FastQCFastQC Report
Thu 9 Feb 2017
SRR2727396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872056
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62550.7172704505215262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25910.29711394681075526No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24760.2839267203023659No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17400.19952847064867393No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9970.11432752025099305No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.10733255662480391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC701.9218169E-723.7857133
ATAAGTC450.003825078520.5555573
TAGACAC1002.8729482E-718.55
GTAGCGC500.007033554418.58
TCTAAAC1002.8729482E-718.53
GTATAGG1103.848436E-818.51
GTTATAT701.21876954E-418.51
GACTATT609.2343806E-418.57
CTTATAC6350.018.06299237
TAACGCA953.6043675E-617.5263164
CGATCGC752.0665463E-417.26666619
TATACCG650.00157952117.0769235
TAAGAGT1757.2759576E-1216.9142864
TTAGGGT1005.874519E-616.6500024
GTATTAC1005.874519E-616.6500021
TGGACCG904.4439766E-516.4444455
GGACCGA803.381394E-416.18756
TTACACC2200.015.9772734
TGGACTG3850.015.8571425
GAATAGG1059.337848E-615.8571421
Produced by FastQC (version 0.11.5)