##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727398.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2366783
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78413483618904	31.0	31.0	34.0	30.0	34.0
2	31.911978833716482	31.0	31.0	34.0	30.0	34.0
3	31.9901909047006	33.0	31.0	34.0	30.0	34.0
4	35.66487041693303	37.0	35.0	37.0	33.0	37.0
5	35.29841730314946	37.0	35.0	37.0	33.0	37.0
6	35.309842938706254	37.0	35.0	37.0	32.0	37.0
7	35.27749354292303	37.0	35.0	37.0	32.0	37.0
8	35.20764176521464	37.0	35.0	37.0	32.0	37.0
9	36.98490736159589	39.0	37.0	39.0	33.0	39.0
10	36.72122201317146	38.0	37.0	39.0	32.0	39.0
11	36.87549766919908	39.0	37.0	39.0	33.0	39.0
12	36.77951506327365	38.0	37.0	39.0	32.0	39.0
13	36.898172329275646	39.0	37.0	39.0	33.0	39.0
14	37.88555140036074	39.0	37.0	40.0	33.0	41.0
15	37.783518810131724	39.0	37.0	40.0	33.0	41.0
16	37.746103888696176	39.0	37.0	40.0	33.0	41.0
17	37.67104250791053	39.0	37.0	40.0	33.0	41.0
18	37.650621117356344	39.0	37.0	40.0	32.0	41.0
19	37.70217675215683	39.0	37.0	40.0	32.0	41.0
20	37.647286210860905	39.0	37.0	40.0	32.0	41.0
21	37.579419406003844	39.0	37.0	40.0	32.0	41.0
22	37.520504837156594	39.0	37.0	40.0	32.0	41.0
23	37.412126925028616	39.0	36.0	40.0	32.0	41.0
24	37.34677323607614	39.0	36.0	40.0	32.0	41.0
25	37.210049252508576	39.0	36.0	40.0	32.0	41.0
26	37.0342921171903	39.0	36.0	40.0	31.0	41.0
27	36.893994506467216	39.0	36.0	40.0	31.0	41.0
28	36.735702005633804	39.0	35.0	40.0	31.0	41.0
29	36.582999793390435	38.0	35.0	40.0	30.0	41.0
30	36.44769165572002	38.0	35.0	40.0	30.0	41.0
31	36.28502486286237	38.0	35.0	40.0	30.0	41.0
32	36.114007494561186	38.0	35.0	40.0	30.0	41.0
33	35.94150794559535	38.0	35.0	40.0	30.0	41.0
34	35.750751547564775	38.0	35.0	40.0	29.0	41.0
35	35.605774166875456	38.0	34.0	40.0	28.0	41.0
36	35.429242140069455	38.0	34.0	40.0	27.0	41.0
37	35.267179542864724	38.0	34.0	40.0	26.0	41.0
38	35.096473145193286	38.0	34.0	40.0	25.0	41.0
39	34.91432252132959	38.0	34.0	40.0	25.0	41.0
40	34.71209274361021	38.0	33.0	40.0	24.0	41.0
41	34.52398846873583	38.0	33.0	40.0	24.0	41.0
42	34.318734332636325	38.0	33.0	40.0	23.0	41.0
43	32.65235849674431	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	15.0
11	6.0
12	8.0
13	10.0
14	9.0
15	25.0
16	48.0
17	92.0
18	185.0
19	417.0
20	901.0
21	1684.0
22	2814.0
23	4921.0
24	7821.0
25	12296.0
26	18153.0
27	25879.0
28	35779.0
29	47289.0
30	59597.0
31	72828.0
32	90253.0
33	115180.0
34	150726.0
35	204902.0
36	299791.0
37	440535.0
38	548480.0
39	226133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.38577596678698	18.362646681170176	13.756816742388297	23.494760609654538
2	16.522004763427827	23.73741065403968	36.28938521191001	23.451199370622486
3	19.13124270370372	25.161326577045717	30.632423842828004	25.075006876422552
4	13.74198648545304	15.900148006809243	34.46834796430429	35.88951754343343
5	14.726233879489586	37.842421548574585	33.6340086945022	13.79733587743363
6	33.92959979854511	35.9663306690981	15.332626607509011	14.771442924847777
7	28.285398365629632	30.774135186876023	22.59332604636758	18.34714040112676
8	27.64740155730373	35.646529487494206	20.311748056327936	16.394320898874128
9	26.244907116537515	14.240384521943922	19.87875525555152	39.63595310596704
10	15.036739743356279	28.51042955775836	34.24513358427874	22.20769711460662
11	34.984364853051595	22.07033766931738	22.39550478434229	20.549792693288737
12	20.47521889416985	26.757755146965312	31.627614360927893	21.139411597936945
13	31.46494630052692	19.3736392394233	25.751494750469305	23.409919709580475
14	21.06124642605596	20.311452296218118	29.33813535081163	29.289165926914297
15	24.563848903765155	28.891622087872022	23.76373330381366	22.78079570454917
16	22.607226771529117	26.449911124087	26.32577638085114	24.617085723532746
