FastQCFastQC Report
Thu 9 Feb 2017
SRR2727395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005244
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62660.6233312509201746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25650.25516193083470284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23500.2337740886789675No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.15359455017886206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCG450.003825479720.55555510
TATACTA1052.2586391E-819.380955
CTATCGG500.007034283618.51
TCTAGGT902.1516698E-618.53
CTTATAC5750.018.01739137
CACGATC752.0669407E-417.26666626
CTAATCT650.001579747317.0769234
CTAAGAC1757.2759576E-1216.9142863
CCCCTAT904.4450644E-516.4444451
TAAGACT1606.2937033E-1016.18754
GTAGCGT700.002592189715.85714324
ATACCGG700.002592189715.8571436
CTTACAC2450.015.8571423
TAGACTC1302.5878217E-715.6538465
GTCTTAC1302.5878217E-715.6538461
AACGCAG24650.015.6105475
ATAATAC957.058267E-515.5789483
GGTATAT957.058267E-515.5789481
GTGTAGA2055.456968E-1215.3414631
ACCGTCC1455.342372E-815.3103468
Produced by FastQC (version 0.11.5)