FastQCFastQC Report
Thu 9 Feb 2017
SRR2727397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061323
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71270.6715203571391556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30470.2870945037467387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29940.2821007365335529No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19110.18005828574335994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA502.7019126E-422.221
AGGGCGT1457.2759576E-1219.1379310
GTCTAAA1258.576535E-917.7600021
ATTGCGA852.7227967E-517.41176410
ACGAGCG1005.8769347E-616.6500025
CTCGTTA1352.2186214E-816.44444522
ATTAGAC904.445439E-516.4444453
TTTCGAG803.3822595E-416.187512
CGTTACG803.3822595E-416.187524
TAACCCG700.00259231815.8571425
GTCTTAT1455.3432814E-815.3103451
CATTGCG855.363669E-415.2352949
TAAGCCG855.363669E-415.2352945
CTTATTC1353.9709994E-715.0740753
CTTATAC7500.015.04666637
TAATACT2350.014.9574474
ATAATAC1508.097777E-814.83
GCGTAGA908.273276E-414.3888891
CTATACT2101.3460522E-1014.0952384
GTATAGA1051.6554426E-414.0952381
Produced by FastQC (version 0.11.5)