17	22.72747438189306	27.267603324850654	28.009369680279093	21.995552612977193
18	22.636084507958692	25.982736904904254	28.805175632916075	22.576002954220982
19	23.644964493998817	26.783824288073728	28.747798171611	20.823413046316457
20	22.65370336021511	26.963815440621296	28.384520253863577	21.99796094530001
21	21.980764607486194	27.822576045205665	28.533414343435794	21.663245003872344
22	22.371801724112434	27.15301740801755	27.952499236305144	22.52268163156487
23	21.708158289120718	26.82147032490938	28.453263353674586	23.017108032295315
24	21.94345658220462	26.934999955635984	28.64787350593612	22.473669956223276
25	22.297946199545965	26.802414923548124	28.375267187570643	22.524371689335272
26	22.00877731503057	27.151665361801232	29.006377010482158	21.833180312686036
27	22.72760113622584	27.444932636409845	28.490740384733197	21.336725842631115
28	22.13971454079229	27.772888346755913	28.411265418080156	21.67613169437164
29	21.90804987191475	27.788394626799327	28.75789626678914	21.54565923449678
30	21.736255499553614	27.794521086217028	28.76207915977088	21.707144254458477
31	21.77588735426949	27.08076743833296	28.79795063594761	22.34539457144994
32	21.23587164518251	27.52652017527589	28.704701698465808	22.53290648107579
33	21.926809513166184	27.053726514006566	28.446714379814285	22.572749593012965
34	22.111068061584017	27.243773510288015	28.751178287151802	21.89398014097617
35	22.01452351144993	27.299798925376766	28.535695921425834	22.14998164174747
36	21.69780668527702	27.202113586247663	28.83086451102615	22.26921521744917
37	21.30267117855756	26.999729168242293	28.787303271994098	22.91029638120605
38	21.397990436808108	26.986293208967616	29.15624288327236	22.45947347095192
39	21.092554746252613	26.95097100156626	29.33010757640223	22.626366675778893
40	21.4427769677237	27.057571395434227	29.458044949621492	22.041606687220586
41	21.199070637232058	26.723531477114715	29.40400535241296	22.673392533240268
42	20.700250086298574	27.0790351291183	29.27273011509716	22.947984669485965
43	20.631718243708868	26.51721767479317	28.957914603915945	23.893149477582018
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	691.0
1	864.0
2	1037.0
3	4458.5
4	7880.0
5	7880.0
6	10481.0
7	13082.0
8	12719.5
9	12357.0
10	14889.0
11	17421.0
12	17421.0
13	25844.0
14	34267.0
15	52289.5
16	70312.0
17	57071.0
18	43830.0
19	43830.0
20	43546.0
21	43262.0
22	33876.5
23	24491.0
24	26149.5
25	27808.0
26	27808.0
27	31985.5
28	36163.0
29	40069.0
30	43975.0
31	49046.5
32	54118.0
33	54118.0
34	60922.0
35	67726.0
36	76862.0
37	85998.0
38	96937.5
39	107877.0
40	107877.0
41	118048.0
42	128219.0
43	138954.5
44	149690.0
45	161515.0
46	173340.0
47	173340.0
48	184050.0
49	194760.0
50	196172.5
51	197585.0
52	195443.0
53	193301.0
54	193301.0
55	179995.0
56	166689.0
57	148770.5
58	130852.0
59	118862.0
60	106872.0
61	106872.0
62	92865.0
63	78858.0
64	66674.5
65	54491.0
66	45522.0
67	36553.0
68	36553.0
69	30050.5
70	23548.0
71	19921.5
72	16295.0
73	13059.5
74	9824.0
75	9824.0
76	7824.0
77	5824.0
78	4604.0
79	3384.0
80	2710.5
81	2037.0
82	2037.0
83	1650.0
84	1263.0
85	961.0
86	659.0
87	470.5
88	282.0
89	282.0
90	191.0
91	100.0
92	69.5
93	39.0
94	26.0
95	13.0
96	13.0
97	10.0
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2366783.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.13762201366631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.14673522529232	48.92091086212106
2	8.249209534778725	9.591788516889952
3	2.7446990090295156	4.787108205847275
4	1.3346585522019176	3.103754977008889
5	0.7842363417870093	2.279681800409678
6	0.5128793975061597	1.7890553130486018
7	0.3845289006985885	1.5648917117501617
8	0.2781460403919281	1.2936599488723102
9	0.2194268498630918	1.1481259731290945
>10	1.2357641395560237	12.93130825385912
>50	0.06798807462847532	2.717149320207751
