##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727397.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1061323 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40113707137224 33.0 31.0 34.0 31.0 34.0 2 32.53988559561981 34.0 31.0 34.0 31.0 34.0 3 32.634519368750134 34.0 31.0 34.0 31.0 34.0 4 36.14869083210295 37.0 35.0 37.0 35.0 37.0 5 35.962804914243826 37.0 35.0 37.0 35.0 37.0 6 35.97858333419703 37.0 35.0 37.0 35.0 37.0 7 35.93708701309592 37.0 35.0 37.0 35.0 37.0 8 35.8957047006425 37.0 35.0 37.0 35.0 37.0 9 37.73120247087833 39.0 37.0 39.0 35.0 39.0 10 37.562581796493625 39.0 37.0 39.0 35.0 39.0 11 37.670569656928194 39.0 37.0 39.0 35.0 39.0 12 37.596727857589066 39.0 37.0 39.0 35.0 39.0 13 37.67135358415864 39.0 37.0 39.0 35.0 39.0 14 38.93365733146271 40.0 38.0 41.0 36.0 41.0 15 38.85944147069271 40.0 38.0 41.0 36.0 41.0 16 38.831268143628286 40.0 38.0 41.0 35.0 41.0 17 38.76659226267592 40.0 38.0 41.0 35.0 41.0 18 38.74084044159978 40.0 38.0 41.0 35.0 41.0 19 38.78743982746063 40.0 38.0 41.0 35.0 41.0 20 38.755847183185516 40.0 38.0 41.0 35.0 41.0 21 38.709435299150215 40.0 38.0 41.0 35.0 41.0 22 38.66953886799777 40.0 38.0 41.0 34.0 41.0 23 38.61247424205449 40.0 38.0 41.0 34.0 41.0 24 38.56599169150202 40.0 38.0 41.0 34.0 41.0 25 38.45421704796749 40.0 38.0 41.0 34.0 41.0 26 38.35765926112974 40.0 38.0 41.0 34.0 41.0 27 38.26903496861936 40.0 38.0 41.0 34.0 41.0 28 38.178553560037805 40.0 38.0 41.0 34.0 41.0 29 38.09913099028288 40.0 38.0 41.0 33.0 41.0 30 38.03330277399058 40.0 37.0 41.0 33.0 41.0 31 37.9406429522398 40.0 37.0 41.0 33.0 41.0 32 37.8422742181221 40.0 37.0 41.0 33.0 41.0 33 37.74101569456235 40.0 37.0 41.0 33.0 41.0 34 37.646740907339236 40.0 37.0 41.0 33.0 41.0 35 37.57622231874745 40.0 37.0 41.0 33.0 41.0 36 37.48394315396915 40.0 37.0 41.0 32.0 41.0 37 37.3943973700749 40.0 37.0 41.0 32.0 41.0 38 37.30142944230927 40.0 37.0 41.0 32.0 41.0 39 37.2057705335699 40.0 36.0 41.0 31.0 41.0 40 37.08549046802906 39.0 36.0 41.0 31.0 41.0 41 36.99096222356436 39.0 36.0 41.0 31.0 41.0 42 36.8672194986823 39.0 36.0 41.0 31.0 41.0 43 35.21128063746852 38.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 1.0 10 2.0 11 1.0 12 1.0 13 2.0 14 2.0 15 2.0 16 4.0 17 5.0 18 13.0 19 39.0 20 98.0 21 194.0 22 391.0 23 759.0 24 1232.0 25 2101.0 26 3345.0 27 4853.0 28 7332.0 29 10125.0 30 13956.0 31 18034.0 32 23404.0 33 30572.0 34 41982.0 35 58591.0 36 87110.0 37 147568.0 38 288262.0 39 321341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4647859322751 17.529818914694207 11.977880437906274 26.027514715124422 2 16.49413043908405 20.658932294881012 37.40538931126528 25.44154795476966 3 18.21019614198505 22.92016662222528 30.722032783610643 28.147604452179024 4 12.920194888832146 14.979040311008054 36.15035196636651 35.950412833793294 5 14.218480142237567 37.28525623208015 34.968807799322164 13.527455826360118 6 34.993117081227865 36.585940378188354 14.807838895416381 13.6131036451674 7 29.87064258477391 30.888899986149365 21.33714241564538 17.90331501343135 8 27.556361258542406 36.430945150533816 19.19170695443329 16.820986636490492 9 26.994421114024664 13.468849728122354 18.536015897139702 41.000713260713276 10 14.390812222103921 27.854008628852856 