##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727396.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	872056
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.361515774216336	33.0	31.0	34.0	31.0	34.0
2	32.5001605401488	34.0	31.0	34.0	31.0	34.0
3	32.59551106809654	34.0	31.0	34.0	31.0	34.0
4	36.115607254579984	37.0	35.0	37.0	35.0	37.0
5	35.92989326373536	37.0	35.0	37.0	35.0	37.0
6	35.950676332712575	37.0	35.0	37.0	35.0	37.0
7	35.911350876549214	37.0	35.0	37.0	35.0	37.0
8	35.86121189464897	37.0	35.0	37.0	35.0	37.0
9	37.69554822167384	39.0	37.0	39.0	35.0	39.0
10	37.52644440265304	39.0	37.0	39.0	35.0	39.0
11	37.634245965855406	39.0	37.0	39.0	35.0	39.0
12	37.55583357032117	39.0	37.0	39.0	35.0	39.0
13	37.638850027979856	39.0	37.0	39.0	35.0	39.0
14	38.88612084545029	40.0	38.0	41.0	36.0	41.0
15	38.81294549891292	40.0	38.0	41.0	35.0	41.0
16	38.784746621776584	40.0	38.0	41.0	35.0	41.0
17	38.72552680103113	40.0	38.0	41.0	35.0	41.0
18	38.69586012824865	40.0	38.0	41.0	35.0	41.0
19	38.743201124698416	40.0	38.0	41.0	35.0	41.0
20	38.70651196712138	40.0	38.0	41.0	35.0	41.0
21	38.657143577935365	40.0	38.0	41.0	34.0	41.0
22	38.618750401350376	40.0	38.0	41.0	34.0	41.0
23	38.55464442650472	40.0	38.0	41.0	34.0	41.0
24	38.51449677543644	40.0	38.0	41.0	34.0	41.0
25	38.39780472813673	40.0	38.0	41.0	34.0	41.0
26	38.297704505215265	40.0	38.0	41.0	34.0	41.0
27	38.20058344876934	40.0	38.0	41.0	34.0	41.0
28	38.114558010036056	40.0	38.0	41.0	34.0	41.0
29	38.02400304567597	40.0	37.0	41.0	33.0	41.0
30	37.952902107204125	40.0	37.0	41.0	33.0	41.0
31	37.8561067179172	40.0	37.0	41.0	33.0	41.0
32	37.75207555478089	40.0	37.0	41.0	33.0	41.0
33	37.6548937224215	40.0	37.0	41.0	33.0	41.0
34	37.55190033667563	40.0	37.0	41.0	33.0	41.0
35	37.46475685047749	40.0	37.0	41.0	32.0	41.0
36	37.369495766326935	40.0	37.0	41.0	32.0	41.0
37	37.27861169466181	40.0	36.0	41.0	32.0	41.0
38	37.18732856605539	39.0	36.0	41.0	31.0	41.0
39	37.07929421963727	39.0	36.0	41.0	31.0	41.0
40	36.964033273092554	39.0	36.0	41.0	31.0	41.0
41	36.8571766033374	39.0	36.0	41.0	31.0	41.0
42	36.73411340556111	39.0	36.0	41.0	30.0	41.0
43	35.071858917317236	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	1.0
12	0.0
13	1.0
14	1.0
15	1.0
16	6.0
17	6.0
18	15.0
19	36.0
20	91.0
21	168.0
22	373.0
23	646.0
24	1099.0
25	1837.0
26	2889.0
27	4226.0
28	6416.0
29	9122.0
30	11942.0
31	15408.0
32	20093.0
33	26200.0
34	35490.0
35	49020.0
36	73089.0
37	122834.0
38	235956.0
39	255087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.57362829910006	17.584306512425808	12.082022255451484	25.760042933022646
2	16.605814305503316	20.969295549827073	37.04544203583256	25.37944810883705
3	18.27738126909281	23.189909822304987	30.660301632005282	27.872407276596917
4	13.20970212922106	14.957410991954646	35.69747814360545	36.13540873521884
5	14.219155650554551	37.372026567101194	34.867600245855776	13.541217536488482
6	35.10382360765823	36.20145953929564	14.93401799884411	13.760698854202024
7	29.876865705872103	30.832538277358335	21.488642931187904	17.80195308558166
8	27.608433403359417	36.27771610997459	19.360683258873284	16.753167227792712
9	27.006178502298017	13.426431330098069	18.705794123313183	40.861596044290735
10	14.439439669012083	27.91919326281798	35.057954993716	22.583412074453936
11	36.81357619235462	20.97330905354702	22.290655645967693	19.92245910813067
12	20.6892676617098	25.181295696606643	31.977762895960808	22.151673745722753
13	31.328836680213197	19.2073674167714	25.17372737530617	24.29006852770923
