31.0	34.0	30.0	34.0
2	31.870053946424022	31.0	31.0	34.0	30.0	34.0
3	31.942082841332684	33.0	31.0	34.0	30.0	34.0
4	35.66741557625075	37.0	35.0	37.0	33.0	37.0
5	35.18489384362483	37.0	35.0	37.0	32.0	37.0
6	35.17677160305833	37.0	35.0	37.0	32.0	37.0
7	35.15711027760413	37.0	35.0	37.0	32.0	37.0
8	35.07557797831813	37.0	35.0	37.0	32.0	37.0
9	36.840544148264506	38.0	37.0	39.0	33.0	39.0
10	36.5262100544451	38.0	35.0	39.0	32.0	39.0
11	36.67533789981175	38.0	35.0	39.0	32.0	39.0
12	36.55082878733639	38.0	35.0	39.0	32.0	39.0
13	36.6807975486346	38.0	35.0	39.0	32.0	39.0
14	37.7190115283989	39.0	37.0	40.0	33.0	41.0
15	37.596650299296684	39.0	37.0	40.0	32.0	41.0
16	37.55426878462928	39.0	37.0	40.0	32.0	41.0
17	37.44294306328063	39.0	36.0	40.0	32.0	41.0
18	37.38557667818972	39.0	36.0	40.0	32.0	41.0
19	37.43056201167266	39.0	36.0	40.0	32.0	41.0
20	37.369524990070936	39.0	36.0	40.0	32.0	41.0
21	37.308497763957234	39.0	36.0	40.0	32.0	41.0
22	37.2587420278085	39.0	36.0	40.0	32.0	41.0
23	37.181440043598826	39.0	36.0	40.0	32.0	41.0
24	37.1111222423475	39.0	36.0	40.0	31.0	41.0
25	36.981608525992776	39.0	36.0	40.0	31.0	41.0
26	36.825929725387944	39.0	36.0	40.0	31.0	41.0
27	36.70211475681365	39.0	36.0	40.0	30.0	41.0
28	36.57681666230322	38.0	35.0	40.0	30.0	41.0
29	36.475450280774304	38.0	35.0	40.0	30.0	41.0
30	36.38918809652296	38.0	35.0	40.0	30.0	41.0
31	36.2773757175195	38.0	35.0	40.0	30.0	41.0
32	36.15708534363463	38.0	35.0	40.0	30.0	41.0
33	36.048745465134296	38.0	35.0	40.0	29.0	41.0
34	35.930812241866626	38.0	35.0	40.0	29.0	41.0
35	35.860419193457325	38.0	35.0	40.0	29.0	41.0
36	35.760220701250084	38.0	34.0	40.0	29.0	41.0
37	35.65697091452457	38.0	34.0	40.0	28.0	41.0
38	35.53768190666503	38.0	34.0	40.0	27.0	41.0
39	35.40380162890061	38.0	34.0	40.0	27.0	41.0
40	35.263361936158354	38.0	34.0	40.0	27.0	41.0
41	35.143212261101404	38.0	34.0	40.0	26.0	41.0
42	35.028739961851024	38.0	33.0	40.0	26.0	41.0
43	33.30794298313573	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	0.0
12	2.0
13	2.0
14	9.0
15	12.0
16	28.0
17	81.0
18	135.0
19	348.0
20	668.0
21	1314.0
22	2379.0
23	4120.0
24	6747.0
25	10432.0
26	15400.0
27	22081.0
28	30897.0
29	41167.0
30	53743.0
31	69209.0
32	88717.0
33	114982.0
34	152860.0
35	209806.0
36	300736.0
37	430658.0
38	508352.0
39	181043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76344965030108	18.532261885043717	12.914015206159402	25.790273258495805
2	16.99009587111275	20.94128406380959	37.31137006819441	24.757249996883253
3	18.619575303259403	22.99067825740049	30.802579953444713	27.587166485895388
4	13.831273609352376	15.693618506704565	35.71550696993497	34.75960091400808
5	14.159288883189697	37.47829408904633	34.907735407839596	13.45468161992438
6	34.590361595987765	36.9154097274539	14.906283894614797	13.58794478194353
7	29.339490242803436	31.173873474352742	21.347752291698946	18.13888399114488
8	26.829440962593704	37.29814615936725	19.0478607544663	16.824552123572754
9	26.736784550912496	13.671607154624448	18.590545038763416	41.00106325569964
10	14.747107214287878	28.03210426673648	34.82621023254489	22.394578286430754
11	37.097783904410285	21.126018062879908	22.00796818425491	19.768229848454897
12	20.820487886543315	25.212116840581107	31.731548417316287	22.235846855559295
13	31.281846467301772	19.443108696078067	24.84184739342064	24.43319744319953
14	21.448066994014066	19.410204761319577	27.376118244007387	31.765610000658967
15	24.77261110309662	28.460389717943375	22.431311366506197	24.335687812453806
16	24.150731188655755	26.6234240395524	24.70364196244588	24.522202809345963