35.19352732391553 22.561651825127694 11 36.92975653971505 20.934060601720685 22.225561869478 19.910620989086265 12 20.846151454364033 25.234636392502562 31.98960165755383 21.929610495579574 13 31.391103368154656 19.190105180044153 25.064377197139798 24.3544142546614 14 21.45746393887629 19.299120060528228 27.5874545260962 31.65596147449928 15 24.851341203384834 28.289125930560253 22.60471128958856 24.254821576466355 16 24.1397764865173 26.352015361958614 24.886297573877133 24.621910577646954 17 23.718038712060324 26.771868695957778 26.31008656177243 23.200006030209465 18 23.658113505502097 25.977011710855223 26.96813316963827 23.39674161400441 19 24.68739488355571 25.904837641321254 26.79052465649006 22.61724281863297 20 23.94775200386687 25.837751561023364 26.785719333322653 23.42877710178711 21 23.560593711810636 26.143502025302386 26.93251724498574 23.363387017901243 22 23.844202000710435 25.659012383600466 26.55063538621136 23.946150229477738 23 23.47023479185884 25.481874980566708 26.725605682718644 24.32228454485581 24 23.383456308776875 25.785458338319252 26.832830344767807 23.99825500813607 25 23.874353048035328 25.459638583164594 26.624882340248917 24.041126028551158 26 23.632767781344604 25.7626566087798 26.88644267579238 23.718132934083215 27 23.993732351037337 26.22453296498804 26.429654308820215 23.352080375154408 28 23.706449403244818 26.141523362821683 26.627709000935624 23.52431823299787 29 23.620895806460425 26.271267088341627 26.711001269170648 23.3968358360273 30 23.648502859167284 26.28935771673656 26.63892142165957 23.42321800243658 31 23.602616734019712 25.586555647997827 26.867880937282994 23.942946680699468 32 23.21470466578035 26.13398560099046 26.777239351262526 23.874070381966657 33 23.443664181403776 25.707630947411864 26.750668740807466 24.098036130376897 34 23.68751077664387 25.756814843360598 27.144045686374458 23.411628693621076 35 23.682611231453574 25.83878800327516 26.851768971368756 23.626831793902518 36 23.451390387280778 25.789132997211972 27.02721037799049 23.73226623751676 37 23.39787227827909 25.285233618794656 27.057361425315385 24.259532677610867 38 23.1928451564698 25.42553021087831 27.373664756158117 24.00795987649377 39 23.017121083779397 25.283160734291073 27.603566492010444 24.09615168991909 40 23.194352708836046 25.19883202380425 27.92495781208925 23.68185745527045 41 22.940989689284034 25.058723875766383 27.9934572227305 24.00682921221909 42 22.588222435582757 25.15991832835056 28.074959272530602 24.176899963536076 43 22.36463357526408 24.62982522756974 28.13601514336352 24.86952605380266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 100.0 1 145.5 2 191.0 3 896.0 4 1601.0 5 1601.0 6 1998.5 7 2396.0 8 2071.5 9 1747.0 10 2312.5 11 2878.0 12 2878.0 13 4370.0 14 5862.0 15 9273.0 16 12684.0 17 10694.0 18 8704.0 19 8704.0 20 9341.5 21 9979.0 22 8747.5 23 7516.0 24 8929.0 25 10342.0 26 10342.0 27 12456.5 28 14571.0 29 16860.0 30 19149.0 31 22140.5 32 25132.0 33 25132.0 34 28893.5 35 32655.0 36 37242.0 37 41829.0 38 46998.5 39 52168.0 40 52168.0 41 57355.5 42 62543.0 43 67333.0 44 72123.0 45 77328.0 46 82533.0 47 82533.0 48 86925.0 49 91317.0 50 92600.0 51 93883.0 52 92897.5 53 91912.0 54 91912.0 55 86471.5 56 81031.0 57 74056.0 58 67081.0 59 60684.5 60 54288.0 61 54288.0 62 47380.5 63 40473.0 64 33983.5 65 27494.0 66 22971.5 67 18449.0 68 18449.0 69 14950.0 70 11451.0 71 9372.0 72 7293.0 73 5823.5 74 4354.0 75 4354.0 76 3425.0 77 2496.0 78 1987.5 79 1479.0 80 1127.0 81 775.0 82 775.0 83 620.0 84 465.0 85 353.0 86 241.0 87 165.5 88 90.0 89 90.0 90 58.0 91 26.0 92 20.0 93 14.0 94 10.0 95 6.0 96 6.0 97 4.