FastQCFastQC Report
Thu 9 Feb 2017
SRR2727398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366783
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT361021.5253616406742823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT124340.5253544579287581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT122710.5184674725143792No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91240.38550217742817994No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45310.19144129394203016No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43020.18176571320649168No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA42810.18087843287703181No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT31120.1314864945370995No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28440.12016310747542126No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG27940.11805053526242161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG852.7250904E-517.4117645
TATACCG1352.2226232E-816.4444455
GTATAAG3000.015.4166671
CTCGTTA3500.015.32857122
TCGTTAC2500.014.79999923
ATACCGT1651.6474587E-814.5757576
TTAATAC4400.014.2954543
AACGCAG176350.014.1097255
TAAGAGT3950.014.0506324
ACCCGAC6850.014.04379611
CGTTACG1854.9058144E-914.00000124
TAGGACT3600.013.8754
TTATACT4700.013.7765964
GCAATCG1356.5735367E-613.70370423
TAATACT3250.013.6615384
CCCGACT7500.013.56666812
TATACAC17700.013.4830537
TCGGTGA4950.013.45454613
GTACTAA1102.4580836E-413.4545451
AACGACG1652.5983172E-713.45454515
Produced by FastQC (version 0.11.5)