##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727395.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1005244
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38054641460183	33.0	31.0	34.0	31.0	34.0
2	32.52134208212136	34.0	31.0	34.0	31.0	34.0
3	32.613650019298795	34.0	31.0	34.0	31.0	34.0
4	36.13739947714187	37.0	35.0	37.0	35.0	37.0
5	35.95469060248059	37.0	35.0	37.0	35.0	37.0
6	35.96958549367119	37.0	35.0	37.0	35.0	37.0
7	35.93177477308992	37.0	35.0	37.0	35.0	37.0
8	35.8811880498665	37.0	35.0	37.0	35.0	37.0
9	37.7216576274019	39.0	37.0	39.0	35.0	39.0
10	37.54590029883292	39.0	37.0	39.0	35.0	39.0
11	37.65703948494097	39.0	37.0	39.0	35.0	39.0
12	37.579358842231336	39.0	37.0	39.0	35.0	39.0
13	37.65385219906808	39.0	37.0	39.0	35.0	39.0
14	38.9181850376625	40.0	38.0	41.0	36.0	41.0
15	38.84810652936004	40.0	38.0	41.0	35.0	41.0
16	38.8199621186498	40.0	38.0	41.0	35.0	41.0
17	38.754475530319006	40.0	38.0	41.0	35.0	41.0
18	38.71666878887116	40.0	38.0	41.0	35.0	41.0
19	38.76851092868995	40.0	38.0	41.0	35.0	41.0
20	38.7331006203469	40.0	38.0	41.0	35.0	41.0
21	38.69333714003764	40.0	38.0	41.0	34.0	41.0
22	38.658421239022566	40.0	38.0	41.0	34.0	41.0
23	38.603498255149994	40.0	38.0	41.0	34.0	41.0
24	38.55770041900275	40.0	38.0	41.0	34.0	41.0
25	38.44466418103465	40.0	38.0	41.0	34.0	41.0
26	38.347281853957845	40.0	38.0	41.0	34.0	41.0
27	38.26519233141406	40.0	38.0	41.0	34.0	41.0
28	38.17963399930763	40.0	38.0	41.0	34.0	41.0
29	38.108023524636806	40.0	38.0	41.0	33.0	41.0
30	38.054453446128505	40.0	38.0	41.0	33.0	41.0
31	37.963070657472215	40.0	37.0	41.0	33.0	41.0
32	37.86864482652968	40.0	37.0	41.0	33.0	41.0
33	37.77590415859234	40.0	37.0	41.0	33.0	41.0
34	37.69555252257163	40.0	37.0	41.0	33.0	41.0
35	37.63758550162945	40.0	37.0	41.0	33.0	41.0
36	37.55622615006904	40.0	37.0	41.0	33.0	41.0
37	37.481355770340336	40.0	37.0	41.0	32.0	41.0
38	37.39392127682434	40.0	37.0	41.0	32.0	41.0
39	37.29146157549809	40.0	37.0	41.0	31.0	41.0
40	37.17827810959329	39.0	36.0	41.0	31.0	41.0
41	37.07587113178492	39.0	36.0	41.0	31.0	41.0
42	36.97606750201941	39.0	36.0	41.0	31.0	41.0
43	35.31253307654659	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	2.0
13	1.0
14	2.0
15	4.0
16	8.0
17	7.0
18	14.0
19	39.0
20	78.0
21	188.0
22	395.0
23	646.0
24	1156.0
25	1967.0
26	2987.0
27	4360.0
28	6663.0
29	9416.0
30	12710.0
31	16765.0
32	22527.0
33	29485.0
34	40031.0
35	55173.0
36	82393.0
37	138505.0
38	275602.0
39	304117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35599715094047	17.521616642327633	11.793355643008066	26.329030563723833
2	16.821090202975597	20.29984759918985	37.240610239901955	25.638451957932602
3	18.191205319305563	22.352980967804832	30.857383878938844	28.59842983395076
4	12.997341938872554	14.882754833652328	36.10218016720319	36.01772306027193
5	13.916621238226739	37.492887299004025	35.20806888675784	13.382422576011397
6	35.224781247139994	36.62404351580313	14.761490742546087	13.389684494510787
7	30.057975973992384	31.065790992037755	21.054092339770243	17.822140694199618
8	27.5475406965871	36.72909263820525	18.801604386596686	16.921762278610963
9	27.00170306910561	13.243650297838135	18.320626633931663	41.43401999912459
10	14.443756938613909	27.884374340956025	35.07506635204985	22.596802368380214
11	37.46702293174592	20.81086780920851	21.881553135358182	19.840556123687385
12	20.83315095638472	24.852772063300055	31.99601290830883	22.3180640720064