17	23.77859169378236	26.860074125129348	26.065081222405666	23.296252958682633
18	23.78714048332719	26.289932197412924	26.52275313767291	23.400174181586976
19	24.87768997458516	26.060539677959977	26.408635702238538	22.65313464521633
20	24.13180808679871	25.96334172183307	26.324260930428885	23.580589260939334
21	23.789055055985667	26.09455673635711	26.63264070328042	23.483747504376804
22	24.149529015126014	25.485188331614673	26.261480757209032	24.103801896050282
23	23.546126953086734	25.60718668241069	26.622578065587028	24.22410829891555
24	23.656014518694242	25.667295358897775	26.60797388344794	24.06871623896004
25	24.078333627197974	25.534343871497445	26.459171515433237	23.928150985871344
26	23.75935691730649	25.83671277670027	26.699561696436046	23.704368609557193
27	24.105983618382034	26.20061515664766	26.12211767765008	23.57128354732022
28	23.77868074367345	26.33223089568161	26.21432883987583	23.674759520769108
29	23.71585604550806	26.47092610105738	26.34883870037027	23.464379153064296
30	24.069072438524408	26.205290275929993	26.128395694972067	23.597241590573535
31	23.892798179107828	25.60469328546011	26.575381623308274	23.927126912123786
32	23.402444953809823	26.173054215354696	26.5812143911748	23.843286439660684
33	23.679968939397988	25.54195763718581	26.493099523939286	24.28497389947692
34	23.81167372832303	25.697171597359137	26.98572352146013	23.5054311528577
35	23.810293455011106	25.761599193564187	26.580457467100516	23.84764988432419
36	23.687938904650718	25.522589285873305	26.8122988585585	23.97717295091748
37	23.501824632268477	25.290080020232136	26.96199172548412	24.24610362201527
38	23.481699356881684	25.260515456389598	27.20518697805633	24.05259820867239
39	23.15617747999494	25.20027320506587	27.547272134686178	24.096277180253008
40	23.520480584452244	25.02088219946107	27.676928776116107	23.78170843997058
41	22.954524001617145	25.058728403175163	27.809212389333247	24.17753520587444
42	22.725843881292935	25.262830753557992	27.63979497153075	24.37153039361833
43	22.48714564822087	24.77764242194332	27.5897934576826	25.145418472153207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	355.0
1	396.0
2	437.0
3	1333.5
4	2230.0
5	2230.0
6	2916.5
7	3603.0
8	3597.0
9	3591.0
10	4514.0
11	5437.0
12	5437.0
13	8233.0
14	11029.0
15	17862.5
16	24696.0
17	20282.5
18	15869.0
19	15869.0
20	17407.5
21	18946.0
22	17365.0
23	15784.0
24	18842.5
25	21901.0
26	21901.0
27	26401.0
28	30901.0
29	35809.5
30	40718.0
31	46783.5
32	52849.0
33	52849.0
34	61712.5
35	70576.0
36	80539.0
37	90502.0
38	101927.5
39	113353.0
40	113353.0
41	124246.0
42	135139.0
43	146472.5
44	157806.0
45	168239.5
46	178673.0
47	178673.0
48	187119.0
49	195565.0
50	197750.5
51	199936.0
52	194731.0
53	189526.0
54	189526.0
55	178698.5
56	167871.0
57	155234.5
58	142598.0
59	128867.5
60	115137.0
61	115137.0
62	100127.0
63	85117.0
64	71682.5
65	58248.0
66	48146.0
67	38044.0
68	38044.0
69	30754.0
70	23464.0
71	19339.0
72	15214.0
73	12164.0
74	9114.0
75	9114.0
76	7253.5
77	5393.0
78	4204.5
79	3016.0
80	2329.0
81	1642.0
82	1642.0
83	1268.5
84	895.0
85	682.0
86	469.0
87	333.5
88	198.0
89	198.0
90	130.0
91	62.0
92	37.0
93	12.0
94	12.0
95	12.0
96	12.0
97	7.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2245932.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.232692052014535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.12833045497067	51.732997900022916
2	8.913958266026876	11.094792394683202
3	2.949670058150638	5.5069772515180935
4	1.408108813345454	3.505216086266211