>100	0.03765361991459172	4.184532290709955
>500	0.002473690856350015	1.0648195068593707
>1k	0.0013096010415970065	1.6652450609972658
>5k	7.275561342205591E-5	0.3859304512824571
>10k+	2.1826684026616774E-4	2.5720378070070553
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36102	1.5253616406742823	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12434	0.5253544579287581	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12271	0.5184674725143792	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9124	0.38550217742817994	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4531	0.19144129394203016	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4302	0.18176571320649168	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	4281	0.18087843287703181	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	3112	0.1314864945370995	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2844	0.12016310747542126	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	2794	0.11805053526242161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.2251444259993416E-5	0.0
4	0.0	0.0	0.0	4.2251444259993416E-5	0.0
5	0.0	0.0	0.0	4.2251444259993416E-5	0.0
6	0.0	0.0	0.0	4.2251444259993416E-5	4.2251444259993416E-5
7	0.0	0.0	0.0	4.2251444259993416E-5	4.2251444259993416E-5
8	4.2251444259993416E-5	0.0	0.0	4.2251444259993416E-5	4.2251444259993416E-5
9	8.450288851998683E-5	0.0	0.0	4.2251444259993416E-5	4.2251444259993416E-5
10	2.535086655599605E-4	0.0	0.0	1.6900577703997366E-4	4.2251444259993416E-5
11	2.535086655599605E-4	0.0	0.0	5.492687753799145E-4	4.2251444259993416E-5
12	2.535086655599605E-4	0.0	0.0	0.0013520462163197893	4.2251444259993416E-5
13	2.535086655599605E-4	0.0	0.0	0.0014788005490997695	4.2251444259993416E-5
14	2.535086655599605E-4	0.0	0.0	0.0018168121031797168	4.2251444259993416E-5
15	2.535086655599605E-4	0.0	0.0	0.0026195895441195917	4.2251444259993416E-5
16	2.535086655599605E-4	0.0	0.0	0.004182892981739348	8.450288851998683E-5
17	2.535086655599605E-4	0.0	0.0	0.005999705084919065	8.450288851998683E-5
18	2.957601098199539E-4	0.0	0.0	0.0069292368586389205	8.450288851998683E-5
19	2.957601098199539E-4	0.0	0.0	0.009253066292938558	8.450288851998683E-5
20	2.957601098199539E-4	0.0	0.0	0.013942976605797828	8.450288851998683E-5
21	2.957601098199539E-4	0.0	0.0	0.020069436023496874	8.450288851998683E-5
22	2.957601098199539E-4	0.0	0.0	0.032660366412974914	8.450288851998683E-5
23	2.957601098199539E-4	0.0	0.0	0.048673663787512415	1.6900577703997366E-4
24	2.957601098199539E-4	0.0	0.0	0.07732014299578796	1.6900577703997366E-4
25	2.957601098199539E-4	0.0	0.0	0.10516384476312361	1.6900577703997366E-4
26	2.957601098199539E-4	0.0	0.0	0.12903591077001988	1.6900577703997366E-4
27	2.957601098199539E-4	0.0	0.0	0.16511864416805427	1.6900577703997366E-4
28	2.957601098199539E-4	0.0	0.0	0.31113963553059154	1.6900577703997366E-4
29	2.957601098199539E-4	0.0	0.0	0.5717887951704909	1.6900577703997366E-4
30	2.957601098199539E-4	0.0	0.0	0.8836467052535023	1.6900577703997366E-4
31	2.957601098199539E-4	0.0	0.0	1.4492245381177742	1.6900577703997366E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	85	2.7250904E-5	17.411764	5
TATACCG	135	2.2226232E-8	16.444445	5
GTATAAG	300	0.0	15.416667	1
CTCGTTA	350	0.0	15.328571	22
TCGTTAC	250	0.0	14.799999	23
ATACCGT	165	1.6474587E-8	14.575757	6
TTAATAC	440	0.0	14.295454	3
AACGCAG	17635	0.0	14.109725	5
TAAGAGT	395	0.0	14.050632	4
ACCCGAC	685	0.0	14.043796	11
CGTTACG	185	4.9058144E-9	14.000001	24
TAGGACT	360	0.0	13.875	4
TTATACT	470	0.0	13.776596	4
GCAATCG	135	6.5735367E-6	13.703704	23
TAATACT	325	0.0	13.661538	4
CCCGACT	750	0.0	13.566668	12
TATACAC	1770	0.0	13.48305	37
TCGGTGA	495	0.0	13.454546	13
GTACTAA	110	2.4580836E-4	13.454545	1
AACGACG	165	2.5983172E-7	13.454545	15
>>END_MODULE