14	21.54643738475511	19.271583476290512	27.60132376819837	31.580655370756006
15	24.97695102149403	28.23442531213592	22.671938499362426	24.116685167007624
16	24.077696845156733	26.314479804049277	24.9576862036383	24.650137147155686
17	23.580939756162447	26.737847110736006	26.40851046263084	23.272702670470704
18	23.546996981845204	25.904758410010366	27.12773032924491	23.42051427889952
19	24.533172181603017	26.021264689423617	26.909625069949637	22.53593805902373
20	23.899153265386627	25.859119139137853	26.839102076013464	23.402625519462052
21	23.439435082150688	26.254277248250112	27.020168429550395	23.286119240048805
22	23.854202023723246	25.650187602631025	26.519168493766454	23.976441879879275
23	23.26719843679764	25.71864650893979	26.8645591567514	24.14959589751117
24	23.395401212766153	25.729998990890497	26.851715944847577	24.022883851495774
25	23.799962387736567	25.55810636014201	26.75286908180209	23.889062170319335
26	23.36833873054024	25.859004467602997	27.030718210757108	23.741938591099654
27	23.80558129294449	26.304159365912284	26.641981707596763	23.24827763354647
28	23.646187859495264	26.30129257754089	26.610332363976624	23.44218719898722
29	23.457438513123012	26.413097324025063	26.900566018696047	23.22889814415588
30	23.72473786087132	26.30966359958535	26.676153824983718	23.289444714559615
31	23.618781362664784	25.643421982074543	26.941159742034916	23.796636913225754
32	23.066867265404976	26.26310695643399	26.861692368380012	23.808333409781024
33	23.373154935004173	25.690093296760757	26.881301200840312	24.055450567394754
34	23.684602823671874	25.75568541469814	27.23357215591659	23.326139605713394
35	23.568784573467756	25.84501454035062	26.996431421835293	23.58976946434633
36	23.455718440100178	25.678052785600926	27.126468942361498	23.7397598319374
37	23.09438843377031	25.33266212261598	27.36716449402332	24.20578494959039
38	23.162617997009367	25.393208693019716	27.42083077233572	24.023342537635198
39	22.786839377287695	25.286678837138897	27.864380269157024	24.062101516416377
40	23.22683405652848	25.09712679002266	28.085466988358547	23.590572165090315
41	22.843716458576054	25.00986175199758	28.135693120625284	24.010728668801086
42	22.40372177933527	25.238746135569272	28.244057721063786	24.113474364031667
43	22.162452869999175	24.720086783417578	28.165393048152872	24.952067298430375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	111.0
1	153.5
2	196.0
3	960.5
4	1725.0
5	1725.0
6	2044.0
7	2363.0
8	2049.0
9	1735.0
10	2238.5
11	2742.0
12	2742.0
13	4169.5
14	5597.0
15	8521.5
16	11446.0
17	9677.5
18	7909.0
19	7909.0
20	8353.0
21	8797.0
22	7533.5
23	6270.0
24	7261.5
25	8253.0
26	8253.0
27	9944.0
28	11635.0
29	13451.0
30	15267.0
31	17545.0
32	19823.0
33	19823.0
34	22754.0
35	25685.0
36	29308.5
37	32932.0
38	37423.5
39	41915.0
40	41915.0
41	46028.5
42	50142.0
43	54284.0
44	58426.0
45	62813.0
46	67200.0
47	67200.0
48	71118.0
49	75036.0
50	76535.5
51	78035.0
52	77267.0
53	76499.0
54	76499.0
55	72186.5
56	67874.0
57	61919.5
58	55965.0
59	50724.5
60	45484.0
61	45484.0
62	39542.0
63	33600.0
64	28054.0
65	22508.0
66	18532.5
67	14557.0
68	14557.0
69	11850.5
70	9144.0
71	7396.5
72	5649.0
73	4483.0
74	3317.0
75	3317.0
76	2614.0
77	1911.0
78	1494.0
79	1077.0
80	839.0
81	601.0
82	601.0
83	483.0
84	365.0
85	266.0
86	167.0
87	119.5
88	72.0
89	72.0
90	46.0
91	20.0
92	11.5
93	3.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	872056.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.54070184419518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.52686587716958	58.62071413755551