13	31.473254254688417	19.12630167402143	24.8225306492752	24.577913422014955
14	21.435790713498413	19.17285753508601	27.34102367186474	32.05032807955083
15	24.886793654078016	28.213349196811922	22.411673185813594	24.48818396329647
16	24.354882993581658	26.41478088901799	24.579007683706642	24.65132843369371
17	23.97049870479207	26.6059782500567	26.019255026640298	23.40426801851093
18	23.833119123317324	25.986526654225244	26.54221263693193	23.638141585525503
19	24.862123026847215	25.802790168357138	26.410702277258057	22.92438452753759
20	24.132151000155186	25.65834762505422	26.455567006617297	23.753934368173297
21	23.81988850468145	25.709777924563586	26.72435746943031	23.745976101324654
22	24.33508680479565	25.216962249961206	26.13564467930174	24.3123062659414
23	23.761793156686338	25.285005431517128	26.6293556589246	24.32384575287194
24	23.711457118868655	25.49003028120536	26.59841789655049	24.2000947033755
25	24.18925156479422	25.18572605258027	26.410801755593667	24.214220627031846
26	23.726279390874254	25.57717330319803	26.71152476413687	23.985022541790848
27	24.24088082097481	25.910624684156286	26.14071807441775	23.707776420451154
28	23.9302099788708	25.87650361504272	26.344748140749907	23.848538265336575
29	23.823668681434558	26.13773372434951	26.43656664451616	23.602030949699774
30	24.07156869376987	26.079240463011967	26.096947606750202	23.75224323646796
31	23.94930981930755	25.430343279840518	26.619805738706226	24.00054116214571
32	23.538862206588647	26.042135043830157	26.37648172980888	24.042521019772316
33	23.712949293902774	25.532607008845616	26.463425795130334	24.291017902121276
34	24.00153594550179	25.457401387125913	26.821448324983788	23.719614342388514
35	23.811731281161588	25.762004050757824	26.59941267990657	23.826851988174017
36	23.812228672839627	25.4461603352022	26.722467381053754	24.01914361090442
37	23.732248091010742	25.1516049834667	26.69302179371377	24.423125131808796
38	23.651073769154554	25.24143392052079	26.94689050618556	24.160601804139095
39	23.26857956874152	25.128625487941235	27.332866448344877	24.269928494972365
40	23.661220559386575	24.98199442125494	27.498696833803532	23.858088185554948
41	23.260422345221656	24.767121216341508	27.747193716152495	24.22526272228434
42	22.847885687454987	25.006963483492566	27.74858641285101	24.39656441620144
43	22.7667113655988	24.41874808504204	27.69397280660218	25.120567742756982
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	73.0
1	113.5
2	154.0
3	598.0
4	1042.0
5	1042.0
6	1325.0
7	1608.0
8	1431.0
9	1254.0
10	1686.5
11	2119.0
12	2119.0
13	3299.5
14	4480.0
15	7388.5
16	10297.0
17	8403.5
18	6510.0
19	6510.0
20	7293.0
21	8076.0
22	7271.5
23	6467.0
24	7963.0
25	9459.0
26	9459.0
27	11630.0
28	13801.0
29	15961.5
30	18122.0
31	20922.0
32	23722.0
33	23722.0
34	27182.0
35	30642.0
36	34983.0
37	39324.0
38	44233.0
39	49142.0
40	49142.0
41	53877.5
42	58613.0
43	63589.5
44	68566.0
45	73444.0
46	78322.0
47	78322.0
48	82423.5
49	86525.0
50	88468.5
51	90412.0
52	88568.5
53	86725.0
54	86725.0
55	82231.0
56	77737.0
57	71898.5
58	66060.0
59	60195.0
60	54330.0
61	54330.0
62	47099.5
63	39869.0
64	33500.0
65	27131.0
66	22430.0
67	17729.0
68	17729.0
69	14344.0
70	10959.0
71	8880.0
72	6801.0
73	5406.5
74	4012.0
75	4012.0
76	3173.5
77	2335.0
78	1822.5
79	1310.0
80	1018.5
81	727.0
82	727.0
83	575.5
84	424.0
85	325.0
86	226.0
87	158.0
88	90.0
89	90.0
90	59.0
91	28.0
92	21.0
93	14.0