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 1061323.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.00813324982258 #Duplication Level Percentage of deduplicated Percentage of total 1 86.39414213416214 59.618984723983715 2 7.421220046723283 10.242490837210697 3 2.319461806187921 4.801851881678706 4 1.1290494695315652 3.1165438495630298 5 0.6616710587480135 2.283034229481705 6 0.4704583806157204 1.947927277081523 7 0.31275411044243534 1.5107804129488913 8 0.2358693590498271 1.3021523327088569 9 0.1725045083531935 1.0713792688769077 >10 0.840970415204446 9.899567400040217 >50 0.03286845587968995 1.5205051811918533 >100 0.007798858456433585 0.8926306712257365 >500 6.841092474043567E-4 0.3684198328064716 >1k 4.10465548442614E-4 0.7508135598352287 >5k 1.3682184948087133E-4 0.6729185413664077 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 7127 0.6715203571391556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3047 0.2870945037467387 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 2994 0.2821007365335529 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1911 0.18005828574335994 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 9.422202289029824E-5 0.0 12 0.0 0.0 0.0 9.422202289029824E-5 0.0 13 0.0 0.0 0.0 1.8844404578059648E-4 0.0 14 0.0 0.0 0.0 1.8844404578059648E-4 0.0 15 0.0 0.0 0.0 2.826660686708947E-4 0.0 16 0.0 0.0 0.0 7.537761831223859E-4 0.0 17 0.0 0.0 0.0 0.0010364422517932807 0.0 18 0.0 0.0 0.0 0.0013191083204641754 0.0 19 0.0 0.0 0.0 0.0016959964120253684 0.0 20 0.0 0.0 0.0 0.0024497725951477542 0.0 21 0.0 0.0 0.0 0.0034862148469410347 0.0 22 0.0 0.0 0.0 0.0053706553047469996 0.0 23 0.0 0.0 0.0 0.008479982060126841 0.0 24 0.0 0.0 0.0 0.016206187937131297 0.0 25 0.0 0.0 0.0 0.02346128369968426 0.0 26 0.0 0.0 0.0 0.02836082888997977 9.422202289029824E-5 27 0.0 0.0 0.0 0.037688809156119295 9.422202289029824E-5 28 0.0 0.0 0.0 0.08103093968565649 9.422202289029824E-5 29 0.0 0.0 0.0 0.1618734353255324 9.422202289029824E-5 30 0.0 0.0 0.0 0.26099500340612614 9.422202289029824E-5 31 0.0 0.0 0.0 0.5819152133704819 9.422202289029824E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGTA 50 2.7019126E-4 22.2 21 AGGGCGT 145 7.2759576E-12 19.13793 10 GTCTAAA 125 8.576535E-9 17.760002 1 ATTGCGA 85 2.7227967E-5 17.411764 10 ACGAGCG 100 5.8769347E-6 16.650002 5 CTCGTTA 135 2.2186214E-8 16.444445 22 ATTAGAC 90 4.445439E-5 16.444445 3 TTTCGAG 80 3.3822595E-4 16.1875 12 CGTTACG 80 3.3822595E-4 16.1875 24 TAACCCG 70 0.002592318 15.857142 5 GTCTTAT 145 5.3432814E-8 15.310345 1 CATTGCG 85 5.363669E-4 15.235294 9 TAAGCCG 85 5.363669E-4 15.235294 5 CTTATTC 135 3.9709994E-7 15.074075 3 CTTATAC 750 0.0 15.046666 37 TAATACT 235 0.0 14.957447 4 ATAATAC 150 8.097777E-8 14.8 3 GCGTAGA 90 8.273276E-4 14.388889 1 CTATACT 210 1.3460522E-10 14.095238 4 GTATAGA 105 1.6554426E-4 14.095238 1 >>END_MODULE