2	7.85543134367763	10.768335551691079
3	2.568401893257938	5.281202051455762
4	1.24219926758678	3.4056483852297244
5	0.7068708348141999	2.422471156567871
6	0.48088199770347995	1.9775993776101102
7	0.3479630523688058	1.6694742277644474
8	0.23820794502935452	1.3061551789740338
9	0.17397037670981846	1.0731646547810987
>10	0.8250809291212033	9.534310097941043
>50	0.024927036847184766	1.1397383482982493
>100	0.0076940819252544175	0.8740381773597397
>500	8.363132160254951E-4	0.42968192493057406
>1k	5.01787929615297E-4	0.7803748300433344
>5k	1.6726264320509902E-4	0.7170918997974227
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6255	0.7172704505215262	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2591	0.29711394681075526	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2476	0.2839267203023659	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1740	0.19952847064867393	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	997	0.11432752025099305	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	936	0.10733255662480391	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2934306971111946E-4
2	0.0	0.0	0.0	0.0	2.2934306971111946E-4
3	0.0	0.0	0.0	0.0	2.2934306971111946E-4
4	0.0	0.0	0.0	0.0	2.2934306971111946E-4
5	0.0	0.0	0.0	0.0	2.2934306971111946E-4
6	0.0	0.0	0.0	0.0	2.2934306971111946E-4
7	0.0	0.0	0.0	0.0	2.2934306971111946E-4
8	0.0	0.0	0.0	1.1467153485555973E-4	2.2934306971111946E-4
9	0.0	0.0	0.0	1.1467153485555973E-4	3.440146045666792E-4
10	0.0	0.0	0.0	1.1467153485555973E-4	3.440146045666792E-4
11	0.0	0.0	0.0	2.2934306971111946E-4	3.440146045666792E-4
12	0.0	0.0	0.0	3.440146045666792E-4	3.440146045666792E-4
13	0.0	0.0	0.0	3.440146045666792E-4	3.440146045666792E-4
14	0.0	0.0	0.0	5.733576742777987E-4	3.440146045666792E-4
15	0.0	0.0	0.0	6.880292091333584E-4	3.440146045666792E-4
16	0.0	0.0	0.0	0.0010320438137000375	3.440146045666792E-4
17	0.0	0.0	0.0	0.0017200730228333961	3.440146045666792E-4
18	0.0	0.0	0.0	0.002178759162255635	3.440146045666792E-4
19	0.0	0.0	0.0	0.002981459906244553	3.440146045666792E-4
20	0.0	0.0	0.0	0.0035548175805223516	3.440146045666792E-4
21	0.0	0.0	0.0	0.004930875998789068	3.440146045666792E-4
22	0.0	0.0	0.0	0.007109635161044703	3.440146045666792E-4
23	0.0	0.0	0.0	0.010664452741567056	3.440146045666792E-4
24	0.0	0.0	0.0	0.02155824855284523	3.440146045666792E-4
25	0.0	0.0	0.0	0.032337372829267845	3.440146045666792E-4
26	0.0	0.0	0.0	0.04036438026915703	3.440146045666792E-4
27	0.0	0.0	0.0	0.05194620528956856	3.440146045666792E-4
28	0.0	0.0	0.0	0.09930554918491473	3.440146045666792E-4
29	0.0	0.0	0.0	0.18588255800086234	3.440146045666792E-4
30	0.0	0.0	0.0	0.29206839927711065	3.440146045666792E-4
31	0.0	0.0	0.0	0.6335602300769675	3.440146045666792E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGAC	70	1.9218169E-7	23.785713	3
ATAAGTC	45	0.0038250785	20.555557	3
TAGACAC	100	2.8729482E-7	18.5	5
GTAGCGC	50	0.0070335544	18.5	8
TCTAAAC	100	2.8729482E-7	18.5	3
GTATAGG	110	3.848436E-8	18.5	1
GTTATAT	70	1.21876954E-4	18.5	1
GACTATT	60	9.2343806E-4	18.5	7
CTTATAC	635	0.0	18.062992	37
TAACGCA	95	3.6043675E-6	17.526316	4
CGATCGC	75	2.0665463E-4	17.266666	19
TATACCG	65	0.001579521	17.076923	5
TAAGAGT	175	7.2759576E-12	16.914286	4
TTAGGGT	100	5.874519E-6	16.650002	4
GTATTAC	100	5.874519E-6	16.650002	1
TGGACCG	90	4.4439766E-5	16.444445	5
GGACCGA	80	3.381394E-4	16.1875	6
TTACACC	220	0.0	15.977273	4
TGGACTG	385	0.0	15.857142	5
GAATAGG	105	9.337848E-6	15.857142	1
>>END_MODULE