94	10.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1005244.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.0126382876306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.387646541261	58.92826764984096
2	8.100752307678242	11.181085777349745
3	2.521571819368252	5.220609716590312
4	1.214740415461334	3.3532976362239655
5	0.704202088262253	2.4299421999318485
6	0.45340053790870344	1.8774220393266288
7	0.31612222141064195	1.5271499964626434
8	0.23882968992464423	1.3185813602493153
9	0.18711869995654407	1.1622199641257387
>10	0.8422724836919294	9.731948318768504
>50	0.027283834375530613	1.2689192402404195
>100	0.004905197429848275	0.5015082397282078
>500	5.770816355010034E-4	0.23208558477470195
>1k	4.328112266257525E-4	0.643089142882797
>5k	1.4427040887525085E-4	0.6238731335041966
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6266	0.6233312509201746	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2565	0.25516193083470284	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2350	0.2337740886789675	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1544	0.15359455017886206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	9.947833560807128E-5	0.0	0.0	0.0
11	0.0	9.947833560807128E-5	0.0	9.947833560807128E-5	0.0
12	0.0	9.947833560807128E-5	0.0	9.947833560807128E-5	9.947833560807128E-5
13	0.0	9.947833560807128E-5	0.0	1.9895667121614256E-4	1.9895667121614256E-4
14	0.0	9.947833560807128E-5	0.0	1.9895667121614256E-4	2.984350068242138E-4
15	0.0	9.947833560807128E-5	0.0	2.984350068242138E-4	2.984350068242138E-4
16	0.0	9.947833560807128E-5	0.0	5.968700136484277E-4	2.984350068242138E-4
17	0.0	9.947833560807128E-5	0.0	7.958266848645702E-4	2.984350068242138E-4
18	0.0	9.947833560807128E-5	0.0	8.953050204726415E-4	2.984350068242138E-4
19	0.0	9.947833560807128E-5	0.0	8.953050204726415E-4	2.984350068242138E-4
20	0.0	9.947833560807128E-5	0.0	0.0013926966985129978	2.984350068242138E-4
21	0.0	9.947833560807128E-5	0.0	0.0022880017189856393	3.979133424322851E-4
22	0.0	9.947833560807128E-5	0.0	0.003282785075066352	3.979133424322851E-4
23	0.0	9.947833560807128E-5	0.0	0.004078611759930922	3.979133424322851E-4
24	0.0	9.947833560807128E-5	0.0	0.0053718301228358484	3.979133424322851E-4
25	0.0	1.9895667121614256E-4	0.0	0.006466091814524633	3.979133424322851E-4
26	0.0	1.9895667121614256E-4	0.0	0.00785878851303763	4.973916780403564E-4
27	0.0	1.9895667121614256E-4	0.0	0.014623315334386478	4.973916780403564E-4
28	0.0	1.9895667121614256E-4	0.0	0.04665533940018543	4.973916780403564E-4
29	0.0	1.9895667121614256E-4	0.0	0.11002303918252683	4.973916780403564E-4
30	0.0	1.9895667121614256E-4	0.0	0.18224431083398657	4.973916780403564E-4
31	0.0	1.9895667121614256E-4	0.0	0.44635929187341583	4.973916780403564E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTGCG	45	0.0038254797	20.555555	10
TATACTA	105	2.2586391E-8	19.38095	5
CTATCGG	50	0.0070342836	18.5	1
TCTAGGT	90	2.1516698E-6	18.5	3
CTTATAC	575	0.0	18.017391	37
CACGATC	75	2.0669407E-4	17.266666	26
CTAATCT	65	0.0015797473	17.076923	4
CTAAGAC	175	7.2759576E-12	16.914286	3
CCCCTAT	90	4.4450644E-5	16.444445	1
TAAGACT	160	6.2937033E-10	16.1875	4
GTAGCGT	70	0.0025921897	15.857143	24
ATACCGG	70	0.0025921897	15.857143	6
CTTACAC	245	0.0	15.857142	3
TAGACTC	130	2.5878217E-7	15.653846	5
GTCTTAC	130	2.5878217E-7	15.653846	1
AACGCAG	2465	0.0	15.610547	5
ATAATAC	95	7.058267E-5	15.578948	3
GGTATAT	95	7.058267E-5	15.578948	1
GTGTAGA	205	5.456968E-12	15.341463	1
ACCGTCC	145	5.342372E-8	15.310346	8
>>END_MODULE