5	0.8286024694939257	2.5783081158777126
6	0.5633029186729234	2.103351424186382
7	0.38252590138379317	1.666393163591582
8	0.2997906207614769	1.4925421905545015
9	0.22252282581098548	1.246337504391522
>10	1.2281069485557647	13.577383138109852
>50	0.05740583845607486	2.38060586688291
>100	0.016957496406020026	1.667124187211828
>500	2.1521638959703723E-4	0.09629923119505453
>1k	3.586939826617029E-4	0.5081045666346098
>5k	7.173879653234058E-5	0.2336718479716675
>10k+	7.173879653234058E-5	0.6098951309019774
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13661	0.6082552811037912	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5234	0.23304356498772003	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4768	0.21229494036328794	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3138	0.13971927912332163	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.45249455459916E-5
2	0.0	0.0	0.0	0.0	4.45249455459916E-5
3	0.0	0.0	0.0	0.0	4.45249455459916E-5
4	0.0	0.0	0.0	0.0	4.45249455459916E-5
5	0.0	0.0	0.0	0.0	4.45249455459916E-5
6	8.90498910919832E-5	0.0	0.0	0.0	1.335748366379748E-4
7	8.90498910919832E-5	0.0	0.0	0.0	1.335748366379748E-4
8	8.90498910919832E-5	0.0	0.0	0.0	1.335748366379748E-4
9	8.90498910919832E-5	0.0	0.0	0.0	1.780997821839664E-4
10	8.90498910919832E-5	0.0	0.0	0.0	1.780997821839664E-4
11	8.90498910919832E-5	0.0	0.0	8.90498910919832E-5	1.780997821839664E-4
12	8.90498910919832E-5	0.0	0.0	8.90498910919832E-5	1.780997821839664E-4
13	8.90498910919832E-5	0.0	0.0	1.780997821839664E-4	1.780997821839664E-4
14	8.90498910919832E-5	0.0	0.0	4.007245099139244E-4	2.671496732759496E-4
15	8.90498910919832E-5	0.0	0.0	5.788242920978907E-4	3.561995643679328E-4
16	8.90498910919832E-5	0.0	0.0	7.123991287358656E-4	4.007245099139244E-4
17	8.90498910919832E-5	0.0	0.0	8.459739653738403E-4	4.007245099139244E-4
18	8.90498910919832E-5	0.0	0.0	0.0010685986931037983	4.007245099139244E-4
19	8.90498910919832E-5	0.0	0.0	0.0012912234208337563	4.45249455459916E-4
20	1.335748366379748E-4	0.0	0.0	0.0020481474951156136	4.45249455459916E-4
21	1.335748366379748E-4	0.0	0.0	0.0036065205892253194	4.45249455459916E-4
22	1.780997821839664E-4	0.0	0.0	0.006322542267530807	4.45249455459916E-4
23	1.780997821839664E-4	0.0	0.0	0.00837068976264642	4.45249455459916E-4
24	1.780997821839664E-4	0.0	0.0	0.011843635515233765	4.45249455459916E-4
25	1.780997821839664E-4	0.0	0.0	0.013847258064803386	4.45249455459916E-4
26	1.780997821839664E-4	0.0	0.0	0.017409253708482713	4.45249455459916E-4
27	1.780997821839664E-4	0.0	0.0	0.028763114822710573	4.45249455459916E-4
28	1.780997821839664E-4	0.0	0.0	0.10855181724112752	4.45249455459916E-4
29	1.780997821839664E-4	0.0	0.0	0.24604484908714958	4.45249455459916E-4
30	1.780997821839664E-4	0.0	0.0	0.4191133124244189	4.45249455459916E-4
31	1.780997821839664E-4	0.0	0.0	0.836935401428004	4.45249455459916E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATA	60	3.7283247E-5	21.583334	2
CGTATAC	95	3.609488E-6	17.526316	3
AACGCAG	6195	0.0	16.543987	5
AACGACG	135	2.2224413E-8	16.444443	15
CGTTACG	215	0.0	16.348837	24
CTAATAC	235	0.0	15.744679	3
CGCGCAA	130	2.591969E-7	15.653848	20
TAATACT	405	0.0	15.530864	4
CCTATAC	300	0.0	15.416667	3
TATCGTA	85	5.3669815E-4	15.235294	7
ATACCGT	85	5.3669815E-4	15.235294	6
TCGTTAC	260	0.0	14.942309	23
ACGCAGA	6930	0.0	14.842712	6
TATACCG	75	0.004106027	14.8	5
TTAGACT	250	0.0	14.799999	4
TATACTA	355	0.0	14.591549	5
ACTGCGT	165	1.6472768E-8	14.575759	8
CTCGTTA	305	0.0	14.557377	22
CTTATAC	2180	0.0	14.511469	37
CGCAGAG	7105	0.0	14.503168	7
>>END_MODULE